Thr187
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Home > Phosphorylation Site Page: > Thr187  -  p27Kip1 (mouse)

Site Information
NAGtVEQtPKKPGLR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447875

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 4 ) , microscopy-colocalization with upstream kinase ( 2 ) , mutation of modification site ( 2 , 3 , 4 , 6 ) , phospho-antibody ( 1 , 2 , 5 ) , western blotting ( 1 , 2 , 5 )
Disease tissue studied:
eye cancer ( 3 ) , retinoblastoma ( 3 )
Relevant cell line - cell type - tissue:
'stem, neural' ( 2 ) , 'stem, neural' [CDK5 (mouse), homozygous knockout] ( 2 ) , BaF3 ('B lymphocyte, precursor') ( 1 ) , MEF (fibroblast) [IGF1R (mouse)] ( 6 ) , mesangial ( 4 ) , pituitary gland [genetic knockin] ( 3 ) , RB177 (retinal) ( 3 ) , VSMC-aorta ( 5 ) , Y79 (retinal) ( 3 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (mouse) ( 2 )
Kinases, in vitro:
CDK5 (mouse) ( 2 )
Treatments:
IL-3 ( 1 ) , ponasterone_A ( 1 )

Downstream Regulation
Effects of modification on p27Kip1:
ubiquitination ( 3 )
Effects of modification on biological processes:
apoptosis, altered ( 3 ) , cell cycle regulation ( 6 ) , cell differentiation, induced ( 2 ) , cell growth, altered ( 6 ) , cell growth, induced ( 2 )

References 

1

Bustany S, Tchakarska G, Sola B (2011) Cyclin D1 regulates p27(Kip1) stability in B cells. Cell Signal 23, 171-9
20837141   Curated Info

2

Zheng YL, et al. (2010) Phosphorylation of p27Kip1 at Thr187 by cyclin-dependent kinase 5 modulates neural stem cell differentiation. Mol Biol Cell 21, 3601-14
20810788   Curated Info

3

Wang H, et al. (2010) Skp2 is required for survival of aberrantly proliferating Rb1-deficient cells and for tumorigenesis in Rb1+/- mice. Nat Genet 42, 83-8
19966802   Curated Info

4

Wolf G, et al. (2003) Erk 1,2 phosphorylates p27(Kip1): Functional evidence for a role in high glucose-induced hypertrophy of mesangial cells. Diabetologia 46, 1090-9
12856081   Curated Info

5

Castro C, Díez-Juan A, Cortés MJ, Andrés V (2003) Distinct regulation of mitogen-activated protein kinases and p27Kip1 in smooth muscle cells from different vascular beds. A potential role in establishing regional phenotypic variance. J Biol Chem 278, 4482-90
12477734   Curated Info

6

Malek NP, et al. (2001) A mouse knock-in model exposes sequential proteolytic pathways that regulate p27Kip1 in G1 and S phase. Nature 413, 323-7
11565035   Curated Info