Thr73
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Home > Phosphorylation Site Page: > Thr73  -  Myc (mouse)

Site Information
KKFELLPtPPLsPsR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448496

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 12 ) , mass spectrometry ( 5 , 6 , 7 , 9 , 11 , 13 , 14 , 15 , 16 , 17 , 20 ) , mutation of modification site ( 1 , 3 , 4 , 6 , 10 , 24 , 25 ) , phospho-antibody ( 2 , 12 , 18 , 19 , 21 , 23 , 24 , 25 ) , western blotting ( 2 , 12 , 18 , 19 , 21 , 23 , 25 )
Disease tissue studied:
anthrax infection ( 15 ) , breast cancer ( 10 ) , colorectal cancer ( 21 ) , colorectal carcinoma ( 21 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell squamous cell lung carcinoma ( 1 ) , small-cell lung cancer ( 1 ) , lymphoma ( 3 ) , B cell lymphoma ( 3 ) , non-Hodgkin's lymphoma ( 3 ) , follicular lymphoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CEP55 (human) ( 2 ) , ERK2 (rat) ( 19 ) , FBXW7 iso3 (mouse) ( 12 ) , FLT3 (mouse) ( 17 ) , GLMN (mouse) ( 12 ) , GSK3A (mouse) ( 18 ) , GSK3B (mouse) ( 18 ) , P38A (mouse) ( 25 ) , PKCE (mouse) ( 22 ) , TSC2 (mouse) ( 23 )
Putative in vivo kinases:
GSK3B (mouse) ( 2 , 18 )
Treatments:
4-HT ( 18 ) , BIO ( 18 ) , CHIR99021 ( 2 ) , EGF ( 25 ) , IL-2 ( 6 ) , IL-33 ( 7 ) , LIF ( 18 ) , LY294002 ( 18 ) , rapamycin ( 23 ) , UV ( 22 )

Downstream Regulation
Effects of modification on Myc:
molecular association, regulation ( 24 ) , phosphorylation ( 4 ) , protein degradation ( 1 , 2 , 4 , 6 , 12 , 18 , 24 )
Effects of modification on biological processes:
apoptosis, induced ( 2 , 3 , 4 ) , carcinogenesis, inhibited ( 1 , 4 ) , cell differentiation, altered ( 18 ) , cell growth, inhibited ( 1 , 2 , 3 , 4 ) , transcription, inhibited ( 3 , 4 )
Induce interaction with:
PIN1 (human) ( 24 )

Disease / Diagnostics Relevance
Relevant diseases:
T cell lymphoma ( 1 )

References 

1

Daniel CJ, et al. (2022) T-cell dysfunction upon expression of MYC with altered phosphorylation at Threonine 58 and Serine 62. Mol Cancer Res
35380701   Curated Info

2

Rashidieh B, et al. (2021) Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis. PLoS Genet 17, e1009334
34710087   Curated Info

3

Szydłowski M, et al. (2021) Inhibition of PIM Kinases in DLBCL Targets MYC Transcriptional Program and Augments the Efficacy of Anti-CD20 Antibodies. Cancer Res
34625423   Curated Info

4

Wang X, et al. (2020) Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma. Oncogenesis
32895364   Curated Info

5

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32
28852199   Curated Info

6

Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24
26136212   Curated Info

7

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

8

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

9

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

10

Hollern DP, Yuwanita I, Andrechek ER (2013) A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene 32, 1296-304
22525269   Curated Info

11

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

12

Tron AE, et al. (2012) The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell 46, 67-78
22405651   Curated Info

13

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

14

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

15

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

16

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

17

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

18

Bechard M, Dalton S (2009) Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol 29, 2092-104
19223464   Curated Info

19

Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83
19060905   Curated Info

20

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

21

Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19
18438430   Curated Info

22

Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94
17283176   Curated Info

23

Zhang HH, et al. (2006) S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Mol Cell 24, 185-97
17052453   Curated Info

24

Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18
15048125   Curated Info

25

He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42
12748197   Curated Info