Tyr771
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Home > Phosphorylation Site Page: > Tyr771  -  PLCG1 (rat)

Site Information
IGTAEPDyGALyEGR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448575

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 4 ) , mutation of modification site ( 5 , 7 ) , phospho-antibody ( 2 , 3 , 5 ) , phosphopeptide mapping ( 6 , 7 ) , western blotting ( 2 , 3 , 7 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 3 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 5 ) , 3T3 (fibroblast) ( 7 ) , brain ( 4 ) , COS (fibroblast) ( 2 ) , Null-TV1 ( 5 ) , Rat2 (fibroblast) ( 6 )

Upstream Regulation
Treatments:
BDNF ( 3 ) , EGF ( 2 ) , PDGF ( 5 , 7 ) , SMI11293 ( 3 ) , vanadate ( 5 )

Downstream Regulation
Effects of modification on PLCG1:
enzymatic activity, induced ( 7 )

References 

1

Jun HJ, et al. (2012) The oncogenic lung cancer fusion kinase CD74-ROS activates a novel invasiveness pathway through E-Syt1 phosphorylation. Cancer Res 72, 3764-74
22659450   Curated Info

2

Chung SH, et al. (2010) A double point mutation in PCL-gamma1 (Y509A/F510A) enhances Y783 phosphorylation and inositol phospholipid-hydrolyzing activity upon EGF stimulation. Exp Mol Med 42, 216-27
20164676   Curated Info

3

Huang YZ, McNamara JO (2010) Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. J Biol Chem 285, 8207-17
20064930   Curated Info

4

Guo A (2005) CST Curation Set: 679; Year: 2005; Biosample/Treatment: tissue, brain/ischemia 15'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Sekiya F, Poulin B, Kim YJ, Rhee SG (2004) Mechanism of tyrosine phosphorylation and activation of phospholipase C-gamma 1. Tyrosine 783 phosphorylation is not sufficient for lipase activation. J Biol Chem 279, 32181-90
15161916   Curated Info

6

Middlemas DS, Meisenhelder J, Hunter T (1994) Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor. J Biol Chem 269, 5458-66
8106527   Curated Info

7

Kim HK, et al. (1991) PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell 65, 435-41
1708307   Curated Info