Thr642
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Home > Phosphorylation Site Page: > Thr642  -  PKCB (human)

Site Information
TRQPVELtPtDKLFI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447897

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 , 5 , 6 , 8 , 11 ) , mutation of modification site ( 1 , 12 ) , phospho-antibody ( 1 , 2 , 3 , 9 , 10 , 12 , 13 ) , western blotting ( 1 , 2 , 3 , 9 , 10 , 12 )
Disease tissue studied:
brain cancer ( 10 ) , glioblastoma ( 10 ) , glioblastoma multiforme ( 10 ) , glioma ( 10 ) , breast cancer ( 13 ) , HER2 positive breast cancer ( 4 ) , luminal A breast cancer ( 4 ) , luminal B breast cancer ( 4 ) , breast cancer, surrounding tissue ( 4 ) , breast cancer, triple negative ( 4 ) , colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lymphoma ( 5 ) , Burkitt's lymphoma ( 5 ) , fibrosarcoma of soft tissue ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 12 ) , BJAB (B lymphocyte) ( 5 ) , breast ( 4 ) , Caco-2 (intestinal) ( 3 ) , Calu-1 (squamous) ( 2 ) , HT1080 (fibroblast) ( 2 ) , K562 (erythroid) ( 8 ) , liver ( 6 ) , LN229 (glial) ( 10 ) , MCF-7 (breast cell) ( 12 , 13 ) , platelet-blood ( 11 ) , retinal ganglion ( 9 )

Upstream Regulation
Regulatory protein:
MDM2 (human) ( 1 )
Putative in vivo kinases:
mTOR (human) ( 1 )
Putative upstream phosphatases:
PPP1CA (human) ( 12 )
Treatments:
acadesine ( 3 ) , C6-ceramide ( 12 ) , compound_C ( 3 ) , estradiol ( 13 ) , fumonisin_B1 ( 12 ) , Go_6976 ( 9 ) , LY333531 ( 3 ) , NaB ( 3 ) , okadaic_acid ( 12 ) , phorbol_ester ( 1 , 12 ) , RSL3 ( 2 ) , Shh ( 9 ) , sphingomyelinase ( 12 ) , tautomycin ( 12 ) , Torin1 ( 1 )

Downstream Regulation
Effects of modification on PKCB:
enzymatic activity, induced ( 1 ) , intracellular localization ( 12 ) , molecular association, regulation ( 1 )
Induce interaction with:
CLTA (human) ( 1 )

References 

1

Min X, et al. (2023) PKCβII activation requires nuclear trafficking for phosphorylation and Mdm2-mediated ubiquitination. Life Sci Alliance 6
36717249   Curated Info

2

Chen X, et al. (2022) Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis. Autophagy, 1-21
35403545   Curated Info

3

Miao W, et al. (2016) Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. Int J Mol Sci 17
27735862   Curated Info

4

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

5

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
24667141   Curated Info

6

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

7

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

8

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

9

Guo D, et al. (2012) Protein kinase Cα and integrin-linked kinase mediate the negative axon guidance effects of Sonic hedgehog. Mol Cell Neurosci 50, 82-92
22521536   Curated Info

10

Fan QW, et al. (2009) EGFR signals to mTOR through PKC and independently of Akt in glioma. Sci Signal 2, ra4
19176518   Curated Info

11

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

12

Kitatani K, Idkowiak-Baldys J, Hannun YA (2007) Mechanism of inhibition of sequestration of protein kinase C alpha/betaII by ceramide. Roles of ceramide-activated protein phosphatases and phosphorylation/dephosphorylation of protein kinase C alpha/betaII on threonine 638/641. J Biol Chem 282, 20647-56
17504762   Curated Info

13

Castoria G, et al. (2004) Role of atypical protein kinase C in estradiol-triggered G1/S progression of MCF-7 cells. Mol Cell Biol 24, 7643-53
15314172   Curated Info

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