Thr58
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Home > Phosphorylation Site Page: > Thr58  -  Myc (mouse)

Site Information
KKFELLPtPPLsPsR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448496

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 7 , 9 , 10 , 11 , 12 , 13 , 16 ) , mutation of modification site ( 2 , 6 , 20 , 21 ) , phospho-antibody ( 8 , 14 , 15 , 17 , 19 , 20 , 21 ) , western blotting ( 8 , 14 , 15 , 17 , 19 , 21 )
Disease tissue studied:
anthrax infection ( 11 ) , breast cancer ( 6 ) , colorectal cancer ( 17 ) , colorectal carcinoma ( 17 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ERK2 (rat) ( 15 ) , FBXW7 iso3 (mouse) ( 8 ) , FLT3 (mouse) ( 13 ) , GLMN (mouse) ( 8 ) , GSK3A (mouse) ( 14 ) , GSK3B (mouse) ( 14 ) , P38A (mouse) ( 21 ) , PKCE (mouse) ( 18 ) , TSC2 (mouse) ( 19 )
Putative in vivo kinases:
GSK3B (mouse) ( 14 )
Treatments:
4-HT ( 14 ) , BIO ( 14 ) , EGF ( 21 ) , IL-2 ( 2 ) , IL-33 ( 3 ) , LIF ( 14 ) , LY294002 ( 14 ) , rapamycin ( 19 ) , UV ( 18 )

Downstream Regulation
Effects of modification on Myc:
molecular association, regulation ( 20 ) , protein degradation ( 2 , 8 , 14 , 20 )
Effects of modification on biological processes:
cell differentiation, altered ( 14 )
Induce interaction with:
PIN1 (human) ( 20 )

References 

1

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

2

Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24
26136212   Curated Info

3

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Hollern DP, Yuwanita I, Andrechek ER (2013) A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene 32, 1296-304
22525269   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Tron AE, et al. (2012) The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell 46, 67-78
22405651   Curated Info

9

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

10

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

11

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

12

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

13

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

14

Bechard M, Dalton S (2009) Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol 29, 2092-104
19223464   Curated Info

15

Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83
19060905   Curated Info

16

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

17

Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19
18438430   Curated Info

18

Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94
17283176   Curated Info

19

Zhang HH, et al. (2006) S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Mol Cell 24, 185-97
17052453   Curated Info

20

Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18
15048125   Curated Info

21

He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42
12748197   Curated Info