Ser32
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser32  -  IkB-alpha (rat)

Site Information
LVDDRHDsGLDsMKD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448203

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 4 , 5 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:
adrenal cancer ( 5 ) , pheochromocytoma ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , intestine ( 6 ) , mesangial ( 7 ) , NR8383 (macrophage) ( 8 ) , PC-12 (chromaffin) ( 5 , 9 ) , vascular smooth muscle cell ('muscle, smooth') ( 3 )

Upstream Regulation
Kinases, in vitro:
IKKB (rat) ( 2 ) , p90RSK (rat) ( 2 )
Treatments:
angiotensin ( 3 ) , Bay_11-7085 ( 6 ) , cell_detachment ( 4 ) , colforsin ( 7 ) , IL-1b ( 7 ) , ischemia/reperfusion ( 6 ) , LY294002 ( 5 ) , NGF ( 5 , 9 ) , PD98059 ( 5 ) , TNF ( 3 , 9 )

Downstream Regulation
Effects of modification on IkB-alpha:
activity, induced ( 5 ) , intracellular localization ( 8 )
Effects of modification on biological processes:
apoptosis, altered ( 4 ) , transcription, altered ( 5 )

References 

1

Tang CH, et al. (2007) Leptin-induced IL-6 production is mediated by leptin receptor, insulin receptor substrate-1, phosphatidylinositol 3-kinase, Akt, NF-kappaB, and p300 pathway in microglia. J Immunol 179, 1292-302
17617622   Curated Info

2

Xu S, Bayat H, Hou X, Jiang B (2006) Ribosomal S6 kinase-1 modulates interleukin-1beta-induced persistent activation of NF-kappaB through phosphorylation of IkappaBbeta. Am J Physiol Cell Physiol 291, C1336-45
16822942   Curated Info

3

Douillette A, et al. (2006) The proinflammatory actions of angiotensin II are dependent on p65 phosphorylation by the IkappaB kinase complex. J Biol Chem 281, 13275-84
16513650   Curated Info

4

Toruner M, et al. (2006) Antianoikis effect of nuclear factor-kappaB through up-regulated expression of osteoprotegerin, BCL-2, and IAP-1. J Biol Chem 281, 8686-96
16407217   Curated Info

5

Chen YL, Law PY, Loh HH (2006) Sustained activation of phosphatidylinositol 3-kinase/Akt/nuclear factor kappaB signaling mediates G protein-coupled delta-opioid receptor gene expression. J Biol Chem 281, 3067-74
16316997   Curated Info

6

Zou L, Attuwaybi B, Kone BC (2003) Effects of NF-kappa B inhibition on mesenteric ischemia-reperfusion injury. Am J Physiol Gastrointest Liver Physiol 284, G713-21
12466147   Curated Info

7

Takahashi N, Tetsuka T, Uranishi H, Okamoto T (2002) Inhibition of the NF-kappaB transcriptional activity by protein kinase A. Eur J Biochem 269, 4559-65
12230568   Curated Info

8

Barua M, Liu Y, Quinn MR (2001) Taurine chloramine inhibits inducible nitric oxide synthase and TNF-alpha gene expression in activated alveolar macrophages: decreased NF-kappaB activation and IkappaB kinase activity. J Immunol 167, 2275-81
11490015   Curated Info

9

Bui NT, Livolsi A, Peyron JF, Prehn JH (2001) Activation of nuclear factor kappaB and Bcl-x survival gene expression by nerve growth factor requires tyrosine phosphorylation of IkappaBalpha. J Cell Biol 152, 753-64
11266466   Curated Info