Ser279
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Home > Phosphorylation Site Page: > Ser279  -  HDAC5 (human)

Site Information
KVAERRssPLLRRKD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4788487

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 4 , 5 , 8 , 10 , 11 , 13 , 14 ) , mutation of modification site ( 10 , 12 ) , phospho-antibody ( 1 , 12 ) , western blotting ( 1 , 10 , 12 )
Disease tissue studied:
bone cancer ( 10 ) , cervical cancer ( 14 ) , cervical adenocarcinoma ( 14 ) , lung cancer ( 13 ) , non-small cell lung cancer ( 13 ) , pancreatic ductal adenocarcinoma ( 4 )
Relevant cell line - cell type - tissue:
'pancreatic, ductal'-pancreas ( 4 ) , 293 (epithelial) ( 10 ) , ARVM (myocyte) ( 1 ) , COS (fibroblast) ( 12 ) , HeLa (cervical) ( 3 , 8 , 11 ) , HeLa S3 (cervical) ( 14 ) , Jurkat (T lymphocyte) ( 5 ) , K562 (erythroid) ( 8 ) , lung ( 13 ) , U2OS (bone cell) ( 10 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (human) ( 9 ) , PKACA (human) ( 7 , 12 )
Kinases, in vitro:
PKACA (human) ( 12 )
Treatments:
CGP_20712A ( 1 ) , colforsin ( 9 , 12 ) , isoproterenol ( 1 ) , N6-benzoyl-cAMP ( 1 ) , nocodazole ( 14 ) , okadaic_acid ( 1 ) , seliciclib ( 9 )

Downstream Regulation
Effects of modification on HDAC5:
enzymatic activity, induced ( 10 ) , intracellular localization ( 9 , 10 , 12 ) , molecular association, regulation ( 10 , 12 ) , protein conformation ( 12 )
Effects of modification on biological processes:
neural plasticity ( 9 ) , transcription, inhibited ( 12 )
Induce interaction with:
GPS2 (human) ( 10 ) , HDAC3 (human) ( 10 ) , SMRT (human) ( 10 ) , TBL1X (human) ( 10 ) , TBL1XR1 (human) ( 10 )
Inhibit interaction with:
14-3-3 beta (human) ( 12 ) , N-CoR1 (human) ( 10 )

Disease / Diagnostics Relevance
Relevant diseases:
cardiomyopathy ( 12 )

References 

1

Weeks KL, et al. (2017) β-Adrenergic Stimulation Induces Histone Deacetylase 5 (HDAC5) Nuclear Accumulation in Cardiomyocytes by B55α-PP2A-Mediated Dephosphorylation. J Am Heart Assoc 6
28343149   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

7

Chang CW, et al. (2013) Acute β-Adrenergic Activation Triggers Nuclear Import of Histone Deacetylase 5 and Delays Gq-induced Transcriptional Activation. J Biol Chem 288, 192-204
23161540   Curated Info

8

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

9

Taniguchi M, et al. (2012) Histone deacetylase 5 limits cocaine reward through cAMP-induced nuclear import. Neuron 73, 108-20
22243750   Curated Info

10

Greco TM, Yu F, Guise AJ, Cristea IM (2011) Nuclear import of histone deacetylase 5 by requisite nuclear localization signal phosphorylation. Mol Cell Proteomics 10, M110.004317
21081666   Curated Info

11

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

12

Ha CH, et al. (2010) PKA phosphorylates histone deacetylase 5 and prevents its nuclear export, leading to the inhibition of gene transcription and cardiomyocyte hypertrophy. Proc Natl Acad Sci U S A 107, 15467-72
20716686   Curated Info

13

Rikova K (2010) CST Curation Set: 9001; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

14

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info