Ser77
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Home > Phosphorylation Site Page: > Ser77  -  Myc (mouse)

Site Information
LLPtPPLsPsRRsGL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448586

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 , 7 ) , immunoprecipitation ( 9 ) , mass spectrometry ( 6 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 18 ) , mutation of modification site ( 4 , 9 , 10 , 22 , 23 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 7 , 8 , 9 , 19 , 20 , 22 , 23 ) , western blotting ( 1 , 2 , 3 , 5 , 8 , 9 , 19 , 20 , 23 )
Disease tissue studied:
anthrax infection ( 17 ) , colorectal cancer ( 20 ) , colorectal carcinoma ( 20 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , non-small cell squamous cell lung carcinoma ( 4 ) , small-cell lung cancer ( 4 ) , lymphoma ( 5 ) , B cell lymphoma ( 5 ) , non-Hodgkin's lymphoma ( 5 ) , pancreatic cancer ( 3 ) , pancreatic carcinoma ( 3 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 11 ) , 'brain, cerebellum' ( 2 ) , 'brain, cerebral cortex' ( 2 ) , 293 (epithelial) ( 19 ) , BJ (fibroblast) [NBS1 (human)] ( 22 ) , D425 ( 2 ) , epidermal ( 7 ) , HCT116 (intestinal) ( 20 ) , heart ( 19 ) , HEK293-A (epithelial) ( 22 ) , HeLa (cervical) ( 9 ) , intestine ( 9 ) , JB6 CI41 (epidermal) ( 23 ) , lung ( 18 ) , lymph node ( 8 ) , lymphocyte ( 6 ) , macrophage ( 1 ) , macrophage-peritoneum ( 14 ) , MEF (fibroblast) [IGF1R (mouse)] ( 22 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 15 ) , MIA PaCa-2 (pancreatic) ( 3 ) , myocyte-heart ( 19 ) , NCI-H128 (pulmonary) ( 4 ) , NCI-H520 (squamous) ( 4 ) , NPC (neural crest) ( 2 ) , OCI-ly3 (B lymphocyte) ( 5 ) , PANC-1 (pancreatic) ( 3 ) , RAW 264.7 (macrophage) ( 12 ) , REF52 (fibroblast) ( 22 ) , spleen ( 17 , 18 ) , SU-DHL-16 (B lymphocyte) ( 5 ) , T lymphocyte-spleen ( 10 , 16 )

Upstream Regulation
Regulatory protein:
ERK2 (rat) ( 19 ) , P38A (mouse) ( 23 ) , PKCE (mouse) ( 21 )
Putative upstream phosphatases:
DULLARD (human) ( 2 )
Phosphatases, in vitro:
PPP2CA (human) ( 22 )
Treatments:
EGF ( 23 ) , IL-2 ( 10 ) , IL-33 ( 12 ) , Matrine ( 5 ) , okadaic_acid ( 22 ) , rapamycin ( 1 ) , Sepantronium ( 3 ) , serum ( 22 ) , siRNA ( 2 ) , TGF-beta ( 1 ) , UV ( 21 )

Downstream Regulation
Effects of modification on Myc:
molecular association, regulation ( 22 ) , phosphorylation ( 22 ) , protein degradation ( 4 ) , protein stabilization ( 2 , 3 , 5 , 22 )
Effects of modification on biological processes:
apoptosis, inhibited ( 2 ) , carcinogenesis, induced ( 2 , 3 ) , carcinogenesis, inhibited ( 4 ) , cell cycle regulation ( 5 ) , cell growth, induced ( 2 , 3 , 5 ) , cell growth, inhibited ( 4 ) , signaling pathway regulation ( 5 ) , transcription, altered ( 22 ) , transcription, induced ( 1 )
Induce interaction with:
PIN1 (mouse) ( 22 )

Disease / Diagnostics Relevance
Relevant diseases:
B cell lymphoma ( 4 ) , T cell lymphoma ( 4 )

References 

1

Gauthier T, et al. (2023) TGF-β uncouples glycolysis and inflammation in macrophages and controls survival during sepsis. Sci Signal 16, eade0385
37552767   Curated Info

2

Luo Z, et al. (2023) Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability. Nat Commun 14, 762
36765089   Curated Info

3

Chang WH, et al. (2022) Oncogenic RAS promotes MYC protein stability by upregulating the expression of the inhibitor of apoptosis protein (IAP) family member Survivin. J Biol Chem, 102842
36581205   Curated Info

4

Daniel CJ, et al. (2022) T-cell dysfunction upon expression of MYC with altered phosphorylation at Threonine 58 and Serine 62. Mol Cancer Res
35380701   Curated Info

5

Gu J, et al. (2021) Matrine suppresses cell growth of diffuse large B-cell lymphoma via inhibiting CaMKIIγ/c-Myc/CDK6 signaling pathway. BMC Complement Med Ther 21, 163
34088288   Curated Info

6

Fish K, et al. (2020) Rewiring of B cell receptor signaling by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A
33020271   Curated Info

7

Wang X, et al. (2020) Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma. Oncogenesis
32895364   Curated Info

8

Guo H, et al. (2018) Dual inhibition of PI3K signaling and histone deacetylation halts proliferation and induces lethality in mantle cell lymphoma. Oncogene
30361685   Curated Info

9

Myant K, et al. (2015) Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation. Cell Rep 12, 1019-31
26235622   Curated Info

10

Preston GC, et al. (2015) Single cell tuning of Myc expression by antigen receptor signal strength and interleukin-2 in T lymphocytes. EMBO J 34, 2008-24
26136212   Curated Info

11

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

12

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

13

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

14

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

15

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

16

Navarro MN, et al. (2011) Phosphoproteomic analysis reveals an intrinsic pathway for the regulation of histone deacetylase 7 that controls the function of cytotoxic T lymphocytes. Nat Immunol 12, 352-61
21399638   Curated Info

17

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

18

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

19

Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83
19060905   Curated Info

20

Zhang Y, Wang Z, Li X, Magnuson NS (2008) Pim kinase-dependent inhibition of c-Myc degradation. Oncogene 27, 4809-19
18438430   Curated Info

21

Aziz MH, Manoharan HT, Verma AK (2007) Protein kinase C epsilon, which sensitizes skin to sun's UV radiation-induced cutaneous damage and development of squamous cell carcinomas, associates with Stat3. Cancer Res 67, 1385-94
17283176   Curated Info

22

Yeh E, et al. (2004) A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol 6, 308-18
15048125   Curated Info

23

He Z, et al. (2003) p38 Mitogen-activated protein kinase regulation of JB6 Cl41 cell transformation promoted by epidermal growth factor. J Biol Chem 278, 26435-42
12748197   Curated Info