Ser748
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Home > Phosphorylation Site Page: > Ser748  -  PRKD1 (mouse)

Site Information
GEksFRRsVVGtPAy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448590

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 40 ) , mass spectrometry ( 5 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 31 , 32 , 33 , 34 , 38 , 46 ) , mutation of modification site ( 40 , 41 , 44 , 46 , 47 ) , phospho-antibody ( 1 , 3 , 4 , 7 , 15 , 16 , 29 , 35 , 36 , 37 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ) , western blotting ( 1 , 4 , 7 , 15 , 16 , 29 , 35 , 36 , 37 , 39 , 40 , 43 , 44 , 45 )
Disease tissue studied:
anthrax infection ( 20 ) , leukemia ( 13 ) , acute myelogenous leukemia ( 13 ) , melanoma skin cancer ( 32 ) , testicular cancer ( 22 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 9 ) , 'brain, cerebellum' ( 34 ) , 'brain, cerebral cortex' ( 34 ) , 'brain, embryonic' ( 17 , 23 , 27 ) , 'brain, hippocampus, dentate gyrus' ( 34 ) , 'brain, midbrain' ( 34 ) , 'muscle, smooth' ( 1 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 42 ) , 3T3 (fibroblast) ( 36 ) , A20 (B lymphocyte) ( 41 ) , aorta ( 1 ) , blood ( 13 ) , brain ( 14 , 26 , 28 ) , cardiac ( 35 ) , COS (fibroblast) ( 15 , 43 , 44 , 47 ) , DO11 (T lymphocyte) ( 40 ) , heart ( 7 , 10 , 21 ) , HEK293T (epithelial) ( 16 , 40 , 46 ) , Hepa 1-6 (epithelial) ( 33 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 5 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 5 ) , HL-1 (myocyte) ( 5 ) , kidney ( 21 ) , liver ( 8 , 12 , 21 , 24 , 25 , 38 ) , liver [leptin (mouse), homozygous knockout] ( 12 ) , lung ( 21 ) , macrophage-peritoneum ( 11 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 18 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 19 ) , MEF (fibroblast) ( 4 , 16 , 19 , 39 ) , myocyte-heart ( 37 ) , P19 (testicular) ( 22 ) , pancreas ( 3 , 21 ) , RAW 267.4 (macrophage) ( 31 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 32 ) , spleen ( 20 ) , Swiss 3T3 (fibroblast) ( 15 , 29 , 43 , 45 ) , testis ( 21 )

Upstream Regulation
Regulatory protein:
EDG4 (human) ( 1 ) , G-alpha 11 (mouse) ( 15 ) , G-alpha(q) (mouse) ( 15 ) , LPAR1 (human) ( 1 ) , PKCD (mouse) ( 4 ) , PTPRA (mouse) ( 39 )
Putative in vivo kinases:
PKCE (mouse) ( 35 )
Kinases, in vitro:
P38D (human) ( 30 ) , PKCE (mouse) ( 44 ) , PKCH (mouse) ( 44 ) , PKCZ (mouse) ( 44 ) , PRKD1 (mouse) ( 29 , 44 , 46 )
Treatments:
AlF4- ( 15 ) , antibody ( 40 , 41 ) , autocamtide_inhibitory_peptide ( 7 ) , bombesin ( 29 , 45 ) , BSO ( 43 ) , endothelin ( 37 ) , GF109203X ( 15 , 29 , 35 ) , Go_6976 ( 35 ) , Go_6983 ( 29 ) , H2O2 ( 4 , 35 , 39 , 43 ) , IFN-gamma ( 31 ) , insulin ( 9 ) , ionomycin ( 40 ) , isoproterenol ( 37 ) , K252a ( 29 ) , linoleic_acid ( 42 ) , LPA ( 1 ) , LY294002 ( 9 , 35 ) , MitoQ ( 3 ) , NAC ( 43 ) , norepinephrine ( 35 ) , Pasteurella_multocida_toxin ( 15 ) , PDGF ( 29 ) , phorbol_ester ( 15 , 29 , 35 , 39 , 40 , 41 , 43 , 44 , 45 ) , thrombin ( 16 , 39 ) , vasopressin ( 29 )

Downstream Regulation
Effects of modification on PRKD1:
activity, induced ( 1 ) , enzymatic activity, induced ( 40 , 41 , 43 , 44 , 46 , 47 )

References 

1

Hao F, et al. (2021) LPA-mediated PKD2 activation promotes LPA-induced tissue factor expression via the p38α and JNK2 MAPK pathways in smooth muscle cells. J Biol Chem, 101152
34478715   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Liou GY, et al. (2016) Mutant KRas-Induced Mitochondrial Oxidative Stress in Acinar Cells Upregulates EGFR Signaling to Drive Formation of Pancreatic Precancerous Lesions. Cell Rep 14, 2325-36
26947075   Curated Info

4

Zhang T, Sell P, Braun U, Leitges M (2015) PKD1 Protein Is Involved in Reactive Oxygen Species-mediated Mitochondrial Depolarization in Cooperation with Protein Kinase Cδ (PKCδ). J Biol Chem 290, 10472-85
25759386   Curated Info

5

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Chakraborty A, et al. (2014) Inhibition of CaMKII Does Not Attenuate Cardiac Hypertrophy in Mice with Dysfunctional Ryanodine Receptor. PLoS One 9, e104338
25093823   Curated Info

8

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

9

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

10

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

11

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

12

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

13

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

14

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

15

Waldron RT, et al. (2012) Differential PKC-dependent and -independent PKD activation by G protein α subunits of the Gq family: selective stimulation of PKD Ser⁷⁴⁸ autophosphorylation by Gαq. Cell Signal 24, 914-21
22227248   Curated Info

16

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

17

Guo A (2011) CST Curation Set: 12476; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

18

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

19

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

20

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

21

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

22

Zhou J (2010) CST Curation Set: 9411; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

23

Zhou J (2010) CST Curation Set: 9272; Year: 2010; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)XpS
Curated Info

24

Zhou J (2009) CST Curation Set: 7446; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

25

Zhou J (2009) CST Curation Set: 7447; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

26

Zhou J (2009) CST Curation Set: 7443; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

27

Zhou J (2009) CST Curation Set: 7440; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

28

Zhou J (2009) CST Curation Set: 7442; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

29

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

30

Sumara G, et al. (2009) Regulation of PKD by the MAPK p38delta in insulin secretion and glucose homeostasis. Cell 136, 235-48
19135240   Curated Info

31

Trost M, et al. (2009) The phagosomal proteome in interferon-gamma-activated macrophages. Immunity 30, 143-54
19144319   Curated Info

32

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

33

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

34

Trinidad JC, et al. (2008) Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7, 684-96
18056256   Curated Info

35

Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8
17569658   Curated Info

36

White DP, Caswell PT, Norman JC (2007) alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration. J Cell Biol 177, 515-25
17485491   Curated Info

37

Cuello F, et al. (2007) Protein kinase D selectively targets cardiac troponin I and regulates myofilament Ca2+ sensitivity in ventricular myocytes. Circ Res 100, 864-73
17322173   Curated Info

38

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

39

Hao Q, Rutherford SA, Low B, Tang H (2006) Selective regulation of hydrogen peroxide signaling by receptor tyrosine phosphatase-alpha. Free Radic Biol Med 41, 302-10
16814111   Curated Info

40

Parra M, et al. (2005) Protein kinase D1 phosphorylates HDAC7 and induces its nuclear export after T-cell receptor activation. J Biol Chem 280, 13762-70
15623513   Curated Info

41

Wood CD, Marklund U, Cantrell DA (2005) Dual phospholipase C/diacylglycerol requirement for protein kinase D1 activation in lymphocytes. J Biol Chem 280, 6245-51
15590638   Curated Info

42

Gao Z, et al. (2004) Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol Endocrinol 18, 2024-34
15143153   Curated Info

43

Waldron RT, Rey O, Zhukova E, Rozengurt E (2004) Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D. J Biol Chem 279, 27482-93
15084589   Curated Info

44

Waldron RT, Rozengurt E (2003) Protein kinase C phosphorylates protein kinase D activation loop Ser744 and Ser748 and releases autoinhibition by the pleckstrin homology domain. J Biol Chem 278, 154-63
12407104   Curated Info

45

Zhukova E, Sinnett-Smith J, Rozengurt E (2001) Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cells. J Biol Chem 276, 40298-305
11514571   Curated Info

46

Vertommen D, et al. (2000) Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis. J Biol Chem 275, 19567-76
10867018   Curated Info

47

Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274, 26543-9
10473617   Curated Info

48

Iglesias T, Waldron RT, Rozengurt E (1998) Identification of in vivo phosphorylation sites required for protein kinase D activation. J Biol Chem 273, 27662-7
9765302   Curated Info