Ser431
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Home > Phosphorylation Site Page: > Ser431  -  LKB1 (mouse)

Site Information
sNKIRRLsACKQQ__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448147

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 15 ) , immunoassay ( 1 ) , mass spectrometry ( 5 , 16 ) , microscopy-colocalization with upstream kinase ( 11 ) , mutation of modification site ( 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ) , peptide sequencing ( 15 , 16 ) , phospho-antibody ( 1 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 16 ) , phosphoamino acid analysis ( 15 ) , phosphopeptide mapping ( 15 , 16 ) , western blotting ( 1 , 4 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 16 , 17 )
Disease tissue studied:
cervical cancer ( 10 , 13 ) , cervical adenocarcinoma ( 10 , 13 ) , lung cancer ( 1 , 10 , 13 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 11 ) , 'neuron, hippocampal'-'brain, embryonic' ( 12 ) , 293 (epithelial) ( 8 , 15 , 16 ) , 3T3-L1 (fibroblast) ( 13 ) , A549 (pulmonary) ( 1 , 10 , 13 ) , aorta ( 10 ) , BAEC (endothelial) ( 10 , 13 ) , brain ( 5 ) , Chang liver (cervical) ( 9 ) , CV1 (fibroblast) ( 17 ) , embryo ( 5 ) , ES (stem) ( 16 ) , G361 (melanocyte) ( 14 ) , HEK293T (epithelial) ( 12 ) , HeLa (cervical) ( 11 ) , HeLa S3 (cervical) ( 10 , 13 ) , hepatocyte ( 7 ) , HUVEC (endothelial) ( 10 ) , liver ( 5 ) , lung ( 1 ) , NCI-H1299 (pulmonary) ( 1 ) , placenta ( 6 ) , Rat2 (fibroblast) ( 16 ) , retina ( 13 ) , vascular smooth muscle cell ('muscle, smooth') ( 13 )

Upstream Regulation
Regulatory protein:
Cas-L (human) ( 1 ) , PKCZ (human) ( 13 ) , PKCZ (mouse) ( 10 ) , PKIB (mouse) ( 4 ) , SIRT6 (human) ( 7 )
Putative in vivo kinases:
ATM (mouse) ( 14 ) , p90RSK (mouse) ( 16 ) , PKACA (mouse) ( 16 , 17 ) , PKCZ (human) ( 13 )
Kinases, in vitro:
ATM (mouse) ( 14 ) , DNAPK (mouse) ( 14 ) , MSK1 (mouse) ( 16 ) , p70S6K (mouse) ( 16 ) , p90RSK (mouse) ( 16 ) , PKACA (mouse) ( 16 )
Treatments:
adiponectin ( 6 ) , BDNF ( 12 ) , cAMP_analog ( 16 ) , colforsin ( 4 , 8 , 12 , 14 , 16 ) , dasatinib ( 1 ) , EGF ( 14 , 16 ) , free_fatty_acids ( 7 ) , H-89 ( 16 ) , high_glucose ( 4 ) , KT5720 ( 12 ) , low_glucose ( 4 ) , ONOO(-) ( 13 ) , PD184352 ( 16 ) , PD98059 ( 16 ) , PF-573228 ( 1 ) , phorbol_ester ( 14 ) , rapamycin ( 16 ) , Ro31-8220 ( 16 ) , rolipram ( 12 ) , rosiglitazone ( 7 ) , simvastatin ( 10 ) , siRNA ( 10 ) , STO-609 ( 1 ) , U0126 ( 16 ) , wortmannin ( 16 )

Downstream Regulation
Effects of modification on LKB1:
enzymatic activity, induced ( 11 ) , phosphorylation ( 11 )
Effects of modification on biological processes:
autophagy, induced ( 1 ) , carcinogenesis, inhibited ( 1 ) , cell differentiation, induced ( 12 ) , cell growth, altered ( 16 ) , cytoskeletal reorganization ( 11 )
Induce interaction with:
AMPKA1 (mouse) ( 11 ) , BRSK1 (mouse) ( 11 ) , Tau (mouse) ( 11 )

References 

1

Deneka AY, et al. (2021) Nedd9 restrains autophagy to limit growth of early stage non-small cell lung cancer. Cancer Res
34006524   Curated Info

2

Liu Y, et al. (2020) TLR9 and beclin┬┐┬┐1 crosstalk regulates muscle AMPK activation in exercise. Nature
32051584   Curated Info

3

Zuo A, et al. (2020) CTRP9 knockout exaggerates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy through inhibiting the LKB1/AMPK pathway. J Cell Mol Med
31930700   Curated Info

4

Blanchet E, et al. (2015) Feedback inhibition of CREB signaling promotes beta cell dysfunction in insulin resistance. Cell Rep 10, 1149-57
25704817   Curated Info

5

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

6

Rosario FJ, et al. (2012) Chronic maternal infusion of full-length adiponectin in pregnant mice down-regulates placental amino acid transporter activity and expression and decreases fetal growth. J Physiol 590, 1495-509
22289908   Curated Info

7

Yang SJ, et al. (2011) Activation of peroxisome proliferator-activated receptor gamma by rosiglitazone increases sirt6 expression and ameliorates hepatic steatosis in rats. PLoS One 6, e17057
21373642   Curated Info

8

Fogarty S, Hardie DG (2009) C-terminal phosphorylation of LKB1 is not required for regulation of AMP-activated protein kinase, BRSK1, BRSK2, or cell cycle arrest. J Biol Chem 284, 77-84
18854318   Curated Info

9

Denison FC, Hiscock NJ, Carling D, Woods A (2009) Characterization of an alternative splice variant of LKB1. J Biol Chem 284, 67-76
18854309   Curated Info

10

Choi HC, et al. (2008) Reactive nitrogen species is required for the activation of the AMP-activated protein kinase by statin in vivo. J Biol Chem 283, 20186-97
18474592   Curated Info

11

Barnes AP, et al. (2007) LKB1 and SAD kinases define a pathway required for the polarization of cortical neurons. Cell 129, 549-63
17482548   Curated Info

12

Shelly M, et al. (2007) LKB1/STRAD promotes axon initiation during neuronal polarization. Cell 129, 565-77
17482549   Curated Info

13

Xie Z, et al. (2006) Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells. J Biol Chem 281, 6366-75
16407220   Curated Info

14

Sapkota GP, et al. (2002) Ionizing radiation induces ataxia telangiectasia mutated kinase (ATM)-mediated phosphorylation of LKB1/STK11 at Thr-366. Biochem J 368, 507-16
12234250   Curated Info

15

Sapkota GP, et al. (2002) Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem J 362, 481-90
11853558   Curated Info

16

Sapkota GP, et al. (2001) Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth. J Biol Chem 276, 19469-82
11297520   Curated Info

17

Collins SP, Reoma JL, Gamm DM, Uhler MD (2000) LKB1, a novel serine/threonine protein kinase and potential tumour suppressor, is phosphorylated by cAMP-dependent protein kinase (PKA) and prenylated in vivo. Biochem J 345 Pt 3, 673-80
10642527   Curated Info