Tyr1168

Site Information
DIyEtDyyRKGGkGL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448388

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 25 ) , [32P] bio-synthetic labeling ( 25 ) , immunoassay ( 2 , 4 ) , mass spectrometry ( 6 , 7 , 8 , 9 , 10 , 11 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 12 , 25 ) , phosphoamino acid analysis ( 25 ) , western blotting ( 1 , 3 , 12 )
Disease tissue studied:	breast cancer ( 2 ) , lung cancer ( 11 ) , non-small cell lung cancer ( 11 )
Relevant cell line - cell type - tissue:	'muscle, skeletal' ( 12 ) , 'stem, mesenchymal' ( 13 , 14 ) , 3T3 (fibroblast) [Pyk2 (human), transfection, stable Pyk2 (v5-His tagged) transfection, in RU-486 (mifepristone)-inducible expression system] ( 7 ) , 3T3-L1 (fibroblast) ( 22 ) , 831/13 ( 23 , 24 ) , C2C12-D (muscle cell) ( 21 ) , heart ( 8 , 19 ) , HEK293T (epithelial) ( 3 ) , hTERT-RPE1 (epithelial) ( 4 ) , liver ( 17 , 18 , 20 ) , liver [Akt2 (mouse), homozygous knockout] ( 15 , 16 ) , lung ( 9 , 11 ) , mammary gland ( 3 ) , MDA-MB-231 (breast cell) ( 2 ) , MEF (fibroblast) ( 10 ) , MM1.S (lymphoblast) ( 1 ) , myoblast ( 12 ) , neuron-'brain, cerebral cortex' ( 4 ) , stem ( 3 ) , SUM149 (breast cell) ( 2 ) , SYF (fibroblast) [Src iso1 (mouse), transfection] ( 6 )

Upstream Regulation
Regulatory protein:	EPCR (human) ( 3 ) , ILK (mouse) ( 12 ) , PROC (human) ( 3 )
Putative in vivo kinases:	IGF1R (mouse) ( 25 ) , Src iso1 (mouse) ( 25 )
Kinases, in vitro:	IGF1R (mouse) ( 25 ) , Src iso1 (mouse) ( 25 )
Treatments:	exercise ( 12 ) , IGF-1 ( 4 , 25 ) , insulin ( 1 ) , serum_starvation ( 4 )

Downstream Regulation
Effects of modification on IGF1R:	enzymatic activity, induced ( 25 ) , intracellular localization ( 4 )
Effects of modification on biological processes:	cell cycle regulation ( 4 ) , cell differentiation, induced ( 4 ) , cell growth, induced ( 3 , 4 )

References
1	Shah UA, et al. (2024) Prevalence and impact of diabetes on survival of patients with multiple myeloma in different racial groups. Blood Adv 8, 236-247 37772981 Curated Info
2	Sluimer LM, et al. (2023) SKOR1 mediates FER kinase-dependent invasive growth of breast cancer cells. J Cell Sci 136 36620935 Curated Info
3	Liu C, et al. (2022) Procr functions as a signaling receptor and is essential for the maintenance and self-renewal of mammary stem cells. Cell Rep 38, 110548 35320720 Curated Info
4	Yeh C, et al. (2013) IGF-1 Activates a Cilium-Localized Noncanonical Gβγ Signaling Pathway that Regulates Cell-Cycle Progression. Dev Cell 26, 358-68 23954591 Curated Info
5	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
6	Ferrando IM, et al. (2012) Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics 11, 355-69 22499769 Curated Info
7	Bonnette PC, et al. (2010) Phosphoproteomic characterization of PYK2 signaling pathways involved in osteogenesis. J Proteomics 73, 1306-20 20116462 Curated Info
8	Stokes M (2008) CST Curation Set: 5665; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
9	Rikova K (2008) CST Curation Set: 4985; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60 18563927 Curated Info
11	Rikova K (2008) CST Curation Set: 4438; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	Wang HV, et al. (2008) Integrin-linked kinase stabilizes myotendinous junctions and protects muscle from stress-induced damage. J Cell Biol 180, 1037-49 18332223 Curated Info
13	Yu J (2007) CST Curation Set: 3379; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Yu J (2007) CST Curation Set: 3380; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/bFGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Guo A (2007) CST Curation Set: 2989; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
16	Guo A (2007) CST Curation Set: 2990; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
17	Guo A (2007) CST Curation Set: 2985; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
18	Guo A (2007) CST Curation Set: 2986; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
19	Guo A (2007) CST Curation Set: 2827; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
20	Guo A (2007) CST Curation Set: 2830; Year: 2007; Biosample/Treatment: tissue, liver/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
21	Guo A (2007) CST Curation Set: 2565; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
22	Schmelzle K, et al. (2006) Temporal dynamics of tyrosine phosphorylation in insulin signaling. Diabetes 55, 2171-9 16873679 Curated Info
23	Li Y (2004) CST Curation Set: 380; Year: 2004; Biosample/Treatment: cell line, 831/13/Serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
24	Li Y (2004) CST Curation Set: 381; Year: 2004; Biosample/Treatment: cell line, 831/13/Serum starved &'\|\|' IGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
25	Peterson JE, et al. (1996) Src phosphorylates the insulin-like growth factor type I receptor on the autophosphorylation sites. Requirement for transformation by src. J Biol Chem 271, 31562-71 8940173 Curated Info