Ser2448
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Home > Phosphorylation Site Page: > Ser2448  -  mTOR (rat)

Site Information
RSRTRTDsYSAGQSV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447578

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 ) , immunoprecipitation ( 15 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ) , western blotting ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 17 , 19 , 20 , 21 , 24 )
Disease tissue studied:
breast cancer ( 6 ) , liver cancer ( 6 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 4 ) , 'brain, hippocampus' ( 4 , 10 ) , 'brain, striatum' ( 10 ) , 'muscle, skeletal' ( 7 , 12 , 13 , 15 , 16 , 21 , 22 , 23 , 24 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 1 ) , granulosa ( 8 ) , heart ( 11 , 14 , 18 ) , hepatocyte-liver ( 19 ) , IEC-18 (epithelial) ( 17 ) , liver ( 6 ) , mammary gland ( 6 ) , myoblast ( 5 ) , RAEC (endothelial) ( 9 ) , RIN (epithelial) ( 20 ) , VSMC ( 3 )

Upstream Regulation
Regulatory protein:
EEA1 (human) ( 3 )
Treatments:
acadesine ( 23 ) , amino_acids ( 19 , 21 , 24 ) , angiotensin ( 17 ) , angiotensin_2 ( 3 ) , cAMP_analog ( 19 ) , CPCCOEt ( 10 ) , development ( 13 ) , DHPG ( 10 ) , ethanol ( 18 , 21 ) , exercise ( 22 ) , food deprivation ( 6 ) , glucagon ( 19 ) , glucosamine ( 20 ) , hypoxia ( 9 ) , IGF-1 ( 12 ) , indinavir ( 16 ) , insulin ( 5 , 16 , 19 , 20 , 24 ) , Intralipid ( 12 ) , ischemia ( 4 ) , leucine ( 7 , 12 ) , meal feeding ( 11 , 15 ) , mechanical stress ( 13 ) , MPEP ( 10 ) , muscle atrophy ( 24 ) , muscle contraction ( 24 ) , novel object recognition test ( 1 ) , piclidenoson ( 14 ) , rapamycin ( 9 , 17 ) , sepsis ( 7 ) , stress ( 1 ) , testosterone ( 5 ) , TGF-beta ( 8 ) , urofollitropin ( 8 )

Downstream Regulation
Effects of modification on biological processes:
translation, altered ( 24 )

References 

1

Calabrese F, et al. (2017) Chronic Mild Stress-Induced Alterations of Local Protein Synthesis: A Role for Cognitive Impairment. ACS Chem Neurosci 8, 817-825
28094500   Curated Info

2

Srivastava T, et al. (2012) Regulation of neuronal mRNA translation by CaM-kinase I phosphorylation of eIF4GII. J Neurosci 32, 5620-30
22514323   Curated Info

3

Nazarewicz RR, et al. (2011) Early endosomal antigen 1 (EEA1) is an obligate scaffold for angiotensin II-induced, PKC-alpha-dependent Akt activation in endosomes. J Biol Chem 286, 2886-95
21097843   Curated Info

4

Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem 285, 34355-63
20736160   Curated Info

5

Allemand MC, et al. (2009) Effect of testosterone on insulin stimulated IRS1 Ser phosphorylation in primary rat myotubes--a potential model for PCOS-related insulin resistance. PLoS One 4, e4274
19169352   Curated Info

6

Jiang W, Zhu Z, Thompson HJ (2008) Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver. Cancer Res 68, 5492-9
18593953   Curated Info

7

Vary TC (2007) Acute oral leucine administration stimulates protein synthesis during chronic sepsis through enhanced association of eukaryotic initiation factor 4G with eukaryotic initiation factor 4E in rats. J Nutr 137, 2074-9
17709445   Curated Info

8

Chen YJ, et al. (2007) Interplay of PI3K and cAMP/PKA signaling, and rapamycin-hypersensitivity in TGFbeta1 enhancement of FSH-stimulated steroidogenesis in rat ovarian granulosa cells. J Endocrinol 192, 405-19
17283241   Curated Info

9

Li W, et al. (2007) Hypoxia-induced endothelial proliferation requires both mTORC1 and mTORC2. Circ Res 100, 79-87
17110594   Curated Info

10

Page G, et al. (2006) Group I metabotropic glutamate receptors activate the p70S6 kinase via both mammalian target of rapamycin (mTOR) and extracellular signal-regulated kinase (ERK 1/2) signaling pathways in rat striatal and hippocampal synaptoneurosomes. Neurochem Int 49, 413-21
16549223   Curated Info

11

Vary TC, Lynch CJ (2006) Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts. J Nutr 136, 2284-90
16920842   Curated Info

12

Lang CH (2006) Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle. Am J Physiol Endocrinol Metab 291, E666-74
16684854   Curated Info

13

Thomson DM, Gordon SE (2006) Impaired overload-induced muscle growth is associated with diminished translational signalling in aged rat fast-twitch skeletal muscle. J Physiol 574, 291-305
16627569   Curated Info

14

Park SS, et al. (2006) N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide confers cardioprotection at reperfusion by inhibiting mitochondrial permeability transition pore opening via glycogen synthase kinase 3 beta. J Pharmacol Exp Ther 318, 124-31
16611852   Curated Info

15

Vary TC, Lynch CJ (2006) Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation. Am J Physiol Endocrinol Metab 290, E631-42
16263769   Curated Info

16

Hong-Brown LQ, et al. (2005) Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle. Am J Physiol Endocrinol Metab 289, E382-90
15827064   Curated Info

17

Chiu T, Santiskulvong C, Rozengurt E (2005) EGF receptor transactivation mediates ANG II-stimulated mitogenesis in intestinal epithelial cells through the PI3-kinase/Akt/mTOR/p70S6K1 signaling pathway. Am J Physiol Gastrointest Liver Physiol 288, G182-94
15358595   Curated Info

18

Vary TC, Deiter G, Goodman SA (2005) Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling. Am J Physiol Heart Circ Physiol 288, H121-8
15388509   Curated Info

19

Mothe-Satney I, et al. (2004) In rat hepatocytes glucagon increases mammalian target of rapamycin phosphorylation on serine 2448 but antagonizes the phosphorylation of its downstream targets induced by insulin and amino acids. J Biol Chem 279, 42628-37
15292249   Curated Info

20

Andreozzi F, et al. (2004) Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells. Endocrinology 145, 2845-57
15001544   Curated Info

21

Lang CH, et al. (2003) Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle. Am J Physiol Endocrinol Metab 285, E1205-15
12944322   Curated Info

22

Bolster DR, et al. (2003) Immediate response of mammalian target of rapamycin (mTOR)-mediated signalling following acute resistance exercise in rat skeletal muscle. J Physiol 553, 213-20
12937293   Curated Info

23

Bolster DR, Crozier SJ, Kimball SR, Jefferson LS (2002) AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling. J Biol Chem 277, 23977-80
11997383   Curated Info

24

Reynolds TH, Bodine SC, Lawrence JC (2002) Control of Ser2448 phosphorylation in the mammalian target of rapamycin by insulin and skeletal muscle load. J Biol Chem 277, 17657-62
11884412   Curated Info