Thr461
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Home > Phosphorylation Site Page: > Thr461  -  CLOCK (human)

Site Information
KIPTDTstPPRQHLP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469131

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 4 , 5 ) , mutation of modification site ( 2 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:
melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:
'brain, forebrain' ( 2 ) , 293 (epithelial) ( 2 ) , HeLa (cervical) ( 4 ) , NCI-H1703 (squamous) ( 5 ) , WM239A (melanocyte) ( 1 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (human) ( 2 )
Kinases, in vitro:
CDK5 (human) ( 2 )

Downstream Regulation
Effects of modification on CLOCK:
intracellular localization ( 2 )
Effects of modification on biological processes:
transcription, induced ( 2 )

References 

1

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

2

Kwak Y, et al. (2013) Cyclin-dependent kinase 5 (Cdk5) regulates the function of CLOCK protein by direct phosphorylation. J Biol Chem 288, 36878-89
24235147   Curated Info

3

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

4

Moritz A (2008) CST Curation Set: 5061; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

5

Possemato A (2006) CST Curation Set: 1665; Year: 2006; Biosample/Treatment: cell line, NCI-H1703/serum starved; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

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