Tyr1253
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Home > Phosphorylation Site Page: > Tyr1253  -  PLCG1 (rat)

Site Information
EGsFEARyQQPFEDF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448576

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , mutation of modification site ( 2 , 4 ) , phospho-antibody ( 1 , 2 , 3 ) , phosphopeptide mapping ( 3 , 4 ) , western blotting ( 1 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
TrkB (rat) ( 3 )
Kinases, in vitro:
EGFR (human) ( 2 ) , Src (human) ( 2 ) , ZAP70 (human) ( 2 )
Treatments:
EGF ( 1 ) , neurotrophin-3 ( 3 ) , PDGF ( 2 , 4 )

Downstream Regulation
Effects of modification on PLCG1:
enzymatic activity, induced ( 4 )

References 

1

Chung SH, et al. (2010) A double point mutation in PCL-gamma1 (Y509A/F510A) enhances Y783 phosphorylation and inositol phospholipid-hydrolyzing activity upon EGF stimulation. Exp Mol Med 42, 216-27
20164676   Curated Info

2

Sekiya F, Poulin B, Kim YJ, Rhee SG (2004) Mechanism of tyrosine phosphorylation and activation of phospholipase C-gamma 1. Tyrosine 783 phosphorylation is not sufficient for lipase activation. J Biol Chem 279, 32181-90
15161916   Curated Info

3

Middlemas DS, Meisenhelder J, Hunter T (1994) Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor. J Biol Chem 269, 5458-66
8106527   Curated Info

4

Kim HK, et al. (1991) PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell 65, 435-41
1708307   Curated Info