Ser910
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Home > Phosphorylation Site Page: > Ser910  -  PRKD1 (human)

Site Information
KALGERVsIL_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447632

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 21 ) , flow cytometry ( 2 ) , immunoprecipitation ( 3 , 13 , 31 ) , mass spectrometry ( 4 , 6 , 8 , 11 , 16 , 17 , 19 , 20 ) , mutation of modification site ( 13 , 21 , 25 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 10 , 13 , 14 , 15 , 18 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ) , western blotting ( 1 , 2 , 3 , 5 , 10 , 13 , 14 , 15 , 18 , 25 , 26 , 27 , 28 , 30 , 31 )
Disease tissue studied:
breast cancer ( 6 , 26 ) , HER2 positive breast cancer ( 4 ) , luminal A breast cancer ( 4 ) , luminal B breast cancer ( 4 ) , breast cancer, surrounding tissue ( 4 ) , breast cancer, triple negative ( 4 , 6 ) , colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , leukemia ( 1 ) , acute myelogenous leukemia ( 1 ) , chronic lymphocytic leukemia ( 2 ) , liver cancer ( 14 ) , lung cancer ( 5 , 11 ) , non-small cell lung cancer ( 5 , 11 ) , lymphoma ( 1 ) , ovarian cancer ( 6 ) , pancreatic cancer ( 28 ) , pancreatic carcinoma ( 28 ) , pancreatic ductal adenocarcinoma ( 8 ) , prostate cancer ( 18 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
PLCE1 (human) ( 3 )
Putative in vivo kinases:
PRKD1 (human) ( 1 , 13 , 21 , 23 , 26 , 30 )
Kinases, in vitro:
PRKD1 (human) ( 21 , 29 )
Treatments:
AFN700 ( 1 ) , antibody ( 2 , 31 ) , arachidonic_acid ( 26 ) , ATP ( 21 ) , BAA473 ( 1 ) , bisindolylmaleimide ( 28 ) , bombesin ( 18 ) , bryostatin_1 ( 18 ) , carbachol ( 10 ) , CGP044892 ( 1 ) , CID755673 ( 2 ) , CRT0066101 ( 1 ) , endothelin ( 13 ) , fibronectin ( 22 ) , genistein ( 28 ) , GF109203X ( 21 ) , Go_6976 ( 10 , 14 , 15 , 18 , 21 , 26 ) , Go_6983 ( 14 , 15 , 26 ) , gramicidin ( 1 ) , H2O2 ( 25 ) , ischemia ( 6 ) , isoproterenol ( 13 ) , kb_NB_142-70 ( 5 ) , LPA ( 3 , 17 ) , MCC950 ( 1 ) , MSU crystals ( 1 ) , neurotensin ( 18 , 28 ) , niclosamide ( 1 ) , nigericin ( 1 ) , norepinephrine ( 13 , 21 ) , PD98059 ( 28 ) , phenylephrine ( 13 ) , phorbol_ester ( 2 , 5 , 10 , 13 , 15 , 21 , 27 , 30 , 31 ) , polyHEMA ( 22 ) , rapamycin ( 28 ) , resveratrol ( 25 ) , salicylate ( 27 ) , Salmonella typhimurium PrgI protein ( 1 ) , SB203580 ( 18 ) , serum ( 15 ) , siRNA ( 10 ) , thrombin ( 21 ) , virus infection ( 14 ) , wortmannin ( 28 )

Downstream Regulation
Effects of modification on PRKD1:
enzymatic activity, induced ( 14 , 23 ) , enzymatic activity, inhibited ( 21 ) , phosphorylation ( 21 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 )

References 

1

Heiser D, et al. (2021) Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation. PLoS One 16, e0248668
34767572   Curated Info

2

Saint-Georges S, et al. (2016) Protein kinase D-dependent CXCR4 down-regulation upon BCR triggering is linked to lymphadenopathy in chronic lymphocytic leukaemia. Oncotarget 7, 41031-41046
27127886   Curated Info

3

Wakita M, et al. (2016) Phospholipase Cϵ Activates Nuclear Factor-κB Signaling by Causing Cytoplasmic Localization of Ribosomal S6 Kinase and Facilitating Its Phosphorylation of Inhibitor κB in Colon Epithelial Cells. J Biol Chem 291, 12586-600
27053111   Curated Info

4

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

5

Ni Y, et al. (2015) PKD1 is downregulated in non-small cell lung cancer and mediates the feedback inhibition of mTORC1-S6K1 axis in response to phorbol ester. Int J Biochem Cell Biol 60, 34-42
25578563   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Nichols CB, et al. (2014) β-Adrenergic Signaling Inhibits Gq-Dependent Protein Kinase D Activation by Preventing Protein Kinase D Translocation. Circ Res 114, 1398-409
24643961   Curated Info

8

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Gehart H, et al. (2012) The BAR Domain Protein Arfaptin-1 Controls Secretory Granule Biogenesis at the trans-Golgi Network. Dev Cell 23, 756-68
22981988   Curated Info

11

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

12

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

13

Phan D, Stratton MS, Huynh QK, McKinsey TA (2011) A novel protein kinase C target site in protein kinase D is phosphorylated in response to signals for cardiac hypertrophy. Biochem Biophys Res Commun 411, 335-41
21726532   Curated Info

14

Amako Y, Syed GH, Siddiqui A (2011) Protein Kinase D Negatively Regulates Hepatitis C Virus Secretion through Phosphorylation of Oxysterol-binding Protein and Ceramide Transfer Protein. J Biol Chem 286, 11265-74
21285358   Curated Info

15

Ziegler S, et al. (2011) A novel protein kinase D phosphorylation site in the tumor suppressor Rab interactor 1 is critical for coordination of cell migration. Mol Biol Cell 22, 570-80
21209314   Curated Info

16

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

17

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

18

Hassan S, et al. (2009) Heat shock protein 27 mediates repression of androgen receptor function by protein kinase D1 in prostate cancer cells. Oncogene 28, 4386-96
19767773   Curated Info

19

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

20

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

21

Rybin VO, Guo J, Steinberg SF (2009) Protein kinase D1 autophosphorylation via distinct mechanisms at Ser744/Ser748 and Ser916. J Biol Chem 284, 2332-43
19029298   Curated Info

22

Biliran H, et al. (2008) Protein kinase D is a positive regulator of Bit1 apoptotic function. J Biol Chem 283, 28029-37
18703509   Curated Info

23

Hinchliffe KA, Irvine RF (2006) Regulation of type II PIP kinase by PKD phosphorylation. Cell Signal 18, 1906-13
16563698   Curated Info

24

Zhang W, Zheng S, Storz P, Min W (2005) Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor. J Biol Chem 280, 19036-44
15755722   Curated Info

25

Storz P, Döppler H, Toker A (2004) Activation loop phosphorylation controls protein kinase D-dependent activation of nuclear factor kappaB. Mol Pharmacol 66, 870-9
15226414   Curated Info

26

Kennett SB, Roberts JD, Olden K (2004) Requirement of protein kinase C micro activation and calpain-mediated proteolysis for arachidonic acid-stimulated adhesion of MDA-MB-435 human mammary carcinoma cells to collagen type IV. J Biol Chem 279, 3300-7
14607845   Curated Info

27

Jiang G, et al. (2003) Salicylic acid reverses phorbol 12-myristate-13-acetate (PMA)- and tumor necrosis factor alpha (TNFalpha)-induced insulin receptor substrate 1 (IRS1) serine 307 phosphorylation and insulin resistance in human embryonic kidney 293 (HEK293) cells. J Biol Chem 278, 180-6
12409308   Curated Info

28

Guha S, Rey O, Rozengurt E (2002) Neurotensin induces protein kinase C-dependent protein kinase D activation and DNA synthesis in human pancreatic carcinoma cell line PANC-1. Cancer Res 62, 1632-40
11912133   Curated Info

29

Brändlin I, et al. (2002) Protein kinase C (PKC)eta-mediated PKC mu activation modulates ERK and JNK signal pathways. J Biol Chem 277, 6490-6
11741879   Curated Info

30

Hurd C, Rozengurt E (2001) Protein kinase D is sufficient to suppress EGF-induced c-Jun Ser 63 phosphorylation. Biochem Biophys Res Commun 282, 404-8
11401472   Curated Info

31

Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274, 26543-9
10473617   Curated Info