Ser619

Site Information
RNNLRPKsKsEyELS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4754726

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 4 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 2 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 2 ) , HL-1 (myocyte) ( 2 ) , kidney ( 4 ) , lung ( 4 ) , macrophage-bone marrow ( 5 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 5 ) , MC3T3-E1 (preosteoblast) ( 1 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 3 ) , spleen ( 4 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 5 )

Relevant cell line - cell type - tissue:

'fat, brown' ( 4 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 2 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 2 ) , HL-1 (myocyte) ( 2 ) , kidney ( 4 ) , lung ( 4 ) , macrophage-bone marrow ( 5 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 5 ) , MC3T3-E1 (preosteoblast) ( 1 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 3 ) , spleen ( 4 )

Upstream Regulation
Treatments:	LPS ( 5 ) , PTH(1-34) ( 1 )

References
1	Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50 26160508 Curated Info
2	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
3	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
4	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
5	Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371 20531401 Curated Info