Ser266
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser266  -  HDAC4 (human)

Site Information
KVAERRssPLLRRKD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4737976

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 7 ) , mass spectrometry ( 1 , 2 , 5 , 6 , 8 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 2 , 3 , 7 ) , phospho-antibody ( 7 ) , western blotting ( 2 , 7 )
Disease tissue studied:
cervical cancer ( 13 ) , cervical adenocarcinoma ( 13 ) , leukemia ( 2 ) , chronic myelogenous leukemia ( 2 ) , lung cancer ( 12 ) , non-small cell lung cancer ( 12 ) , pancreatic ductal adenocarcinoma ( 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 7 ) , 'pancreatic, ductal'-pancreas ( 6 ) , C2C12 (myoblast) ( 2 ) , HEK293T (epithelial) ( 2 ) , HeLa (cervical) ( 5 , 10 , 11 ) , HeLa S3 (cervical) ( 13 ) , Jurkat (T lymphocyte) ( 8 ) , K562 (erythroid) ( 2 , 10 ) , lung ( 12 ) , Schwann ( 3 ) , Vero E6-S ('epithelial, kidney') ( 1 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 2 , 7 )
Kinases, in vitro:
PKACA (human) ( 2 )
Treatments:
cAMP_analog ( 2 , 7 ) , H-89 ( 2 ) , isoproterenol ( 7 ) , N6-benzoyl-cAMP ( 7 ) , NKH_477 ( 2 ) , nocodazole ( 13 ) , propranolol ( 7 ) , Rp-cAMPS ( 7 ) , siRNA ( 2 )

Downstream Regulation
Effects of modification on HDAC4:
activity, induced ( 2 ) , intracellular localization ( 3 , 7 )
Effects of modification on biological processes:
transcription, inhibited ( 2 , 7 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182
32645325   Curated Info

2

Doddi SK, Kummari G, M V J, Kalle AM (2019) Protein kinase A-mediated novel Serine 584 phosphorylation of HDAC4. Biochem Cell Biol
30661366   Curated Info

3

Gomis-Coloma C, et al. (2018) Class IIa histone deacetylases link cAMP signaling to the myelin transcriptional program of Schwann cells. J Cell Biol
29472387   Curated Info

4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

7

Liu Y, Schneider MF (2013) Opposing HDAC4 nuclear fluxes due to phosphorylation by β-adrenergic activated protein kinase A or by activity or Epac activated CaMKII in skeletal muscle fibres. J Physiol 591, 3605-23
23652597   Curated Info

8

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

11

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

12

Rikova K (2010) CST Curation Set: 9001; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info