Ser404
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Home > Phosphorylation Site Page: > Ser404  -  GR (human)

Site Information
SMRPDVSsPPSSSST   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 5044100

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 4 ) , western blotting ( 1 , 4 )
Disease tissue studied:
bone cancer ( 4 ) , liver cancer ( 1 , 4 ) , lung cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
GSK3B (human) ( 1 , 4 )
Kinases, in vitro:
GSK3B (human) ( 4 )
Treatments:
BIO ( 1 ) , compound A ( 1 ) , dexamethasone ( 1 , 4 ) , GSK-3_inhibitor_X ( 4 ) , hydrocortisone ( 1 )

Downstream Regulation
Effects of modification on GR:
activity, inhibited ( 4 ) , intracellular localization ( 1 , 4 ) , molecular association, regulation ( 1 , 4 ) , protein conformation ( 4 ) , protein degradation ( 1 , 4 )
Effects of modification on biological processes:
apoptosis, inhibited ( 4 ) , transcription, induced ( 4 )
Induce interaction with:
FBXW7 (human) ( 1 ) , p300 (human) ( 4 )
Inhibit interaction with:
NFkB-p65 (human) ( 4 )

References 

1

Wilkinson L, Verhoog N, Louw A (2018) Novel role for receptor dimerization in post-translational processing and turnover of the GRĪ±. Sci Rep 8, 14266
30250038   Curated Info

2

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

3

Possemato A (2010) CST Curation Set: 9642; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSPX(I/L/M/V)
Curated Info

4

Galliher-Beckley AJ, Williams JG, Collins JB, Cidlowski JA (2008) Glycogen Synthase Kinase 3{beta}-Mediated Serine Phosphorylation of the Human Glucocorticoid Receptor Redirects Gene Expression Profiles. Mol Cell Biol 28, 7309-22
18838540   Curated Info