Ser540
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Home > Phosphorylation Site Page: > Ser540  -  SIRT1 (human)

Site Information
HVsEDsssPERtsPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4770118

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 1 , 7 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 7 ) , western blotting ( 1 , 7 )
Disease tissue studied:
bone cancer ( 1 ) , gastric cancer ( 2 ) , lung cancer ( 4 , 5 ) , non-small cell lung cancer ( 4 , 5 ) , non-small cell lung adenocarcinoma ( 5 ) , non-small cell large cell lung carcinoma ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CDK1 (human) ( 1 )
Kinases, in vitro:
CDK1 (human) ( 7 )

Downstream Regulation
Effects of modification on SIRT1:
acetylation ( 7 ) , enzymatic activity, induced ( 7 ) , intracellular localization ( 1 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 7 ) , cytoskeletal reorganization ( 1 )

References 

1

Ling H, et al. (2018) Histone Deacetylase SIRT1 Targets Plk2 to Regulate Centriole Duplication. Cell Rep 25, 2851-2865.e3
30517871   Curated Info

2

Rikova K (2012) CST Curation Set: 16141; Year: 2012; Biosample/Treatment: cell line, MKN45/crizotinib; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST](AGC)
Curated Info

3

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

4

Rikova K (2011) CST Curation Set: 10893; Year: 2011; Biosample/Treatment: cell line, NCI-H460/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

5

Rikova K (2011) CST Curation Set: 10885; Year: 2011; Biosample/Treatment: cell line, NCI-H2342/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

6

Rikova K (2010) CST Curation Set: 10041; Year: 2010; Biosample/Treatment: cell line, DFCI ERC17/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

7

Sasaki T, et al. (2008) Phosphorylation regulates SIRT1 function. PLoS One 3, e4020
19107194   Curated Info