Thr208
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Home > Phosphorylation Site Page: > Thr208  -  MAPKAPK2 (mouse)

Site Information
TSHNsLttPCYtPYY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448269

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 1 , 2 , 6 ) , mutation of modification site ( 8 ) , phospho-antibody ( 3 , 4 , 5 , 7 ) , western blotting ( 3 , 4 , 5 , 7 )
Disease tissue studied:
lymphoma ( 4 ) , T cell lymphoma ( 4 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 5 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 8 ) , brain ( 1 , 2 ) , embryo ( 1 ) , heart ( 7 ) , HeLa (cervical) ( 3 ) , liver ( 1 , 6 ) , MEF (fibroblast) ( 3 ) , S49 (T lymphocyte) ( 4 )

Upstream Regulation
Kinases, in vitro:
ERK1 (mouse) ( 8 )
Treatments:
cAMP_analog ( 4 ) , heat_shock ( 8 ) , ischemia/reperfusion ( 7 ) , nerve_damage ( 5 ) , norepinephrine ( 4 ) , SB202190 ( 7 )

Downstream Regulation
Effects of modification on MAPKAPK2:
enzymatic activity, induced ( 8 )

References 

1

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

2

Guo A (2011) CST Curation Set: 12477; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

3

Ronkina N, et al. (2011) Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes. Nucleic Acids Res 39, 2503-18
21109534   Curated Info

4

Lajevic MD, Suleiman S, Cohen RL, Chambers DA (2011) Activation of p38 mitogen-activated protein kinase by norepinephrine in T-lineage cells. Immunology 132, 197-208
21039464   Curated Info

5

Norrby M, T├ągerud S (2010) Mitogen-activated protein kinase-activated protein kinase 2 (MK2) in skeletal muscle atrophy and hypertrophy. J Cell Physiol 223, 194-201
20049871   Curated Info

6

Zhou J (2008) CST Curation Set: 4866; Year: 2008; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

7

Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44
17993600   Curated Info

8

Engel K, et al. (1995) Constitutive activation of mitogen-activated protein kinase-activated protein kinase 2 by mutation of phosphorylation sites and an A-helix motif. J Biol Chem 270, 27213-21
7592979   Curated Info