Ser156
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Home > Phosphorylation Site Page: > Ser156  -  MARCKS (mouse)

Site Information
KRFsFKKsFkLsGFs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447787

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 , 6 , 7 , 11 , 12 , 13 , 14 , 16 , 18 , 19 , 20 , 21 ) , mutation of modification site ( 15 ) , phospho-antibody ( 8 , 9 , 10 , 17 , 22 , 23 , 24 ) , western blotting ( 8 , 9 , 10 , 17 , 23 , 24 )
Disease tissue studied:
anthrax infection ( 13 ) , melanoma skin cancer ( 15 , 18 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 , 6 ) , 'brain, cerebral cortex' [PCDHGA1 (mouse), homozygous knockout] ( 8 ) , 'brain, embryonic' ( 21 ) , 'fat, brown' ( 14 ) , 308 (keratinocyte) ( 10 ) , B16F1 (melanocyte) ( 15 ) , B16F10 ( 15 ) , bone [PKCD (mouse)] ( 22 ) , brain ( 14 , 16 , 19 , 20 ) , COS (fibroblast) ( 23 ) , heart ( 7 , 14 ) , kidney ( 14 ) , liver ( 14 ) , lung ( 14 ) , MC3T3-E1 (preosteoblast) ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 11 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 12 ) , osteoblast [PKCD (mouse)] ( 22 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 18 ) , spleen ( 13 , 14 ) , Swiss 3T3 (fibroblast) ( 9 , 17 , 23 , 24 ) , testis ( 14 )

Upstream Regulation
Regulatory protein:
PCDHGA1 (mouse) ( 8 )
Putative in vivo kinases:
PKACA (mouse) ( 15 )
Kinases, in vitro:
PKN1 (human) ( 25 )
Treatments:
bombesin ( 17 , 24 ) , BSO ( 23 ) , calphostin_C ( 15 ) , GF109203X ( 17 , 23 ) , Go_6976 ( 10 ) , H2O2 ( 23 ) , insulin ( 6 ) , NAC ( 23 ) , okadaic_acid ( 15 ) , phorbol_ester ( 10 , 23 ) , PP2 ( 23 ) , PTH(1-34) ( 4 ) , vasopressin ( 24 ) , Wnt3a ( 22 )

Downstream Regulation
Effects of modification on MARCKS:
intracellular localization ( 15 )
Effects of modification on biological processes:
carcinogenesis, induced ( 15 ) , cell motility, altered ( 15 )

Disease / Diagnostics Relevance
Relevant diseases:
melanoma skin cancer ( 15 )

References 

1

Semenza ER, et al. (2021) D-cysteine is an endogenous regulator of neural progenitor cell dynamics in the mammalian brain. Proc Natl Acad Sci U S A 118
34556581   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

4

Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50
26160508   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

7

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

8

Garrett AM, Schreiner D, Lobas MA, Weiner JA (2012) γ-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74, 269-76
22542181   Curated Info

9

Waldron RT, et al. (2012) Differential PKC-dependent and -independent PKD activation by G protein α subunits of the Gq family: selective stimulation of PKD Ser⁷⁴⁸ autophosphorylation by Gαq. Cell Signal 24, 914-21
22227248   Curated Info

10

Pan X, Kane LA, Van Eyk JE, Coulombe PA (2011) Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion. J Biol Chem 286, 42403-13
22006917   Curated Info

11

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

12

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

13

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

14

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

15

Chen X, Rotenberg SA (2010) PhosphoMARCKS drives motility of mouse melanoma cells. Cell Signal 22, 1097-103
20211725   Curated Info

16

Zhou J (2009) CST Curation Set: 7414; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

17

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

18

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

19

Zhou J (2008) CST Curation Set: 4863; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

20

Possemato A (2008) CST Curation Set: 3854; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

21

Possemato A (2008) CST Curation Set: 3829; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

22

Tu X, et al. (2007) Noncanonical Wnt signaling through G protein-linked PKCdelta activation promotes bone formation. Dev Cell 12, 113-27
17199045   Curated Info

23

Waldron RT, Rey O, Zhukova E, Rozengurt E (2004) Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D. J Biol Chem 279, 27482-93
15084589   Curated Info

24

Sinnett-Smith J, et al. (2004) Protein kinase D potentiates DNA synthesis induced by Gq-coupled receptors by increasing the duration of ERK signaling in swiss 3T3 cells. J Biol Chem 279, 16883-93
14963034   Curated Info

25

Palmer RH, et al. (1996) PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163. FEBS Lett 378, 281-5
8557118   Curated Info