Ser649
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Home > Phosphorylation Site Page: > Ser649  -  GYS1 (mouse)

Site Information
VPPsPsLsRHssPHQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448239

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 ) , phospho-antibody ( 7 , 8 , 9 ) , western blotting ( 7 , 8 , 9 )
Disease tissue studied:
anthrax infection ( 5 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 , 3 ) , 'fat, brown' ( 6 ) , 'muscle, skeletal' ( 7 , 9 ) , heart ( 4 , 6 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 2 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 2 ) , HL-1 (myocyte) ( 2 ) , kidney ( 6 ) , muscle ( 8 ) , spleen ( 5 )

Upstream Regulation
Regulatory protein:
ADRB1 (mouse) ( 4 ) , Akt2 (mouse) ( 7 )
Treatments:
dexamethasone ( 9 ) , exercise ( 9 ) , fasting ( 8 ) , insulin ( 3 , 8 , 9 ) , LY294002 ( 3 ) , MK-2206 ( 3 ) , muscle contraction ( 7 )

References 

1

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

2

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

5

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

6

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

7

Sakamoto K, et al. (2006) Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle. Am J Physiol Endocrinol Metab 291, E1031-7
16803855   Curated Info

8

Jensen J, et al. (2006) Muscle glycogen inharmoniously regulates glycogen synthase activity, glucose uptake, and proximal insulin signaling. Am J Physiol Endocrinol Metab 290, E154-E162
16118249   Curated Info

9

Ruzzin J, Jensen J (2005) Contraction activates glucose uptake and glycogen synthase normally in muscles from dexamethasone-treated rats. Am J Physiol Endocrinol Metab 289, E241-50
15741240   Curated Info