Ser191
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser191  -  CTNNB1 (human)

Site Information
sRHAIMRsPQMVsAI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 467710

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 5 , 6 ) , mass spectrometry ( 2 , 3 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 15 , 16 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 , 5 , 6 ) , phospho-antibody ( 6 ) , western blotting ( 5 , 6 )
Disease tissue studied:
breast cancer ( 9 , 16 ) , breast ductal carcinoma ( 9 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 9 ) , hepatocellular carcinoma, surrounding tissue ( 23 ) , lung cancer ( 1 , 12 , 16 , 25 ) , non-small cell lung cancer ( 1 , 16 , 25 ) , non-small cell lung adenocarcinoma ( 1 , 12 ) , neuroblastoma ( 15 ) , ovarian cancer ( 9 ) , melanoma skin cancer ( 8 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 24 ) , 293 (epithelial) [ADRB1 (human), no information, overexpresses human beta1-adrenergic (ß1AR- HEK293)] ( 27 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 21 ) , 293 (epithelial) ( 5 ) , 786-O (renal) [VHL (human), transfection] ( 7 ) , 786-O (renal) ( 7 ) , A498 (renal) ( 22 ) , A549 (pulmonary) ( 1 , 13 ) , breast ( 3 , 9 ) , BT-20 (breast cell) ( 16 ) , BT-549 (breast cell) ( 16 ) , Calu 6 (pulmonary) ( 16 ) , COS (fibroblast) ( 28 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 17 ) , Flp-In T-Rex-293 (epithelial) ( 17 ) , H2009 (pulmonary) ( 16 ) , H2077 (pulmonary) ( 16 ) , H2887 (pulmonary) ( 16 ) , H322M (pulmonary) ( 16 ) , HCC1937 (breast cell) ( 16 ) , HCC2279 (pulmonary) ( 16 ) , HEK293T (epithelial) ( 6 ) , HeLa (cervical) ( 20 ) , hepatocyte-liver ( 23 ) , HOP62 (pulmonary) ( 16 ) , HUES-9 ('stem, embryonic') ( 19 ) , liver ( 11 ) , lung ( 12 ) , MC3T3-E1 (preosteoblast) ( 5 ) , MCF-7 (breast cell) ( 16 ) , MDA-MB-231 (breast cell) ( 16 ) , MDA-MB-468 (breast cell) ( 16 ) , NB10 (neural crest) ( 15 ) , NCI-H1299 (pulmonary) ( 25 ) , NCI-H1395 (pulmonary) ( 16 ) , NCI-H157 (pulmonary) ( 16 ) , NCI-H1648 (pulmonary) ( 16 ) , NCI-H1666 (pulmonary) ( 16 ) , NCI-H2030 (pulmonary) ( 16 ) , NCI-H2172 (pulmonary) ( 16 ) , NCI-H322 (pulmonary) ( 16 ) , NCI-H520 (squamous) ( 16 ) , NPC (neural crest) ( 15 ) , ovary ( 9 ) , PC9 (pulmonary) ( 16 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 10 ) , SH-SY5Y (neural crest) ( 10 ) , TERT20 ('stem, mesenchymal') ( 26 ) , Vero E6-S ('epithelial, kidney') ( 2 ) , WM239A (melanocyte) ( 8 )

Upstream Regulation
Kinases, in vitro:
CDK5 (human) ( 29 )
Treatments:
LRRK2-IN-1 ( 10 )

Downstream Regulation
Effects of modification on CTNNB1:
intracellular localization ( 5 ) , molecular association, regulation ( 5 ) , protein conformation ( 5 ) , protein stabilization ( 5 )
Effects of modification on biological processes:
transcription, induced ( 5 )
Induce interaction with:
PIN1 (human) ( 5 )

References 

1

Kim DE, et al. (2023) PLK1-mediated phosphorylation of β-catenin enhances its stability and transcriptional activity for extracellular matrix remodeling in metastatic NSCLC. Theranostics 13, 1198-1216
36793862   Curated Info

2

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182
32645325   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Shin HR, et al. (2016) Pin1-mediated Modification Prolongs the Nuclear Retention of β-Catenin in Wnt3a-induced Osteoblast Differentiation. J Biol Chem 291, 5555-65
26740630   Curated Info

6

Yin N, et al. (2016) p38γ MAPK is required for inflammation-associated colon tumorigenesis. Oncogene 35, 1039-48
25961922   Curated Info

7

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
26506913   Curated Info

8

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

9

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

10

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

11

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

12

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

13

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

14

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

15

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

16

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

17

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

18

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

19

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

20

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

21

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

22

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

23

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

24

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

25

Tsai CF, et al. (2008) Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics. J Proteome Res 7, 4058-69
18707149   Curated Info

26

Thingholm TE, et al. (2008) TiO2-Based Phosphoproteomic Analysis of the Plasma Membrane and the Effects of Phosphatase Inhibitor Treatment. J Proteome Res 7, 3304-3313
18578522   Curated Info

27

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

28

Wang Y, et al. (2007) Profiling signaling polarity in chemotactic cells. Proc Natl Acad Sci U S A 104, 8328-33
17494752   Curated Info

29

Muñoz JP, Huichalaf CH, Orellana D, Maccioni RB (2007) cdk5 modulates beta- and delta-catenin/Pin1 interactions in neuronal cells. J Cell Biochem 100, 738-49
17009320   Curated Info