Tyr423
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Home > Phosphorylation Site Page: > Tyr423  -  SHC1 (rat)

Site Information
ELFDDPSyVNIQNLD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448511
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 9 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 10 ) , phospho-antibody ( 9 ) , western blotting ( 9 )
Disease tissue studied:
adrenal cancer ( 3 , 4 , 5 ) , pheochromocytoma ( 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
brain ( 10 ) , fibroblast-heart ( 9 ) , PC-12 (chromaffin) ( 3 , 4 , 5 , 6 , 7 , 8 ) , Rat2 (fibroblast) ( 1 )

Upstream Regulation
Treatments:
AG1478 ( 9 ) , EGF ( 9 ) , phorbol_ester ( 9 ) , PP1 ( 9 ) , thrombin ( 9 )

References 

1

Zheng Y, et al. (2013) Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Nature 499, 166-71
23846654   Curated Info

2

Jun HJ, et al. (2012) The oncogenic lung cancer fusion kinase CD74-ROS activates a novel invasiveness pathway through E-Syt1 phosphorylation. Cancer Res 72, 3764-74
22659450   Curated Info

3

Guo A (2008) CST Curation Set: 5204; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2008) CST Curation Set: 3877; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Stokes M (2007) CST Curation Set: 3524; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Stokes M (2007) CST Curation Set: 3525; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Obreztchikova M, et al. (2006) Distinct signaling functions for Shc isoforms in the heart. J Biol Chem 281, 20197-204
16699171   Curated Info

10

Guo A (2005) CST Curation Set: 678; Year: 2005; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info