Ser325

Site Information
sFRVRASsDGEGTMs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447559

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 , 2 , 3 ) , 'fat, brown' ( 5 ) , heart ( 5 ) , kidney ( 5 ) , liver ( 4 , 5 ) , liver [leptin (mouse), homozygous knockout] ( 4 ) , lung ( 5 ) , spleen ( 5 )

Upstream Regulation
Treatments:	insulin ( 3 )

References
1	Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36 27681418 Curated Info
2	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
3	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
4	Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83 23140645 Curated Info
5	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info