Thr73

Site Information
KKFELLPtPPLsPsR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448496

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 12 ) , mass spectrometry ( 5 , 6 , 7 , 9 , 11 , 13 , 14 , 15 , 16 , 17 , 20 ) , mutation of modification site ( 1 , 3 , 4 , 6 , 10 , 24 , 25 ) , phospho-antibody ( 2 , 12 , 18 , 19 , 21 , 23 , 24 , 25 ) , western blotting ( 2 , 12 , 18 , 19 , 21 , 23 , 25 )
Disease tissue studied:	anthrax infection ( 15 ) , breast cancer ( 10 ) , colorectal cancer ( 21 ) , colorectal carcinoma ( 21 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell squamous cell lung carcinoma ( 1 ) , small-cell lung cancer ( 1 ) , lymphoma ( 3 ) , B cell lymphoma ( 3 ) , non-Hodgkin's lymphoma ( 3 ) , follicular lymphoma ( 3 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 9 ) , 293 (epithelial) ( 19 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 17 ) , 32Dcl3 (myeloid) ( 17 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 5 ) , BJ (fibroblast) [NBS1 (human)] ( 24 ) , breast cell [Myc (mouse), transgenic] ( 10 ) , embryo ( 12 ) , ESC ( 18 ) , HCT116 (intestinal) ( 21 ) , heart ( 19 ) , HEK293-A (epithelial) ( 24 ) , Hepa 1-6 (epithelial) ( 20 ) , JB6 CI41 (epidermal) ( 25 ) , lung ( 16 ) , macrophage-peritoneum ( 11 ) , MEF (fibroblast) [IGF1R (mouse)] ( 24 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 13 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 14 ) , MEF (fibroblast) ( 2 , 14 , 23 ) , myocyte-heart ( 19 ) , NCI-H128 (pulmonary) ( 1 ) , NCI-H520 (squamous) ( 1 ) , RAW 264.7 (macrophage) ( 7 ) , REF52 (fibroblast) ( 24 ) , spleen ( 15 , 16 ) , SU-DHL-4 (B lymphocyte) ( 3 ) , T lymphocyte-spleen ( 6 )

Upstream Regulation
Regulatory protein:	CEP55 (human) ( 2 ) , ERK2 (rat) ( 19 ) , FBXW7 iso3 (mouse) ( 12 ) , FLT3 (mouse) ( 17 ) , GLMN (mouse) ( 12 ) , GSK3A (mouse) ( 18 ) , GSK3B (mouse) ( 18 ) , P38A (mouse) ( 25 ) , PKCE (mouse) ( 22 ) , TSC2 (mouse) ( 23 )
Putative in vivo kinases:	GSK3B (mouse) ( 2 , 18 )
Treatments:	4-HT ( 18 ) , BIO ( 18 ) , CHIR99021 ( 2 ) , EGF ( 25 ) , IL-2 ( 6 ) , IL-33 ( 7 ) , LIF ( 18 ) , LY294002 ( 18 ) , rapamycin ( 23 ) , UV ( 22 )

Downstream Regulation
Effects of modification on Myc:	molecular association, regulation ( 24 ) , phosphorylation ( 4 ) , protein degradation ( 1 , 2 , 4 , 6 , 12 , 18 , 24 )
Effects of modification on biological processes:	apoptosis, induced ( 2 , 3 , 4 ) , carcinogenesis, inhibited ( 1 , 4 ) , cell differentiation, altered ( 18 ) , cell growth, inhibited ( 1 , 2 , 3 , 4 ) , transcription, inhibited ( 3 , 4 )
Induce interaction with:	PIN1 (human) ( 24 )

Disease / Diagnostics Relevance
Relevant diseases:	T cell lymphoma ( 1 )

References
1	Daniel CJ, et al. (2022) T-cell dysfunction upon expression of MYC with altered phosphorylation at Threonine 58 and Serine 62. Mol Cancer Res 35380701 Curated Info
2	Rashidieh B, et al. (2021) Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis. PLoS Genet 17, e1009334 34710087 Curated Info
3	Szydłowski M, et al. (2021) Inhibition of PIM Kinases in DLBCL Targets MYC Transcriptional Program and Augments the Efficacy of Anti-CD20 Antibodies. Cancer Res 34625423 Curated Info
4	Wang X, et al. (2020) Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma. Oncogenesis 32895364 Curated Info
5	Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32 28852199 Curated Info
6	Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24 26136212 Curated Info
7	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
8	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
9	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
10	Hollern DP, Yuwanita I, Andrechek ER (2013) A mouse model with T58A mutations in Myc reduces the dependence on KRas mutations and has similarities to claudin-low human breast cancer. Oncogene 32, 1296-304 22525269 Curated Info
11	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
12	Tron AE, et al. (2012) The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell 46, 67-78 22405651 Curated Info
13	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
14	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
15	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
16	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
17	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
18	Bechard M, Dalton S (2009) Subcellular localization of glycogen synthase kinase 3beta controls embryonic stem cell self-renewal. Mol Cell Biol 29, 2092-104 19223464 Curated Info
19	Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83 19060905 Curated Info
20	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info
21	Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19 18438430 Curated Info
22	Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94 17283176 Curated Info
23	Zhang HH, et al. (2006) S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Mol Cell 24, 185-97 17052453 Curated Info
24	Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18 15048125 Curated Info
25	He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42 12748197 Curated Info