Ser363
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Home > Phosphorylation Site Page: > Ser363  -  p90RSK (human)

Site Information
TSRtPKDsPGIPPsA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447989

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 , 35 , 40 ) , mass spectrometry ( 2 , 3 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 15 , 16 , 17 , 19 , 20 , 21 , 23 , 24 , 25 , 26 , 27 , 29 , 30 , 31 , 32 , 33 , 34 , 36 , 37 , 39 , 41 , 47 , 50 ) , mutation of modification site ( 1 , 40 ) , phospho-antibody ( 1 , 5 , 8 , 22 , 28 , 35 , 38 , 40 , 42 , 43 , 44 , 45 , 46 , 47 , 49 ) , western blotting ( 1 , 5 , 8 , 22 , 28 , 35 , 38 , 40 , 42 , 43 , 44 , 47 )
Disease tissue studied:
breast cancer ( 5 , 7 , 16 ) , breast adenocarcinoma ( 5 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 7 ) , cervical cancer ( 31 ) , cervical adenocarcinoma ( 31 ) , colorectal cancer ( 41 ) , colorectal carcinoma ( 41 ) , gastric cancer ( 19 , 29 , 38 ) , gastric carcinoma ( 19 , 29 , 38 ) , lung cancer ( 11 , 16 , 35 , 41 ) , non-small cell lung cancer ( 16 , 35 ) , non-small cell lung adenocarcinoma ( 11 ) , lymphoma ( 1 , 44 ) , B cell lymphoma ( 1 ) , non-Hodgkin's lymphoma ( 1 ) , mantle cell lymphoma ( 1 ) , T cell lymphoma ( 44 ) , ovarian cancer ( 7 ) , prostate cancer ( 8 , 43 )
Relevant cell line - cell type - tissue:
293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 25 ) , 293 (epithelial) [AT1 (human), transfection] ( 23 ) , 293 (epithelial) ( 8 , 35 , 42 ) , 293E (epithelial) ( 47 ) , A431 (epithelial) ( 50 ) , A498 (renal) ( 27 ) , A549 (pulmonary) ( 12 , 41 ) , AP-1 (fibroblast) ( 40 ) , BaF3 ('B lymphocyte, precursor') [RSK3 (human)] ( 39 ) , breast ( 3 , 7 ) , BT-20 (breast cell) ( 16 ) , BT-549 (breast cell) ( 16 ) , Calu 6 (pulmonary) ( 16 ) , COS (fibroblast) ( 28 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 17 ) , Flp-In T-Rex-293 (epithelial) ( 17 ) , GRANTA-519 (B lymphocyte) ( 1 ) , H2009 (pulmonary) ( 16 ) , H2077 (pulmonary) ( 16 ) , H2887 (pulmonary) ( 16 ) , H322M (pulmonary) ( 16 ) , HCC1359 (pulmonary) ( 16 ) , HCC1937 (breast cell) ( 16 ) , HCC366 (pulmonary) ( 16 ) , HCC4006 (pulmonary) ( 16 ) , HCC78 (pulmonary) ( 16 ) , HCT116 (intestinal) ( 33 , 41 ) , HeLa (cervical) ( 2 , 6 , 15 , 21 , 28 , 32 , 37 ) , HeLa S3 (cervical) ( 31 , 36 ) , HeLa_Meta (cervical) ( 26 ) , HeLa_Pro (cervical) ( 26 ) , HeLa_Telo (cervical) ( 26 ) , HOP62 (pulmonary) ( 16 ) , HUES-9 ('stem, embryonic') ( 20 ) , HUT-78 (T lymphocyte) ( 44 ) , JAR (placental) ( 22 ) , JB (epithelial) ( 46 ) , JEKO-1 (B lymphocyte) ( 1 ) , Jurkat (T lymphocyte) ( 13 , 24 , 30 , 41 , 44 ) , K562 (erythroid) ( 15 ) , liver ( 10 ) , LNCaP (prostate cell) ( 43 ) , lung ( 11 ) , M14-MEL (melanocyte) ( 49 ) , MALME-3M (melanocyte) ( 49 ) , MCF-7 (breast cell) ( 16 ) , MCF10A1 (epithelial) ( 5 ) , MDA-MB-231 (breast cell) ( 16 ) , MDA-MB-361 (breast cell) ( 5 ) , MDA-MB-435S (breast cell) ( 33 ) , MDA-MB-468 (breast cell) ( 16 ) , MKN-45 (gastric) ( 19 , 29 ) , MOLT-4 (T lymphocyte) ( 44 ) , MV4-11 (macrophage) ( 33 ) , myocyte-heart ( 45 ) , NCI-H1299 (pulmonary) ( 35 ) , NCI-H1395 (pulmonary) ( 16 ) , NCI-H1568 (pulmonary) ( 16 ) , NCI-H157 (pulmonary) ( 16 ) , NCI-H1648 (pulmonary) ( 16 ) , NCI-H1666 (pulmonary) ( 16 ) , NCI-H2030 (pulmonary) ( 16 ) , NCI-H322 (pulmonary) ( 16 ) , NCI-H520 (squamous) ( 16 ) , NCI-H647 (pulmonary) ( 16 ) , NHEM (melanocyte) ( 49 ) , ovary ( 7 ) , PC3 (prostate cell) ( 8 ) , PC9 (pulmonary) ( 16 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 9 ) , SH-SY5Y (neural crest) ( 9 ) , SK-MEL5 (melanocyte) ( 49 ) , SKBr3 (breast cell) ( 5 ) , SNU-484 (gastric) ( 38 ) , SNU-620 (gastric) ( 38 ) , UACC-62 (melanocyte) ( 49 ) , WM115 (melanocyte) ( 34 )

Upstream Regulation
Regulatory protein:
ATP6V1B2 (mouse) ( 42 ) , DUSP16 (human) ( 28 )
Treatments:
acidosis ( 40 ) , AG1478 ( 48 ) , angiotensin_2 ( 25 , 48 ) , anti-IgM ( 1 ) , dronabinol ( 44 ) , EGF ( 2 , 22 , 32 , 43 , 46 , 47 , 48 ) , enzastaurin ( 38 ) , FGF2 ( 5 ) , galardin ( 48 ) , H2O2 ( 45 ) , HB-EGF ( 22 ) , idelalisib ( 1 ) , ischemia ( 7 ) , LRRK2-IN-1 ( 9 ) , LY294002 ( 43 ) , LY333531 ( 45 ) , PD184352 ( 49 ) , PD98059 ( 43 , 49 ) , phorbol_ester ( 28 ) , PKCbeta_C2-4_inhibitor ( 45 ) , PTX ( 44 ) , rimonabant ( 44 ) , SII_angiotensin_2 ( 23 ) , SR_144528 ( 44 ) , U0126 ( 34 ) , UV ( 46 ) , Z-VAD-FMK ( 44 )

Downstream Regulation
Effects of modification on p90RSK:
enzymatic activity, induced ( 8 )

References 

1

Yang Q, et al. (2017) Idelalisib Impacts Cell Growth through Inhibiting Translation-Regulatory Mechanisms in Mantle Cell Lymphoma. Clin Cancer Res 23, 181-192
27342398   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
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3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
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4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
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5

Czaplinska D, et al. (2014) Phosphorylation of RSK2 at Tyr529 by FGFR2-p38 enhances human mammary epithelial cells migration. Biochim Biophys Acta 1843, 2461-70
25014166   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Wu CF, et al. (2014) RSK promotes G2/M transition through activating phosphorylation of Cdc25A and Cdc25B. Oncogene 33, 2385-94
23708659   Curated Info

9

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

10

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
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11

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
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12

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
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13

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
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14

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
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15

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
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16

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
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17

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
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18

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
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19

Guo A (2011) CST Curation Set: 11298; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

20

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

21

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
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22

Wright JK, et al. (2010) HER1 Signaling Mediates Extravillous Trophoblast Differentiation in Humans. Biol Reprod 83, 1036-45
20739666   Curated Info

23

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

24

Possemato A (2010) CST Curation Set: 10140; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

25

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

26

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
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27

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
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28

Masuda K, et al. (2010) MKP-7, a JNK phosphatase, blocks ERK-dependent gene activation by anchoring phosphorylated ERK in the cytoplasm. Biochem Biophys Res Commun 393, 201-6
20122898   Curated Info

29

Moritz A (2010) CST Curation Set: 9235; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

30

Moritz A (2010) CST Curation Set: 8865; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

31

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

32

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
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33

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

34

Old WM, et al. (2009) Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Mol Cell 34, 115-31
19362540   Curated Info

35

Casar B, Pinto A, Crespo P (2008) Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes. Mol Cell 31, 708-21
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36

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
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37

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
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38

Lee KW, et al. (2008) Enzastaurin, a protein kinase C beta inhibitor, suppresses signaling through the ribosomal S6 kinase and bad pathways and induces apoptosis in human gastric cancer cells. Cancer Res 68, 1916-26
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39

Kang S, et al. (2007) FGFR3 activates RSK2 to mediate hematopoietic transformation through tyrosine phosphorylation of RSK2 and activation of the MEK/ERK pathway. Cancer Cell 12, 201-14
17785202   Curated Info

40

Malo ME, Li L, Fliegel L (2007) Mitogen-activated protein kinase-dependent activation of the Na+/H+ exchanger is mediated through phosphorylation of amino acids Ser770 and Ser771. J Biol Chem 282, 6292-9
17209041   Curated Info

41

Wissing J, et al. (2007) Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. Mol Cell Proteomics 6, 537-47
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42

Li G, et al. (2006) A novel cellular survival factor--the B2 subunit of vacuolar H+-ATPase inhibits apoptosis. Cell Death Differ 13, 2109-17
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43

Sastry KS, Karpova Y, Kulik G (2006) Epidermal growth factor protects prostate cancer cells from apoptosis by inducing BAD phosphorylation via redundant signaling pathways. J Biol Chem 281, 27367-77
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44

Jia W, et al. (2006) Delta9-tetrahydrocannabinol-induced apoptosis in Jurkat leukemia T cells is regulated by translocation of Bad to mitochondria. Mol Cancer Res 4, 549-62
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45

Itoh S, et al. (2005) Role of p90 ribosomal S6 kinase (p90RSK) in reactive oxygen species and protein kinase C beta (PKC-beta)-mediated cardiac troponin I phosphorylation. J Biol Chem 280, 24135-42
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46

Choi HS, et al. (2005) Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha. J Biol Chem 280, 13545-53
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47

Ballif BA, et al. (2005) Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proc Natl Acad Sci U S A 102, 667-72
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48

Shah BH, et al. (2004) Differential pathways of angiotensin II-induced extracellularly regulated kinase 1/2 phosphorylation in specific cell types: role of heparin-binding epidermal growth factor. Mol Endocrinol 18, 2035-48
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49

Eisenmann KM, et al. (2003) Mitogen-activated protein kinase pathway-dependent tumor-specific survival signaling in melanoma cells through inactivation of the proapoptotic protein bad. Cancer Res 63, 8330-7
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50

MS This site is one of 509 sites observed by D. Stover et al using MS/FTMS of peptides from lysates of A431 cells grown either in vitro or as xenografts in BALB/c nu/nu mice. These sites were previously unpublished until now (July 27 2006). 66 sites were previously published in: Stover DR, et al. Differential phosphoprofiles of EGF and EGFR kinase inhibitor-treated human tumor cells and mouse xenografts Clin Proteomics 2004 Mar 01; 1(1): 69-80.
Curated Info