Ser657
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Home > Phosphorylation Site Page: > Ser657  -  PKCA (human)

Site Information
QsDFEGFsyVNPQFV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448597

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 8 ) , [32P] bio-synthetic labeling ( 12 ) , electrophoretic mobility shift ( 12 ) , immunoassay ( 8 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 5 , 7 , 11 ) , mutation of modification site ( 1 , 13 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 8 , 10 , 12 , 13 , 14 ) , phosphopeptide mapping ( 12 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 8 , 10 , 12 , 13 , 14 )
Disease tissue studied:
breast cancer ( 1 ) , cervical cancer ( 11 ) , cervical adenocarcinoma ( 11 )
Relevant cell line - cell type - tissue:
'kidney, tubule' ( 3 ) , 293 (epithelial) ( 13 ) , cardiac ( 14 ) , cerebral artery ( 12 ) , HaCaT (keratinocyte) ( 6 ) , HEK293T (epithelial) ( 1 ) , HeLa S3 (cervical) ( 11 ) , liver ( 5 ) , lung ( 2 ) , MCF-7 (breast cell) ( 13 ) , MDA-MB-231 (breast cell) ( 1 ) , mesenchymal ( 2 ) , monocyte-blood ( 10 ) , retinal ganglion ( 8 ) , SKBR7 (breast cell) ( 1 )

Upstream Regulation
Regulatory protein:
MINK (human) ( 1 ) , RICTOR (human) ( 2 )
Treatments:
fumonisin_B1 ( 13 ) , Go_6976 ( 8 , 12 ) , hypoxia ( 3 ) , okadaic_acid ( 12 ) , phorbol_ester ( 6 , 13 ) , Shh ( 8 ) , tert-butylhydroperoxide ( 3 )

Downstream Regulation
Effects of modification on PKCA:
intracellular localization ( 13 )
Effects of modification on biological processes:
signaling pathway regulation ( 8 )

References 

1

Daulat AM, et al. (2016) PRICKLE1 Contributes to Cancer Cell Dissemination through Its Interaction with mTORC2. Dev Cell 37, 311-25
27184734   Curated Info

2

Walker NM, et al. (2016) Mechanistic Target of Rapamycin Complex 1 (mTORC1) and mTORC2 as Key Signaling Intermediates in Mesenchymal Cell Activation. J Biol Chem 291, 6262-71
26755732   Curated Info

3

Nowak G, Bakajsova D (2015) Protein Kinase C-α Interaction with F0F1-ATPase Promotes F0F1-ATPase Activity and Reduces Energy Deficits in Injured Renal Cells. J Biol Chem 290, 7054-66
25627689   Curated Info

4

Antal CE, et al. (2015) Cancer-associated protein kinase C mutations reveal kinase's role as tumor suppressor. Cell 160, 489-502
25619690   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Vivo M, et al. (2013) Mimicking p14ARF phosphorylation influences its ability to restrain cell proliferation. PLoS One 8, e53631
23308265   Curated Info

7

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

8

Guo D, et al. (2012) Protein kinase Cα and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog. Mol Cell Neurosci 50, 82-92
22521536   Curated Info

9

Cameron AJ, et al. (2011) mTORC2 targets AGC kinases through Sin1-dependent recruitment. Biochem J 439, 287-97
21806543   Curated Info

10

Wang H, et al. (2011) Convergence of the mammalian target of rapamycin complex 1- and glycogen synthase kinase 3-β-signaling pathways regulates the innate inflammatory response. J Immunol 186, 5217-26
21422248   Curated Info

11

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

12

Obara K, Ito Y, Shimada H, Nakayama K (2008) The relaxant effect of okadaic acid on canine basilar artery involves activation of PKCalpha and phosphorylation of the myosin light chain at Thr-9. Eur J Pharmacol 598, 87-93
18835557   Curated Info

13

Kitatani K, Idkowiak-Baldys J, Hannun YA (2007) Mechanism of inhibition of sequestration of protein kinase C alpha/betaII by ceramide. Roles of ceramide-activated protein phosphatases and phosphorylation/dephosphorylation of protein kinase C alpha/betaII on threonine 638/641. J Biol Chem 282, 20647-56
17504762   Curated Info

14

Stawowy P, et al. (2005) Protein kinase C epsilon mediates angiotensin II-induced activation of beta1-integrins in cardiac fibroblasts. Cardiovasc Res 67, 50-9
15949469   Curated Info