Ser516
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Home > Phosphorylation Site Page: > Ser516  -  SCN5A (human)

Site Information
LsLTrGLsRTsMKPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4719558

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 4 ) , mass spectrometry (in vitro) ( 1 , 2 ) , mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 3 ) , heart ( 1 ) , HEK293T (epithelial) ( 2 ) , HeLa (cervical) ( 4 )

Upstream Regulation
Putative in vivo kinases:
CAMK2A (human) ( 1 )
Kinases, in vitro:
CAMK2A (human) ( 1 ) , CAMK2D (human) ( 3 ) , CAMK2D (rat) ( 3 ) , PKACA (human) ( 2 )
Treatments:
BI2536 ( 4 )

Downstream Regulation
Effects of modification on SCN5A:
activity, inhibited ( 3 ) , methylation ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
cardiomyopathy ( 1 ) , DCM ( 1 )

References 

1

Herren AW, et al. (2015) CaMKII Phosphorylation of Na(V)1.5: Novel in Vitro Sites Identified by Mass Spectrometry and Reduced S516 Phosphorylation in Human Heart Failure. J Proteome Res 14, 2298-311
25815641   Curated Info

2

Beltran-Alvarez P, et al. (2015) Interplay between R513 methylation and S516 phosphorylation of the cardiac voltage-gated sodium channel. Amino Acids 47, 429-34
25501501   Curated Info

3

Ashpole NM, et al. (2012) Ca2+/Calmodulin-dependent Protein Kinase II (CaMKII) Regulates Cardiac Sodium Channel NaV1.5 Gating by Multiple Phosphorylation Sites. J Biol Chem 287, 19856-69
22514276   Curated Info

4

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

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