Ser744
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Home > Phosphorylation Site Page: > Ser744  -  PRKD1 (mouse)

Site Information
ARIIGEksFRRsVVG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448589

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 24 ) , electrophoretic mobility shift ( 15 ) , mass spectrometry ( 9 , 22 ) , mutation of modification site ( 15 , 16 , 20 , 22 , 23 , 24 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ) , phosphopeptide mapping ( 24 ) , western blotting ( 1 , 2 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 18 , 19 , 20 , 21 )
Disease tissue studied:
testicular cancer ( 9 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 2 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 17 ) , 3T3 (fibroblast) ( 12 ) , A20 (B lymphocyte) ( 16 ) , aorta ( 2 ) , cardiac ( 11 ) , COS (fibroblast) ( 7 , 18 , 20 , 23 , 24 ) , DO11 (T lymphocyte) ( 15 ) , heart ( 6 ) , HEK293T (epithelial) ( 8 , 15 , 22 ) , lymphocyte ( 1 ) , MEF (fibroblast) ( 4 , 8 , 14 , 19 ) , myocyte-heart ( 13 ) , P19 (testicular) ( 9 ) , pancreas ( 3 ) , Swiss 3T3 (fibroblast) ( 7 , 10 , 18 , 21 )

Upstream Regulation
Regulatory protein:
EDG4 (human) ( 2 ) , G-alpha 11 (mouse) ( 7 ) , G-alpha(q) (mouse) ( 7 ) , LPAR1 (human) ( 2 ) , PKCD (mouse) ( 4 ) , PTPRA (mouse) ( 14 )
Putative in vivo kinases:
PKCA (mouse) ( 19 ) , PKCE (mouse) ( 11 )
Kinases, in vitro:
PKCE (mouse) ( 20 ) , PKCH (mouse) ( 20 ) , PKCZ (mouse) ( 20 ) , PRKD1 (mouse) ( 22 )
Treatments:
AlF4- ( 7 ) , antibody ( 1 , 15 , 16 ) , autocamtide_inhibitory_peptide ( 6 ) , BMS-502 ( 1 ) , bombesin ( 10 , 21 ) , BSO ( 18 ) , endothelin ( 13 ) , GF109203X ( 7 , 10 , 11 , 18 , 19 ) , Go_6976 ( 11 ) , Go_6983 ( 10 , 18 ) , H2O2 ( 4 , 11 , 14 , 18 ) , ionomycin ( 15 ) , isoproterenol ( 13 ) , K252a ( 10 ) , linoleic_acid ( 17 ) , LPA ( 2 , 19 ) , LY294002 ( 11 ) , MitoQ ( 3 ) , NAC ( 18 ) , norepinephrine ( 11 ) , Pasteurella_multocida_toxin ( 7 ) , PDGF ( 10 ) , phorbol_ester ( 7 , 10 , 11 , 14 , 15 , 16 , 18 , 20 , 21 , 24 ) , PP2 ( 18 ) , PTX ( 19 ) , Ro31-8220 ( 19 ) , thrombin ( 8 , 14 ) , vasopressin ( 10 )

Downstream Regulation
Effects of modification on PRKD1:
activity, induced ( 2 ) , enzymatic activity, induced ( 15 , 16 , 18 , 20 , 22 , 23 , 24 )

References 

1

Wichroski M, et al. (2023) DGKα/ζ inhibitors combine with PD-1 checkpoint therapy to promote T cell-mediated antitumor immunity. Sci Transl Med 15, eadh1892
37878674   Curated Info

2

Hao F, et al. (2021) LPA-mediated PKD2 activation promotes LPA-induced tissue factor expression via the p38α and JNK2 MAPK pathways in smooth muscle cells. J Biol Chem, 101152
34478715   Curated Info

3

Liou GY, et al. (2016) Mutant KRas-Induced Mitochondrial Oxidative Stress in Acinar Cells Upregulates EGFR Signaling to Drive Formation of Pancreatic Precancerous Lesions. Cell Rep 14, 2325-36
26947075   Curated Info

4

Zhang T, Sell P, Braun U, Leitges M (2015) PKD1 Protein Is Involved in Reactive Oxygen Species-mediated Mitochondrial Depolarization in Cooperation with Protein Kinase Cδ (PKCδ). J Biol Chem 290, 10472-85
25759386   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Chakraborty A, et al. (2014) Inhibition of CaMKII Does Not Attenuate Cardiac Hypertrophy in Mice with Dysfunctional Ryanodine Receptor. PLoS One 9, e104338
25093823   Curated Info

7

Waldron RT, et al. (2012) Differential PKC-dependent and -independent PKD activation by G protein α subunits of the Gq family: selective stimulation of PKD Ser⁷⁴⁸ autophosphorylation by Gαq. Cell Signal 24, 914-21
22227248   Curated Info

8

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

9

Zhou J (2010) CST Curation Set: 9411; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

10

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

11

Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8
17569658   Curated Info

12

White DP, Caswell PT, Norman JC (2007) alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration. J Cell Biol 177, 515-25
17485491   Curated Info

13

Cuello F, et al. (2007) Protein kinase D selectively targets cardiac troponin I and regulates myofilament Ca2+ sensitivity in ventricular myocytes. Circ Res 100, 864-73
17322173   Curated Info

14

Hao Q, Rutherford SA, Low B, Tang H (2006) Selective regulation of hydrogen peroxide signaling by receptor tyrosine phosphatase-alpha. Free Radic Biol Med 41, 302-10
16814111   Curated Info

15

Parra M, et al. (2005) Protein kinase D1 phosphorylates HDAC7 and induces its nuclear export after T-cell receptor activation. J Biol Chem 280, 13762-70
15623513   Curated Info

16

Wood CD, Marklund U, Cantrell DA (2005) Dual phospholipase C/diacylglycerol requirement for protein kinase D1 activation in lymphocytes. J Biol Chem 280, 6245-51
15590638   Curated Info

17

Gao Z, et al. (2004) Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol Endocrinol 18, 2024-34
15143153   Curated Info

18

Waldron RT, Rey O, Zhukova E, Rozengurt E (2004) Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D. J Biol Chem 279, 27482-93
15084589   Curated Info

19

Yuan J, Slice LW, Gu J, Rozengurt E (2003) Cooperation of Gq, Gi, and G12/13 in protein kinase D activation and phosphorylation induced by lysophosphatidic acid. J Biol Chem 278, 4882-91
12477719   Curated Info

20

Waldron RT, Rozengurt E (2003) Protein kinase C phosphorylates protein kinase D activation loop Ser744 and Ser748 and releases autoinhibition by the pleckstrin homology domain. J Biol Chem 278, 154-63
12407104   Curated Info

21

Zhukova E, Sinnett-Smith J, Rozengurt E (2001) Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cells. J Biol Chem 276, 40298-305
11514571   Curated Info

22

Vertommen D, et al. (2000) Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis. J Biol Chem 275, 19567-76
10867018   Curated Info

23

Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274, 26543-9
10473617   Curated Info

24

Iglesias T, Waldron RT, Rozengurt E (1998) Identification of in vivo phosphorylation sites required for protein kinase D activation. J Biol Chem 273, 27662-7
9765302   Curated Info