Thr215

Site Information
tVNsLKAtPQsPKNR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 459786

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 )
Disease tissue studied:	anthrax infection ( 6 )
Relevant cell line - cell type - tissue:	32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 8 ) , 32Dcl3 (myeloid) ( 8 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , kidney ( 7 ) , liver ( 9 ) , lung ( 7 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 3 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 3 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) ( 3 , 5 ) , spleen ( 6 , 7 ) , testis ( 7 )

Upstream Regulation
Regulatory protein:	FLT3 (mouse) ( 8 ) , RICTOR (mouse) ( 3 )

References
1	Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32 28852199 Curated Info
2	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
3	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
4	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
5	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
6	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
7	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
8	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
9	Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93 17242355 Curated Info