Ser15

Site Information
FsLLRsPsWEPFRDW SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448401

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 9 ) , immunoassay ( 2 ) , mass spectrometry ( 4 , 6 , 7 , 8 , 9 , 10 , 11 ) , mass spectrometry (in vitro) ( 8 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 9 , 12 , 14 , 15 ) , western blotting ( 1 , 3 , 5 , 9 , 12 , 14 , 15 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'kidney, inner medulla' ( 7 , 10 ) , 'muscle, skeletal' ( 12 , 14 ) , 'uterus, pregnant' ( 8 ) , A10 (myoblast) ( 12 ) , adipose tissue ( 4 ) , blood ( 4 ) , cardiac-heart ( 5 ) , H9c2 (myoblast) ( 1 , 9 ) , heart ( 4 , 15 ) , hTERT-HM (myometrial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 , 6 ) , lung ( 4 ) , muscle ( 4 ) , pancreas ( 4 ) , retinal-retina ( 3 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 ) , thymus ( 4 ) , uterus ( 11 )

In vivo Characterization

Methods used to characterize site in vivo:

2D analysis ( 9 ) , immunoassay ( 2 ) , mass spectrometry ( 4 , 6 , 7 , 8 , 9 , 10 , 11 ) , mass spectrometry (in vitro) ( 8 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 9 , 12 , 14 , 15 ) , western blotting ( 1 , 3 , 5 , 9 , 12 , 14 , 15 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'kidney, inner medulla' ( 7 , 10 ) , 'muscle, skeletal' ( 12 , 14 ) , 'uterus, pregnant' ( 8 ) , A10 (myoblast) ( 12 ) , adipose tissue ( 4 ) , blood ( 4 ) , cardiac-heart ( 5 ) , H9c2 (myoblast) ( 1 , 9 ) , heart ( 4 , 15 ) , hTERT-HM (myometrial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 , 6 ) , lung ( 4 ) , muscle ( 4 ) , pancreas ( 4 ) , retinal-retina ( 3 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 ) , thymus ( 4 ) , uterus ( 11 )

Upstream Regulation
Regulatory protein:	HSP27 (human) ( 1 )
Kinases, in vitro:	PKACA (cow) ( 8 )
Treatments:	cold cardioplegia followed by reperfusion ( 5 ) , adriamycin ( 9 ) , bisindolylmaleimide ( 15 ) , cAMP_analog ( 8 ) , cGMP_analog ( 11 ) , genistein ( 15 ) , H2O2 ( 1 , 15 ) , heat_shock ( 9 ) , injury ( 3 ) , muscle atrophy ( 14 ) , nerve_damage ( 14 ) , PD169316 ( 12 ) , PD184352 ( 13 ) , PD98059 ( 12 , 15 ) , SB203580 ( 5 , 12 , 13 , 15 ) , staurosporine ( 15 ) , UV ( 13 ) , V1a_vasopressin_receptor_antagonist ( 12 ) , V2_vasopressin_receptor_antagonist ( 12 ) , vasopressin ( 12 )

Downstream Regulation
Effects of modification on HSP27:	intracellular localization ( 2 ) , protein conformation ( 1 )

References
1	Wang N, et al. (2023) Homo-oxidized HSPB1 protects H9c2 cells against oxidative stress via activation of KEAP1/NRF2 signaling pathway. iScience 26, 107443 37575200 Curated Info
2	Miskiewicz EI, Olaloku A, MacPhee BK, MacPhee DJ (2022) Phosphoserine-86-HSPB1 (pS86-HSPB1) is cytoplasmic and highly induced in rat myometrium at labour. Histochem Cell Biol 36260112 Curated Info
3	Schmidt T, et al. (2016) Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. Cell Stress Chaperones 21, 167-78 26475352 Curated Info
4	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
5	Clements RT, et al. (2011) p38 MAPK-dependent small HSP27 and αB-crystallin phosphorylation in regulation of myocardial function following cardioplegic arrest. Am J Physiol Heart Circ Physiol 300, H1669-77 21357508 Curated Info
6	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
7	Hoffert JD, et al. (2007) An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins. J Proteome Res 6, 3501-8 17683130 Curated Info
8	Huang SY, et al. (2007) A systematic MS-based approach for identifying in vitro substrates of PKA and PKG in rat uteri. J Proteome Res 6, 2674-84 17564427 Curated Info
9	Venkatakrishnan CD, et al. (2006) Heat shock protects cardiac cells from doxorubicin-induced toxicity by activating p38 MAPK and phosphorylation of small heat shock protein 27. Am J Physiol Heart Circ Physiol 291, H2680-91 16782845 Curated Info
10	Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64 16641100 Curated Info
11	Huang SY, et al. (2006) Quantitation of protein phosphorylation in pregnant rat uteri using stable isotope dimethyl labeling coupled with IMAC. Proteomics 6, 1722-34 16470654 Curated Info
12	Akamatsu S, et al. (2004) Vasopressin phosphorylates HSP27 in aortic smooth muscle cells. J Cell Biochem 92, 1203-11 15258903 Curated Info
13	Sabio G, et al. (2004) Stress- and mitogen-induced phosphorylation of the synapse-associated protein SAP90/PSD-95 by activation of SAPK3/p38gamma and ERK1/ERK2. Biochem J 380, 19-30 14741046 Curated Info
14	Kato K, et al. (2002) Innervation-dependent phosphorylation and accumulation of alphaB-crystallin and Hsp27 as insoluble complexes in disused muscle. FASEB J 16, 1432-4 12205038 Curated Info
15	Eaton P, Fuller W, Shattock MJ (2002) S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation. J Biol Chem 277, 21189-96 11925435 Curated Info