Ser86

Site Information
RALNRQLssGVsEIR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448403

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 11 ) , immunoassay ( 2 ) , mass spectrometry ( 7 , 9 , 10 , 11 , 12 , 13 ) , mass spectrometry (in vitro) ( 10 ) , mutation of modification site ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 11 , 14 , 15 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 11 , 14 , 15 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 7 ) , 'brain, cerebellum' ( 7 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 12 ) , 'muscle, skeletal' ( 14 , 15 ) , 'uterus, pregnant' ( 10 ) , A10 (myoblast) ( 14 ) , adipose tissue ( 7 ) , blood ( 7 ) , cardiac-heart ( 8 ) , cerebral arteries ( 6 ) , H9c2 (myoblast) ( 1 , 11 ) , heart ( 5 , 7 ) , hTERT-HM (myometrial) ( 2 ) , intestine ( 7 ) , kidney ( 7 ) , liver ( 7 , 9 ) , lung ( 7 ) , muscle ( 7 ) , myometrial ( 2 ) , pancreas ( 7 ) , retinal-retina ( 4 ) , spleen ( 7 ) , stomach ( 7 ) , testis ( 7 ) , thymus ( 7 ) , uterus ( 13 )

In vivo Characterization

Methods used to characterize site in vivo:

2D analysis ( 11 ) , immunoassay ( 2 ) , mass spectrometry ( 7 , 9 , 10 , 11 , 12 , 13 ) , mass spectrometry (in vitro) ( 10 ) , mutation of modification site ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 11 , 14 , 15 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 11 , 14 , 15 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 7 ) , 'brain, cerebellum' ( 7 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 12 ) , 'muscle, skeletal' ( 14 , 15 ) , 'uterus, pregnant' ( 10 ) , A10 (myoblast) ( 14 ) , adipose tissue ( 7 ) , blood ( 7 ) , cardiac-heart ( 8 ) , cerebral arteries ( 6 ) , H9c2 (myoblast) ( 1 , 11 ) , heart ( 5 , 7 ) , hTERT-HM (myometrial) ( 2 ) , intestine ( 7 ) , kidney ( 7 ) , liver ( 7 , 9 ) , lung ( 7 ) , muscle ( 7 ) , myometrial ( 2 ) , pancreas ( 7 ) , retinal-retina ( 4 ) , spleen ( 7 ) , stomach ( 7 ) , testis ( 7 ) , thymus ( 7 ) , uterus ( 13 )

Upstream Regulation
Kinases, in vitro:	PKACA (cow) ( 10 )
Treatments:	cold cardioplegia followed by reperfusion ( 8 ) , adriamycin ( 11 ) , bisindolylmaleimide ( 16 ) , blood pressure ( 6 ) , cAMP_analog ( 10 ) , ciclosporin ( 6 ) , diabetes ( 5 ) , DMSO ( 3 ) , genistein ( 16 ) , Geranylgeranylacetone ( 3 ) , GF109203X ( 6 ) , H1152 ( 6 ) , H2O2 ( 1 , 16 ) , heat_shock ( 11 ) , injury ( 4 ) , ischemia/reperfusion ( 3 ) , KRIBB3 ( 6 ) , MCAO/R ( 3 ) , muscle atrophy ( 15 ) , nerve_damage ( 15 ) , PD169316 ( 14 ) , PD98059 ( 14 , 16 ) , pregnancy ( 2 ) , SB203580 ( 8 , 14 , 16 ) , staurosporine ( 16 ) , V1a_vasopressin_receptor_antagonist ( 14 ) , V2_vasopressin_receptor_antagonist ( 14 ) , vasopressin ( 14 )

Upstream Regulation

Kinases, in vitro:

PKACA (cow) ( 10 )

Treatments:

cold cardioplegia followed by reperfusion ( 8 ) , adriamycin ( 11 ) , bisindolylmaleimide ( 16 ) , blood pressure ( 6 ) , cAMP_analog ( 10 ) , ciclosporin ( 6 ) , diabetes ( 5 ) , DMSO ( 3 ) , genistein ( 16 ) , Geranylgeranylacetone ( 3 ) , GF109203X ( 6 ) , H1152 ( 6 ) , H2O2 ( 1 , 16 ) , heat_shock ( 11 ) , injury ( 4 ) , ischemia/reperfusion ( 3 ) , KRIBB3 ( 6 ) , MCAO/R ( 3 ) , muscle atrophy ( 15 ) , nerve_damage ( 15 ) , PD169316 ( 14 ) , PD98059 ( 14 , 16 ) , pregnancy ( 2 ) , SB203580 ( 8 , 14 , 16 ) , staurosporine ( 16 ) , V1a_vasopressin_receptor_antagonist ( 14 ) , V2_vasopressin_receptor_antagonist ( 14 ) , vasopressin ( 14 )

Downstream Regulation
Effects of modification on HSP27:	enzymatic activity, induced ( 3 ) , intracellular localization ( 2 )
Effects of modification on biological processes:	cytoskeletal reorganization ( 6 )

References
1	Wang N, et al. (2023) Homo-oxidized HSPB1 protects H9c2 cells against oxidative stress via activation of KEAP1/NRF2 signaling pathway. iScience 26, 107443 37575200 Curated Info
2	Miskiewicz EI, Olaloku A, MacPhee BK, MacPhee DJ (2022) Phosphoserine-86-HSPB1 (pS86-HSPB1) is cytoplasmic and highly induced in rat myometrium at labour. Histochem Cell Biol 36260112 Curated Info
3	Matsuo K, et al. (2021) Geranylgeranylacetone attenuates cerebral ischemia-reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study. BMC Neurosci 22, 9 33557752 Curated Info
4	Schmidt T, et al. (2016) Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. Cell Stress Chaperones 21, 167-78 26475352 Curated Info
5	Reddy VS, Kumar ChU, Raghu G, Reddy GB (2014) Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions. Arch Biochem Biophys 558, 1-9 24950024 Curated Info
6	Moreno-Domínguez A, et al. (2014) Cytoskeletal Reorganization Evoked by Rho-associated kinase- and Protein Kinase C-catalyzed Phosphorylation of Cofilin and Heat Shock Protein 27, Respectively, Contributes to Myogenic Constriction of Rat Cerebral Arteries. J Biol Chem 289, 20939-20952 24914207 Curated Info
7	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
8	Clements RT, et al. (2011) p38 MAPK-dependent small HSP27 and αB-crystallin phosphorylation in regulation of myocardial function following cardioplegic arrest. Am J Physiol Heart Circ Physiol 300, H1669-77 21357508 Curated Info
9	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
10	Huang SY, et al. (2007) A systematic MS-based approach for identifying in vitro substrates of PKA and PKG in rat uteri. J Proteome Res 6, 2674-84 17564427 Curated Info
11	Venkatakrishnan CD, et al. (2006) Heat shock protects cardiac cells from doxorubicin-induced toxicity by activating p38 MAPK and phosphorylation of small heat shock protein 27. Am J Physiol Heart Circ Physiol 291, H2680-91 16782845 Curated Info
12	Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64 16641100 Curated Info
13	Huang SY, et al. (2006) Quantitation of protein phosphorylation in pregnant rat uteri using stable isotope dimethyl labeling coupled with IMAC. Proteomics 6, 1722-34 16470654 Curated Info
14	Akamatsu S, et al. (2004) Vasopressin phosphorylates HSP27 in aortic smooth muscle cells. J Cell Biochem 92, 1203-11 15258903 Curated Info
15	Kato K, et al. (2002) Innervation-dependent phosphorylation and accumulation of alphaB-crystallin and Hsp27 as insoluble complexes in disused muscle. FASEB J 16, 1432-4 12205038 Curated Info
16	Eaton P, Fuller W, Shattock MJ (2002) S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation. J Biol Chem 277, 21189-96 11925435 Curated Info