Ser152
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Home > Phosphorylation Site Page: > Ser152  -  MARCKS (mouse)

Site Information
kkKKKRFsFKKsFkL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447786

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 , 5 , 11 , 12 ) , mutation of modification site ( 9 ) , phospho-antibody ( 6 , 7 , 8 , 10 , 13 , 14 , 15 , 16 ) , western blotting ( 6 , 7 , 8 , 10 , 15 , 16 )
Disease tissue studied:
melanoma skin cancer ( 9 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'brain, cerebral cortex' [PCDHGA1 (mouse), homozygous knockout] ( 6 ) , 'brain, embryonic' ( 12 ) , 308 (keratinocyte) ( 8 ) , B16F1 (melanocyte) ( 9 ) , B16F10 ( 9 ) , bone [PKCD (mouse)] ( 13 ) , brain ( 11 ) , COS (fibroblast) ( 15 ) , liver ( 5 ) , liver [leptin (mouse), homozygous knockout] ( 5 ) , macrophage-peritoneum ( 4 ) , MC3T3-E1 (preosteoblast) ( 3 ) , osteoblast [PKCD (mouse)] ( 13 ) , spinal cord ( 14 ) , Swiss 3T3 (fibroblast) ( 7 , 10 , 15 , 16 )

Upstream Regulation
Regulatory protein:
leptin (mouse) ( 5 ) , PCDHGA1 (mouse) ( 6 )
Putative in vivo kinases:
PKACA (mouse) ( 9 ) , ROCK1 (mouse) ( 14 )
Kinases, in vitro:
PKN1 (human) ( 17 ) , ROCK1 (mouse) ( 14 )
Treatments:
bombesin ( 10 , 16 ) , BSO ( 15 ) , calphostin_C ( 9 ) , GF109203X ( 10 , 15 ) , Go_6976 ( 8 ) , H1152 ( 14 ) , H2O2 ( 15 ) , NAC ( 15 ) , nerve_damage ( 14 ) , okadaic_acid ( 9 ) , phorbol_ester ( 8 , 15 ) , PP2 ( 15 ) , PTH(1-34) ( 3 ) , vasopressin ( 16 ) , Wnt3a ( 13 )

Downstream Regulation
Effects of modification on MARCKS:
intracellular localization ( 9 )
Effects of modification on biological processes:
carcinogenesis, induced ( 9 ) , cell motility, altered ( 9 )

Disease / Diagnostics Relevance
Relevant diseases:
melanoma skin cancer ( 9 )

References 

1

Semenza ER, et al. (2021) D-cysteine is an endogenous regulator of neural progenitor cell dynamics in the mammalian brain. Proc Natl Acad Sci U S A 118
34556581   Curated Info

2

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

3

Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50
26160508   Curated Info

4

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

5

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

6

Garrett AM, Schreiner D, Lobas MA, Weiner JA (2012) γ-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74, 269-76
22542181   Curated Info

7

Waldron RT, et al. (2012) Differential PKC-dependent and -independent PKD activation by G protein α subunits of the Gq family: selective stimulation of PKD Ser⁷⁴⁸ autophosphorylation by Gαq. Cell Signal 24, 914-21
22227248   Curated Info

8

Pan X, Kane LA, Van Eyk JE, Coulombe PA (2011) Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion. J Biol Chem 286, 42403-13
22006917   Curated Info

9

Chen X, Rotenberg SA (2010) PhosphoMARCKS drives motility of mouse melanoma cells. Cell Signal 22, 1097-103
20211725   Curated Info

10

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

11

Zhou J (2008) CST Curation Set: 4863; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

12

Possemato A (2008) CST Curation Set: 3829; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

13

Tu X, et al. (2007) Noncanonical Wnt signaling through G protein-linked PKCdelta activation promotes bone formation. Dev Cell 12, 113-27
17199045   Curated Info

14

Tatsumi S, et al. (2005) Involvement of Rho-kinase in inflammatory and neuropathic pain through phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS). Neuroscience 131, 491-8
15708490   Curated Info

15

Waldron RT, Rey O, Zhukova E, Rozengurt E (2004) Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D. J Biol Chem 279, 27482-93
15084589   Curated Info

16

Sinnett-Smith J, et al. (2004) Protein kinase D potentiates DNA synthesis induced by Gq-coupled receptors by increasing the duration of ERK signaling in swiss 3T3 cells. J Biol Chem 279, 16883-93
14963034   Curated Info

17

Palmer RH, et al. (1996) PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163. FEBS Lett 378, 281-5
8557118   Curated Info