Ser657
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Home > Phosphorylation Site Page: > Ser657  -  PKCA (mouse)

Site Information
QsDFEGFsyVNPQFV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448597

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 7 , 8 ) , phospho-antibody ( 3 , 5 , 6 , 9 , 10 , 11 ) , western blotting ( 3 , 5 , 6 , 9 , 10 )
Disease tissue studied:
leukemia ( 3 ) , chronic myelogenous leukemia ( 3 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 7 ) , aorta ( 9 ) , brain ( 8 ) , endothelial ( 6 ) , HUVEC (endothelial) ( 6 ) , K562 (erythroid) ( 3 ) , lung ( 6 ) , M1 (myeloid) ( 3 ) , mast-bone marrow ( 11 ) , MCP-5 (mast) ( 11 ) , MEF (fibroblast) ( 5 , 10 )

Upstream Regulation
Regulatory protein:
FKBP12 (mouse) ( 9 ) , GILZ (human) ( 3 ) , PTPRA (mouse) ( 10 ) , syndecan-4 (mouse) ( 6 )
Putative in vivo kinases:
PKCA (mouse) ( 11 )
Treatments:
H2O2 ( 10 ) , rapamycin ( 9 ) , ryanodine ( 9 )

Downstream Regulation
Effects of modification on biological processes:
signaling pathway regulation ( 6 )

References 

1

Bulek K, et al. (2019) IL-17A Recruits Rab35 to IL-17R to Mediate PKCĪ±-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility. J Immunol
30683702   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Joha S, et al. (2012) GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene 31, 1419-30
21804606   Curated Info

4

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

5

Xie X, et al. (2011) IkappaB kinase epsilon and TANK-binding kinase 1 activate AKT by direct phosphorylation. Proc Natl Acad Sci U S A 108, 6474-9
21464307   Curated Info

6

Partovian C, et al. (2008) Syndecan-4 regulates subcellular localization of mTOR Complex2 and Akt activation in a PKCalpha-dependent manner in endothelial cells. Mol Cell 32, 140-9
18851840   Curated Info

7

Guo A (2007) CST Curation Set: 3120; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

8

Guo A (2007) CST Curation Set: 3121; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

9

Long C, et al. (2007) FK506 binding protein 12/12.6 depletion increases endothelial nitric oxide synthase threonine 495 phosphorylation and blood pressure. Hypertension 49, 569-76
17261647   Curated Info

10

Hao Q, Rutherford SA, Low B, Tang H (2006) Selective regulation of hydrogen peroxide signaling by receptor tyrosine phosphatase-alpha. Free Radic Biol Med 41, 302-10
16814111   Curated Info

11

Kawakami Y, et al. (2003) A Ras activation pathway dependent on Syk phosphorylation of protein kinase C. Proc Natl Acad Sci U S A 100, 9470-5
12881490   Curated Info