Thr207
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr207  -  NKCC1 (human)

Site Information
YYDtHTNtyYLRtFG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 467236

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 3 ) , mass spectrometry ( 2 ) , mutation of modification site ( 3 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 4 ) , 293 (epithelial) ( 2 , 3 , 5 , 7 ) , brain ( 1 ) , erythrocyte-blood ( 6 ) , fibroblast-skin ( 3 )

Upstream Regulation
Regulatory protein:
CAB39 (human) ( 5 ) , OSR1 (human) ( 4 ) , WNK1 (human) ( 2 ) , WNK3 (human) ( 2 )
Putative in vivo kinases:
OSR1 (human) ( 5 , 7 )
Kinases, in vitro:
OSR1 (human) ( 5 , 7 ) , STLK3 (human) ( 7 )
Treatments:
A-769662 ( 6 ) , hypotonic_buffer ( 5 ) , intraventricular hemorrhage ( 1 ) , low_chloride ( 3 , 4 ) , siRNA ( 5 ) , sorbitol ( 4 , 7 ) , sucrose ( 6 )

Downstream Regulation
Effects of modification on NKCC1:
activity, induced ( 6 , 7 ) , enzymatic activity, induced ( 4 )

References 

1

Karimy JK, et al. (2017) Inflammation-dependent cerebrospinal fluid hypersecretion by the choroid plexus epithelium in posthemorrhagic hydrocephalus. Nat Med 23, 997-1003
28692063   Curated Info

2

Zhang J, et al. (2016) Functional kinomics establishes a critical node of volume-sensitive cation-Cl- cotransporter regulation in the mammalian brain. Sci Rep 6, 35986
27782176   Curated Info

3

Kahle KT, et al. (2016) Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter. Sci Signal 9, ra77
27485015   Curated Info

4

Thastrup JO, et al. (2012) SPAK/OSR1 regulate NKCC1 and WNK activity: analysis of WNK isoform interactions and activation by T-loop trans-autophosphorylation. Biochem J 441, 325-37
22032326   Curated Info

5

Filippi BM, et al. (2011) MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases. EMBO J 30, 1730-41
21423148   Curated Info

6

Sid B, et al. (2010) Stimulation of human and mouse erythrocyte Na(+)-K(+)-2Cl(-) cotransport by osmotic shrinkage does not involve AMP-activated protein kinase, but is associated with STE20/SPS1-related proline/alanine-rich kinase activation. J Physiol 588, 2315-28
20442269   Curated Info

7

Vitari AC, et al. (2006) Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1. Biochem J 397, 223-31
16669787   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.