Ser298
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Home > Phosphorylation Site Page: > Ser298  -  MEK1 (rat)

Site Information
RtPGRPLsSYGMDSR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448284

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
COS (fibroblast) ( 3 ) , REF52 (fibroblast) ( 1 , 3 ) , RIE (epithelial) ( 2 ) , RIE (epithelial) [PPAR-gamma (mouse)] ( 2 )

Upstream Regulation
Regulatory protein:
PAK1 (rat) ( 2 , 3 )
Putative in vivo kinases:
PAK1 (rat) ( 1 , 3 )
Kinases, in vitro:
PAK2 (rat) ( 1 ) , PAK3 (human) ( 3 ) , PAK3 (rat) ( 1 )
Treatments:
EGF ( 1 ) , fibronectin ( 1 , 3 ) , LY294002 ( 2 ) , PP2 ( 3 ) , RS5444 ( 2 )

Downstream Regulation
Effects of modification on MEK1:
enzymatic activity, induced ( 1 , 3 ) , intracellular localization ( 3 ) , phosphorylation ( 1 )
Effects of modification on biological processes:
cell adhesion, altered ( 3 ) , cell cycle regulation ( 1 ) , cytoskeletal reorganization ( 3 )

References 

1

Park ER, Eblen ST, Catling AD (2007) MEK1 activation by PAK: a novel mechanism. Cell Signal 19, 1488-96
17314031   Curated Info

2

Chen L, et al. (2006) Peroxisome proliferator-activated receptor gamma promotes epithelial to mesenchymal transformation by Rho GTPase-dependent activation of ERK1/2. J Biol Chem 281, 24575-87
16815847   Curated Info

3

Slack-Davis JK, et al. (2003) PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation. J Cell Biol 162, 281-91
12876277   Curated Info