Ser77

Site Information
LLPtPPLsPsRRsGL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448586

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 2 , 7 ) , immunoprecipitation ( 9 ) , mass spectrometry ( 6 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 18 ) , mutation of modification site ( 4 , 9 , 10 , 22 , 23 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 7 , 8 , 9 , 19 , 20 , 22 , 23 ) , western blotting ( 1 , 2 , 3 , 5 , 8 , 9 , 19 , 20 , 23 )
Disease tissue studied:	anthrax infection ( 17 ) , colorectal cancer ( 20 ) , colorectal carcinoma ( 20 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , non-small cell squamous cell lung carcinoma ( 4 ) , small-cell lung cancer ( 4 ) , lymphoma ( 5 ) , B cell lymphoma ( 5 ) , non-Hodgkin's lymphoma ( 5 ) , pancreatic cancer ( 3 ) , pancreatic carcinoma ( 3 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 11 ) , 'brain, cerebellum' ( 2 ) , 'brain, cerebral cortex' ( 2 ) , 293 (epithelial) ( 19 ) , BJ (fibroblast) [NBS1 (human)] ( 22 ) , D425 ( 2 ) , epidermal ( 7 ) , HCT116 (intestinal) ( 20 ) , heart ( 19 ) , HEK293-A (epithelial) ( 22 ) , HeLa (cervical) ( 9 ) , intestine ( 9 ) , JB6 CI41 (epidermal) ( 23 ) , lung ( 18 ) , lymph node ( 8 ) , lymphocyte ( 6 ) , macrophage ( 1 ) , macrophage-peritoneum ( 14 ) , MEF (fibroblast) [IGF1R (mouse)] ( 22 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 15 ) , MIA PaCa-2 (pancreatic) ( 3 ) , myocyte-heart ( 19 ) , NCI-H128 (pulmonary) ( 4 ) , NCI-H520 (squamous) ( 4 ) , NPC (neural crest) ( 2 ) , OCI-ly3 (B lymphocyte) ( 5 ) , PANC-1 (pancreatic) ( 3 ) , RAW 264.7 (macrophage) ( 12 ) , REF52 (fibroblast) ( 22 ) , spleen ( 17 , 18 ) , SU-DHL-16 (B lymphocyte) ( 5 ) , T lymphocyte-spleen ( 10 , 16 )

Upstream Regulation
Regulatory protein:	ERK2 (rat) ( 19 ) , P38A (mouse) ( 23 ) , PKCE (mouse) ( 21 )
Putative upstream phosphatases:	DULLARD (human) ( 2 )
Phosphatases, in vitro:	PPP2CA (human) ( 22 )
Treatments:	EGF ( 23 ) , IL-2 ( 10 ) , IL-33 ( 12 ) , Matrine ( 5 ) , okadaic_acid ( 22 ) , rapamycin ( 1 ) , Sepantronium ( 3 ) , serum ( 22 ) , siRNA ( 2 ) , TGF-beta ( 1 ) , UV ( 21 )

Downstream Regulation
Effects of modification on Myc:	molecular association, regulation ( 22 ) , phosphorylation ( 22 ) , protein degradation ( 4 ) , protein stabilization ( 2 , 3 , 5 , 22 )
Effects of modification on biological processes:	apoptosis, inhibited ( 2 ) , carcinogenesis, induced ( 2 , 3 ) , carcinogenesis, inhibited ( 4 ) , cell cycle regulation ( 5 ) , cell growth, induced ( 2 , 3 , 5 ) , cell growth, inhibited ( 4 ) , signaling pathway regulation ( 5 ) , transcription, altered ( 22 ) , transcription, induced ( 1 )
Induce interaction with:	PIN1 (mouse) ( 22 )

Disease / Diagnostics Relevance
Relevant diseases:	B cell lymphoma ( 4 ) , T cell lymphoma ( 4 )

References
1	Gauthier T, et al. (2023) TGF-β uncouples glycolysis and inflammation in macrophages and controls survival during sepsis. Sci Signal 16, eade0385 37552767 Curated Info
2	Luo Z, et al. (2023) Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability. Nat Commun 14, 762 36765089 Curated Info
3	Chang WH, et al. (2022) Oncogenic RAS promotes MYC protein stability by upregulating the expression of the inhibitor of apoptosis protein (IAP) family member Survivin. J Biol Chem, 102842 36581205 Curated Info
4	Daniel CJ, et al. (2022) T-cell dysfunction upon expression of MYC with altered phosphorylation at Threonine 58 and Serine 62. Mol Cancer Res 35380701 Curated Info
5	Gu J, et al. (2021) Matrine suppresses cell growth of diffuse large B-cell lymphoma via inhibiting CaMKIIγ/c-Myc/CDK6 signaling pathway. BMC Complement Med Ther 21, 163 34088288 Curated Info
6	Fish K, et al. (2020) Rewiring of B cell receptor signaling by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A 33020271 Curated Info
7	Wang X, et al. (2020) Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma. Oncogenesis 32895364 Curated Info
8	Guo H, et al. (2018) Dual inhibition of PI3K signaling and histone deacetylation halts proliferation and induces lethality in mantle cell lymphoma. Oncogene 30361685 Curated Info
9	Myant K, et al. (2015) Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation. Cell Rep 12, 1019-31 26235622 Curated Info
10	Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24 26136212 Curated Info
11	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
12	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
13	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
14	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
15	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
16	Navarro MN, et al. (2011) Phosphoproteomic analysis reveals an intrinsic pathway for the regulation of histone deacetylase 7 that controls the function of cytotoxic T lymphocytes. Nat Immunol 12, 352-61 21399638 Curated Info
17	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
18	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
19	Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83 19060905 Curated Info
20	Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19 18438430 Curated Info
21	Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94 17283176 Curated Info
22	Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18 15048125 Curated Info
23	He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42 12748197 Curated Info