Ser86
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Home > Phosphorylation Site Page: > Ser86  -  HSP27 (rat)

Site Information
RALNRQLssGVsEIR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448403

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 9 ) , mass spectrometry ( 5 , 7 , 8 , 9 , 10 , 11 ) , mass spectrometry (in vitro) ( 8 ) , mutation of modification site ( 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 9 , 12 , 13 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 9 , 12 , 13 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 5 ) , 'brain, cerebellum' ( 5 ) , 'brain, cerebral cortex' ( 1 ) , 'kidney, inner medulla' ( 10 ) , 'muscle, skeletal' ( 12 , 13 ) , 'uterus, pregnant' ( 8 ) , A10 (myoblast) ( 12 ) , adipose tissue ( 5 ) , blood ( 5 ) , cardiac-heart ( 6 ) , cerebral arteries ( 4 ) , H9c2 (myoblast) ( 9 ) , heart ( 3 , 5 ) , intestine ( 5 ) , kidney ( 5 ) , liver ( 5 , 7 ) , lung ( 5 ) , muscle ( 5 ) , pancreas ( 5 ) , retinal-retina ( 2 ) , spleen ( 5 ) , stomach ( 5 ) , testis ( 5 ) , thymus ( 5 ) , uterus ( 11 )

Upstream Regulation
Kinases, in vitro:
PKACA (cow) ( 8 )
Treatments:
cold cardioplegia followed by reperfusion ( 6 ) , adriamycin ( 9 ) , bisindolylmaleimide ( 14 ) , blood pressure ( 4 ) , cAMP_analog ( 8 ) , ciclosporin ( 4 ) , diabetes ( 3 ) , DMSO ( 1 ) , genistein ( 14 ) , Geranylgeranylacetone ( 1 ) , GF109203X ( 4 ) , H1152 ( 4 ) , H2O2 ( 14 ) , heat_shock ( 9 ) , injury ( 2 ) , ischemia/reperfusion ( 1 ) , KRIBB3 ( 4 ) , MCAO/R ( 1 ) , muscle atrophy ( 13 ) , nerve_damage ( 13 ) , PD169316 ( 12 ) , PD98059 ( 12 , 14 ) , SB203580 ( 6 , 12 , 14 ) , staurosporine ( 14 ) , V1a_vasopressin_receptor_antagonist ( 12 ) , V2_vasopressin_receptor_antagonist ( 12 ) , vasopressin ( 12 )

Downstream Regulation
Effects of modification on HSP27:
enzymatic activity, induced ( 1 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 4 )

References 

1

Matsuo K, et al. (2021) Geranylgeranylacetone attenuates cerebral ischemia-reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study. BMC Neurosci 22, 9
33557752   Curated Info

2

Schmidt T, et al. (2016) Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. Cell Stress Chaperones 21, 167-78
26475352   Curated Info

3

Reddy VS, Kumar ChU, Raghu G, Reddy GB (2014) Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions. Arch Biochem Biophys 558, 1-9
24950024   Curated Info

4

Moreno-Domínguez A, et al. (2014) Cytoskeletal Reorganization Evoked by Rho-associated kinase- and Protein Kinase C-catalyzed Phosphorylation of Cofilin and Heat Shock Protein 27, Respectively, Contributes to Myogenic Constriction of Rat Cerebral Arteries. J Biol Chem 289, 20939-20952
24914207   Curated Info

5

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

6

Clements RT, et al. (2011) p38 MAPK-dependent small HSP27 and αB-crystallin phosphorylation in regulation of myocardial function following cardioplegic arrest. Am J Physiol Heart Circ Physiol 300, H1669-77
21357508   Curated Info

7

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

8

Huang SY, et al. (2007) A systematic MS-based approach for identifying in vitro substrates of PKA and PKG in rat uteri. J Proteome Res 6, 2674-84
17564427   Curated Info

9

Venkatakrishnan CD, et al. (2006) Heat shock protects cardiac cells from doxorubicin-induced toxicity by activating p38 MAPK and phosphorylation of small heat shock protein 27. Am J Physiol Heart Circ Physiol 291, H2680-91
16782845   Curated Info

10

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info

11

Huang SY, et al. (2006) Quantitation of protein phosphorylation in pregnant rat uteri using stable isotope dimethyl labeling coupled with IMAC. Proteomics 6, 1722-34
16470654   Curated Info

12

Akamatsu S, et al. (2004) Vasopressin phosphorylates HSP27 in aortic smooth muscle cells. J Cell Biochem 92, 1203-11
15258903   Curated Info

13

Kato K, et al. (2002) Innervation-dependent phosphorylation and accumulation of alphaB-crystallin and Hsp27 as insoluble complexes in disused muscle. FASEB J 16, 1432-4
12205038   Curated Info

14

Eaton P, Fuller W, Shattock MJ (2002) S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation. J Biol Chem 277, 21189-96
11925435   Curated Info