Ser16

Site Information
GQSAAVAsPGGsIDs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4753769

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 5 ) , C2C12 (myoblast) ( 7 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 10 ) , lung ( 10 ) , macrophage-peritoneum ( 6 ) , MC3T3-E1 (preosteoblast) ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 8 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 9 ) , pancreas ( 10 ) , RAW 264.7 (macrophage) ( 2 ) , spleen ( 10 ) , stromal ( 4 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 5 ) , C2C12 (myoblast) ( 7 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 10 ) , lung ( 10 ) , macrophage-peritoneum ( 6 ) , MC3T3-E1 (preosteoblast) ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 8 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 9 ) , pancreas ( 10 ) , RAW 264.7 (macrophage) ( 2 ) , spleen ( 10 ) , stromal ( 4 )

Upstream Regulation
Treatments:	insulin ( 5 ) , ischemia ( 4 ) , SB202190 ( 7 )

References
1	Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50 26160508 Curated Info
2	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
3	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
6	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
7	Knight JD, et al. (2012) A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts. Skelet Muscle 2, 5 22394512 Curated Info
8	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
9	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
10	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info