Thr58

Site Information
KKFELLPtPPLsPsR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448496

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 8 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 7 , 9 , 10 , 11 , 12 , 13 , 16 ) , mutation of modification site ( 2 , 6 , 20 , 21 ) , phospho-antibody ( 8 , 14 , 15 , 17 , 19 , 20 , 21 ) , western blotting ( 8 , 14 , 15 , 17 , 19 , 21 )
Disease tissue studied:	anthrax infection ( 11 ) , breast cancer ( 6 ) , colorectal cancer ( 17 ) , colorectal carcinoma ( 17 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 5 ) , 293 (epithelial) ( 15 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 13 ) , 32Dcl3 (myeloid) ( 13 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , BJ (fibroblast) [NBS1 (human)] ( 20 ) , breast cell [Myc (mouse), transgenic] ( 6 ) , embryo ( 8 ) , ESC ( 14 ) , HCT116 (intestinal) ( 17 ) , heart ( 15 ) , HEK293-A (epithelial) ( 20 ) , Hepa 1-6 (epithelial) ( 16 ) , JB6 CI41 (epidermal) ( 21 ) , lung ( 12 ) , macrophage-peritoneum ( 7 ) , MEF (fibroblast) [IGF1R (mouse)] ( 20 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 10 ) , MEF (fibroblast) ( 10 , 19 ) , myocyte-heart ( 15 ) , RAW 264.7 (macrophage) ( 3 ) , REF52 (fibroblast) ( 20 ) , spleen ( 11 , 12 ) , T lymphocyte-spleen ( 2 )

Upstream Regulation
Regulatory protein:	ERK2 (rat) ( 15 ) , FBXW7 iso3 (mouse) ( 8 ) , FLT3 (mouse) ( 13 ) , GLMN (mouse) ( 8 ) , GSK3A (mouse) ( 14 ) , GSK3B (mouse) ( 14 ) , P38A (mouse) ( 21 ) , PKCE (mouse) ( 18 ) , TSC2 (mouse) ( 19 )
Putative in vivo kinases:	GSK3B (mouse) ( 14 )
Treatments:	4-HT ( 14 ) , BIO ( 14 ) , EGF ( 21 ) , IL-2 ( 2 ) , IL-33 ( 3 ) , LIF ( 14 ) , LY294002 ( 14 ) , rapamycin ( 19 ) , UV ( 18 )

Downstream Regulation
Effects of modification on Myc:	molecular association, regulation ( 20 ) , protein degradation ( 2 , 8 , 14 , 20 )
Effects of modification on biological processes:	cell differentiation, altered ( 14 )
Induce interaction with:	PIN1 (human) ( 20 )

References
1	Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 28852199 Curated Info
2	Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24 26136212 Curated Info
3	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
6	Hollern DP, Yuwanita I, Andrechek ER (2013) A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene 32, 1296-304 22525269 Curated Info
7	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
8	Tron AE, et al. (2012) The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell 46, 67-78 22405651 Curated Info
9	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
10	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
11	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
12	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
13	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
14	Bechard M, Dalton S (2009) Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol 29, 2092-104 19223464 Curated Info
15	Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83 19060905 Curated Info
16	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info
17	Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19 18438430 Curated Info
18	Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94 17283176 Curated Info
19	Zhang HH, et al. (2006) S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Mol Cell 24, 185-97 17052453 Curated Info
20	Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18 15048125 Curated Info
21	He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42 12748197 Curated Info