Thr74
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Home > Phosphorylation Site Page: > Thr74  -  Bcl-2 (human)

Site Information
ARtsPLQtPAAPGAA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448632

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 2 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 3 ) , western blotting ( 3 , 4 )
Relevant cell line - cell type - tissue:
COS (fibroblast) ( 4 ) , epithelial-'kidney, tubule' ( 2 ) , HeLa (cervical) ( 3 ) , HUVEC (endothelial) ( 3 ) , WEHI-7 (T lymphocyte) ( 1 )

Upstream Regulation
Regulatory protein:
JNK3 (mouse) ( 4 ) , RAC1 (human) ( 4 )
Putative in vivo kinases:
ERK2 (human) ( 3 )
Kinases, in vitro:
ERK1 (human) ( 3 ) , JNK3 (mouse) ( 4 )

Downstream Regulation
Effects of modification on Bcl-2:
protein stabilization ( 3 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
apoptosis, altered ( 2 ) , apoptosis, induced ( 3 ) , apoptosis, inhibited ( 1 )

References 

1

Huang ST, Cidlowski JA (2002) Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes. FASEB J 16, 825-32
12039864   Curated Info

2

Thomas A, Giesler T, White E (2000) p53 mediates bcl-2 phosphorylation and apoptosis via activation of the Cdc42/JNK1 pathway. Oncogene 19, 5259-69
11077443   Curated Info

3

Breitschopf K, et al. (2000) Posttranslational modification of Bcl-2 facilitates its proteasome-dependent degradation: molecular characterization of the involved signaling pathway. Mol Cell Biol 20, 1886-96
10669763   Curated Info

4

Maundrell K, et al. (1997) Bcl-2 undergoes phosphorylation by c-Jun N-terminal kinase/stress-activated protein kinases in the presence of the constitutively active GTP-binding protein Rac1. J Biol Chem 272, 25238-42
9312139   Curated Info