Thr562
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Home > Phosphorylation Site Page: > Thr562  -  p90RSK (mouse)

Site Information
AENGLLMtPCYtANF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447993

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 8 , 9 , 10 , 11 , 12 ) , western blotting ( 8 , 9 , 10 , 11 )
Disease tissue studied:
lymphoma ( 10 ) , B cell lymphoma ( 10 ) , pancreatic cancer ( 8 ) , pancreatic carcinoma ( 8 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 ) , 'brain, embryonic' ( 5 ) , A20 (B lymphocyte) ( 10 ) , B lymphocyte-spleen ( 10 ) , Bal 17 (B lymphocyte) ( 10 ) , beta-pancreas ( 8 ) , brain ( 6 ) , epithelial ( 12 ) , liver ( 7 ) , macrophage-peritoneum ( 4 ) , MIN6 (pancreatic) ( 8 ) , RAW 264.7 (macrophage) ( 2 ) , Swiss 3T3 (fibroblast) ( 9 , 11 )

Upstream Regulation
Regulatory protein:
ARRB1 (mouse) ( 8 ) , PRKD1 (mouse) ( 11 )
Treatments:
anti-IgM ( 10 ) , bombesin ( 9 , 11 ) , EGF ( 11 ) , GF109203X ( 9 ) , GLP-1 ( 8 ) , IL-33 ( 2 ) , phorbol_ester ( 10 ) , rottlerin ( 10 ) , U0126 ( 8 ) , vasopressin ( 11 )

References 

1

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

2

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

5

Guo A (2011) CST Curation Set: 12476; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

6

Guo A (2011) CST Curation Set: 12477; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

7

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

8

Quoyer J, et al. (2010) GLP-1 mediates antiapoptotic effect by phosphorylating Bad through a beta-arrestin 1-mediated ERK1/2 activation in pancreatic beta-cells. J Biol Chem 285, 1989-2002
19915011   Curated Info

9

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

10

Blois JT, et al. (2004) B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta. J Biol Chem 279, 30123-32
15138267   Curated Info

11

Sinnett-Smith J, et al. (2004) Protein kinase D potentiates DNA synthesis induced by Gq-coupled receptors by increasing the duration of ERK signaling in swiss 3T3 cells. J Biol Chem 279, 16883-93
14963034   Curated Info

12

Miyagawa S, Katsu Y, Watanabe H, Iguchi T (2004) Estrogen-independent activation of erbBs signaling and estrogen receptor alpha in the mouse vagina exposed neonatally to diethylstilbestrol. Oncogene 23, 340-9
14647453   Curated Info