Tyr783
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Home > Phosphorylation Site Page: > Tyr783  -  PLCG1 (rat)

Site Information
EGRNPGFyVEANPMP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447667
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 16 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 3 , 4 , 6 , 7 , 8 , 9 , 11 , 12 ) , mutation of modification site ( 1 , 10 , 13 , 16 ) , phospho-antibody ( 1 , 2 , 5 , 10 , 13 , 14 , 15 ) , phosphopeptide mapping ( 15 , 16 ) , western blotting ( 1 , 2 , 16 )
Disease tissue studied:
breast cancer ( 10 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 2 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 13 ) , 3T3 (fibroblast) ( 16 ) , A431 (epithelial) ( 13 ) , brain ( 3 , 4 , 6 , 7 , 8 , 9 , 11 , 12 ) , COS (fibroblast) ( 1 ) , HEK293T (epithelial) ( 5 ) , hepatocyte-liver ( 14 ) , Jurkat (T lymphocyte) ( 13 ) , kidney ( 5 ) , MTLn3 (breast cell) ( 10 ) , Null-TV1 [PLCG1 (rat)] ( 13 ) , RAMOS (B lymphocyte) ( 13 ) , Rat2 (fibroblast) ( 15 )

Upstream Regulation
Putative in vivo kinases:
TrkB (rat) ( 15 )
Kinases, in vitro:
EGFR (human) ( 13 ) , Src (human) ( 13 ) , ZAP70 (human) ( 13 )
Treatments:
BDNF ( 2 ) , EGF ( 1 ) , insulin ( 14 ) , neurotrophin-3 ( 15 ) , PDGF ( 13 , 16 ) , PS ( 5 ) , SMI11293 ( 2 ) , vanadate ( 13 )

Downstream Regulation
Effects of modification on PLCG1:
enzymatic activity, induced ( 1 , 5 , 10 , 16 ) , intracellular localization ( 5 )

References 

1

Chung SH, et al. (2010) A double point mutation in PCL-gamma1 (Y509A/F510A) enhances Y783 phosphorylation and inositol phospholipid-hydrolyzing activity upon EGF stimulation. Exp Mol Med 42, 216-27
20164676   Curated Info

2

Huang YZ, McNamara JO (2010) Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. J Biol Chem 285, 8207-17
20064930   Curated Info

3

Hu Y (2009) CST Curation Set: 8308; Year: 2009; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Tucker M (2009) CST Curation Set: 7140; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Harita Y, et al. (2009) Phosphorylation of Nephrin Triggers Ca2+ Signaling by Recruitment and Activation of Phospholipase C-{gamma}1. J Biol Chem 284, 8951-62
19179337   Curated Info

6

Guo A (2009) CST Curation Set: 5864; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Guo A (2008) CST Curation Set: 5219; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Mouneimne G, et al. (2006) Spatial and temporal control of cofilin activity is required for directional sensing during chemotaxis. Curr Biol 16, 2193-205
17113383   Curated Info

11

Guo A (2005) CST Curation Set: 678; Year: 2005; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Guo A (2005) CST Curation Set: 679; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 15'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Sekiya F, Poulin B, Kim YJ, Rhee SG (2004) Mechanism of tyrosine phosphorylation and activation of phospholipase C-gamma 1. Tyrosine 783 phosphorylation is not sufficient for lipase activation. J Biol Chem 279, 32181-90
15161916   Curated Info

14

Kwon YK, et al. (2003) Role of the pleckstrin homology domain of PLCgamma1 in its interaction with the insulin receptor. J Cell Biol 163, 375-84
14568990   Curated Info

15

Middlemas DS, Meisenhelder J, Hunter T (1994) Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor. J Biol Chem 269, 5458-66
8106527   Curated Info

16

Kim HK, et al. (1991) PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell 65, 435-41
1708307   Curated Info