Thr9
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Home > Phosphorylation Site Page: > Thr9  -  TIFA (human)

Site Information
TSFEDADtEETVTCL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 50409900

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 6 ) , flow cytometry ( 2 ) , immunoprecipitation ( 2 , 3 , 5 , 6 ) , microscopy-colocalization with upstream kinase ( 5 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 1 , 3 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:
gastric cancer ( 3 ) , gastric carcinoma ( 3 )
Relevant cell line - cell type - tissue:
AGS (gastric) ( 3 ) , E.coli (bacterial) ( 7 ) , HEK293T (epithelial) ( 1 , 2 , 4 , 5 , 6 , 7 ) , HeLa (cervical) ( 2 , 5 )

Upstream Regulation
Putative in vivo kinases:
AlphaK3 (human) ( 1 ) , AurA (human) ( 5 )
Kinases, in vitro:
Akt1 (human) ( 6 ) , AlphaK3 (human) ( 1 ) , AurA (human) ( 5 )
Treatments:
ADP ( 1 ) , etoposide ( 2 ) , MK5108 ( 5 ) , VX-680 ( 5 )

Downstream Regulation
Effects of modification on TIFA:
activity, induced ( 4 ) , intracellular localization ( 3 ) , molecular association, regulation ( 3 , 6 , 7 ) , protein conformation ( 4 , 5 , 6 , 7 ) , ubiquitination ( 2 )
Induce interaction with:
CASP1 (human) ( 6 )

References 

1

Zhou P, et al. (2018) Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose. Nature 561, 122-126
30111836   Curated Info

2

Fu J, et al. (2018) TRAF-interacting protein with forkhead-associated domain (TIFA) transduces DNA damage-induced activation of NF-κB. J Biol Chem
29581234   Curated Info

3

Zimmermann S, et al. (2017) ALPK1- and TIFA-Dependent Innate Immune Response Triggered by the Helicobacter pylori Type IV Secretion System. Cell Rep 20, 2384-2395
28877472   Curated Info

4

Gaudet RG, et al. (2017) Innate Recognition of Intracellular Bacterial Growth Is Driven by the TIFA-Dependent Cytosolic Surveillance Pathway. Cell Rep 19, 1418-1430
28514661   Curated Info

5

Wei TW, et al. (2017) Aurora A and NF-κB Survival Pathway Drive Chemoresistance in Acute Myeloid Leukemia via the TRAF-Interacting Protein TIFA. Cancer Res 77, 494-508
28069801   Curated Info

6

Lin TY, et al. (2016) TIFA as a crucial mediator for NLRP3 inflammasome. Proc Natl Acad Sci U S A 113, 15078-15083
27965388   Curated Info

7

Weng JH, et al. (2015) Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses. Biochemistry 54, 6219-29
26389808   Curated Info