Ser114
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Home > Phosphorylation Site Page: > Ser114  -  eNOS (human)

Site Information
RKLQGRPsPGPPAPE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448638

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 ) , phospho-antibody ( 5 , 6 ) , western blotting ( 5 , 6 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
breast ( 1 ) , HAEC (endothelial) ( 6 ) , liver ( 2 ) , stromal ( 5 )

Upstream Regulation
Regulatory protein:
PTEN (human) ( 6 )
Putative in vivo kinases:
CDK5 (human) ( 4 )
Kinases, in vitro:
CDK5 (human) ( 4 )
Treatments:
seliciclib ( 4 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 4 ) , intracellular localization ( 5 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

3

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

4

Lee CH, et al. (2010) CDK5 phosphorylates eNOS at Ser-113 and regulates NO production. J Cell Biochem 110, 112-7
20213743   Curated Info

5

Klinz FJ, et al. (2005) Phospho-eNOS Ser-114 in human mesenchymal stem cells: constitutive phosphorylation, nuclear localization and upregulation during mitosis. Eur J Cell Biol 84, 809-18
16270749   Curated Info

6

Church JE, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol 52, 191-8
19962452   Curated Info