Ser23
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Home > Phosphorylation Site Page: > Ser23  -  p53 (mouse)

Site Information
PLsQEtFsGLWKLLP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447529

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 9 ) , modification-specific antibody ( 11 ) , mutation of modification site ( 4 , 9 , 10 , 11 , 13 ) , phospho-antibody ( 3 , 5 , 6 , 7 , 11 , 12 , 13 ) , western blotting ( 3 , 5 , 6 , 7 , 9 , 11 , 12 )
Disease tissue studied:
leukemia ( 7 ) , acute myelogenous leukemia ( 7 ) , cancer, squamous cell carcinoma ( 11 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 10 ) , 'brain, embryonic' ( 5 ) , 'stem, embryonic' ( 12 ) , bone marrow [p53 (mouse), genetic knockin] ( 4 ) , brain [p53 (mouse), genetic knockin] ( 4 ) , epidermal ( 11 ) , ES (stem) ( 13 ) , MEF (fibroblast) [IGF1R (mouse)] ( 13 ) , MEF (fibroblast) [p53 (mouse), genetic knockin] ( 3 , 4 ) , MEF (fibroblast) [p53 (mouse)] ( 9 ) , MEF (fibroblast) ( 6 , 12 ) , T lymphocyte-thymus ( 10 ) , T lymphocyte-thymus [p53 (mouse)] ( 9 ) , testis [p53 (mouse), genetic knockin] ( 4 ) , thymocyte ( 12 ) , thymocyte [p53 (mouse), genetic knockin] ( 3 )

Upstream Regulation
Regulatory protein:
ATR (human) ( 7 ) , BRCA1 (mouse) ( 6 ) , HBpX (retrovirus) ( 7 ) , P38A (human) ( 7 )
Treatments:
adriamycin ( 8 ) , etoposide ( 5 ) , ionizing_radiation ( 3 , 6 , 10 , 11 , 12 , 13 ) , LLnL ( 12 ) , UV ( 11 , 13 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 4 ) , molecular association, regulation ( 4 ) , protein stabilization ( 10 , 12 )
Effects of modification on biological processes:
apoptosis, altered ( 4 , 9 , 10 ) , cell cycle regulation ( 4 ) , cell growth, altered ( 9 ) , transcription, inhibited ( 12 )
Inhibit interaction with:
MDM2 (mouse) ( 4 )

References 

1

Vaughan CA, et al. (2021) The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3. Nat Commun 12, 704
33514736   Curated Info

2

Song L, et al. (2011) p85α mediates p53 K370 acetylation by p300 and regulates its promoter-specific transactivity in the cellular UVB response. Oncogene 30, 1360-71
21057544   Curated Info

3

Lee MK, Tong WM, Wang ZQ, Sabapathy K (2011) Serine 312 phosphorylation is dispensable for wild-type p53 functions in vivo. Cell Death Differ 18, 214-21
20671749   Curated Info

4

Liu D, et al. (2010) Puma is required for p53-induced depletion of adult stem cells. Nat Cell Biol 12, 993-8
20818388   Curated Info

5

Nam C, Doi K, Nakayama H (2010) Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway. Histol Histopathol 25, 485-93
20183801   Curated Info

6

Kim SS, et al. (2009) Impaired skin and mammary gland development and increased gamma-irradiation-induced tumorigenesis in mice carrying a mutation of S1152-ATM phosphorylation site in Brca1. Cancer Res 69, 9291-300
19996295   Curated Info

7

Wang WH, Hullinger RL, Andrisani OM (2008) Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283, 25455-67
18606816   Curated Info

8

Qin J, et al. (2008) Protein phosphatase-2A is a target of epigallocatechin-3-gallate and modulates p53-Bak apoptotic pathway. Cancer Res 68, 4150-62
18519674   Curated Info

9

Chao C, Herr D, Chun J, Xu Y (2006) Ser18 and 23 phosphorylation is required for p53-dependent apoptosis and tumor suppression. EMBO J 25, 2615-22
16757976   Curated Info

10

MacPherson D, et al. (2004) Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. EMBO J 23, 3689-99
15343266   Curated Info

11

Knights CD, Liu Y, Appella E, Kulesz-Martin M (2003) Defective p53 post-translational modification required for wild type p53 inactivation in malignant epithelial cells with mdm2 gene amplification. J Biol Chem 278, 52890-900
14555661   Curated Info

12

Takai H, et al. (2002) Chk2-deficient mice exhibit radioresistance and defective p53-mediated transcription. EMBO J 21, 5195-205
12356735   Curated Info

13

Wu Z, et al. (2002) Mutation of mouse p53 Ser23 and the response to DNA damage. Mol Cell Biol 22, 2441-9
11909939   Curated Info

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