Ser163
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Home > Phosphorylation Site Page: > Ser163  -  MDM2 (mouse)

Site Information
SSRRRsIsEtEENTD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448130

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 1 , 4 , 5 , 6 ) , mutation of modification site ( 7 ) , phospho-antibody ( 2 , 3 , 8 , 9 , 10 ) , western blotting ( 2 , 3 , 8 , 9 , 10 )
Disease tissue studied:
melanoma skin cancer ( 9 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 , 5 ) , epithelial-mammary gland [MDM2 (mouse), transgenic] ( 7 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , macrophage-bone marrow ( 2 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 6 ) , mammary gland [MDM2 (mouse), transgenic] ( 7 ) , MEF (fibroblast) ( 8 ) , melanocyte-skin [IKKB (mouse), homozygous knockout] ( 9 ) , parotid acinar-salivary gland ( 10 ) , parotid acinar-salivary gland [Akt1 (mouse)] ( 10 )

Upstream Regulation
Regulatory protein:
Akt1 (mouse) ( 10 ) , CK1A (mouse) ( 3 ) , IKKB (mouse) ( 9 ) , P38A (mouse) ( 8 ) , p70S6K (mouse) ( 8 ) , P70S6KB (mouse) ( 8 ) , TSC1 (mouse) ( 8 ) , TSC2 (mouse) ( 8 )
Putative in vivo kinases:
Akt1 (mouse) ( 2 ) , p70S6K (mouse) ( 8 ) , PIK3CA (mouse) ( 2 )
Kinases, in vitro:
p70S6K (human) ( 8 )
Treatments:
adriamycin ( 8 ) , amino_acid_starvation ( 8 ) , insulin ( 5 ) , LY294002 ( 2 , 5 ) , MK-2206 ( 5 ) , nutlin-3 ( 2 ) , rapamycin ( 8 ) , SB203580 ( 8 ) , Torin1 ( 8 )

Downstream Regulation
Effects of modification on MDM2:
intracellular localization ( 8 )
Effects of modification on biological processes:
apoptosis, inhibited ( 7 ) , carcinogenesis, induced ( 7 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 7 )

References 

1

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

2

Li L, et al. (2015) A unique role for p53 in the regulation of M2 macrophage polarization. Cell Death Differ 22, 1081-93
25526089   Curated Info

3

Bowman BM, et al. (2015) Phosphorylation of FADD by the kinase CK1α promotes KRASG12D-induced lung cancer. Sci Signal 8, ra9
25628462   Curated Info

4

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

7

Cheng X, et al. (2010) Activation of murine double minute 2 by Akt in mammary epithelium delays mammary involution and accelerates mammary tumorigenesis. Cancer Res 70, 7684-9
20841468   Curated Info

8

Lai KP, et al. (2010) S6K1 is a multifaceted regulator of Mdm2 that connects nutrient status and DNA damage response. EMBO J 29, 2994-3006
20657550   Curated Info

9

Yang J, et al. (2010) Conditional ablation of Ikkb inhibits melanoma tumor development in mice. J Clin Invest 120, 2563-74
20530876   Curated Info

10

Limesand KH, Schwertfeger KL, Anderson SM (2006) MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells. Mol Cell Biol 26, 8840-56
16982679   Curated Info