Tyr317
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Home > Phosphorylation Site Page: > Tyr317  -  SHC1 iso2 (human)

Site Information
ELFDDPSyVNVQNLD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448511
Associated spectra:  6 CST

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 17 ) , [32P] bio-synthetic labeling ( 17 ) , immunoprecipitation ( 14 ) , mass spectrometry ( 2 , 8 , 9 ) , mutation of modification site ( 4 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , phospho-antibody ( 5 , 6 , 7 , 14 , 15 , 16 ) , phosphopeptide mapping ( 17 ) , western blotting ( 5 , 6 , 7 , 14 , 16 )
Disease tissue studied:
adrenal cancer ( 10 ) , pheochromocytoma ( 10 ) , breast cancer ( 5 , 6 ) , leukemia ( 15 ) , T cell leukemia ( 15 ) , ovarian cancer ( 6 ) , prostate cancer ( 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
EGFR (human) ( 6 ) , FRS2 (human) ( 5 ) , HER4 (human) ( 6 )
Putative in vivo kinases:
EGFR (human) ( 17 ) , Lck (human) ( 4 , 15 ) , TrkA (human) ( 10 )
Kinases, in vitro:
EGFR (human) ( 17 ) , FAK iso2 (mouse) ( 16 ) , Syk (human) ( 15 ) , ZAP70 (human) ( 15 )
Treatments:
AG1478 ( 12 ) , AG490 ( 4 ) , CXCL12 ( 4 ) , EGF ( 17 ) , FGF1 ( 5 ) , fibronectin ( 14 ) , heregulin ( 5 ) , insulin ( 13 ) , okadaic_acid ( 6 ) , PP1 ( 12 ) , PP2 ( 4 ) , PTX ( 4 ) , siRNA ( 4 ) , thrombin ( 7 , 12 )

Downstream Regulation
Effects of modification on SHC1 iso2:
activity, induced ( 12 , 17 ) , molecular association, regulation ( 4 , 10 , 11 , 13 , 14 ) , phosphorylation ( 11 , 14 , 16 )
Effects of modification on biological processes:
apoptosis, inhibited ( 10 ) , cell differentiation, altered ( 10 ) , cell growth, altered ( 10 ) , cell growth, induced ( 17 ) , cell motility, altered ( 4 ) , cytoskeletal reorganization ( 4 ) , signaling pathway regulation ( 14 , 17 )
Induce interaction with:
GRB2 (human) ( 4 , 10 , 13 , 14 ) , GRB2 (mouse) ( 11 ) , PIK3R1 (mouse) ( 11 ) , SHIP (mouse) ( 11 ) , SOS1 (mouse) ( 11 ) , VAV1 (human) ( 4 ) , ZAP70 (human) ( 4 )

References 

1

Deb TB, et al. (2014) PTEN-mediated ERK1/2 inhibition and paradoxical cellular proliferation following Pnck overexpression. Cell Cycle 13, 961-73
24552815   Curated Info

2

Possemato A (2009) CST Curation Set: 5935; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Stokes M (2008) CST Curation Set: 4553; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Patrussi L, et al. (2007) p52Shc is required for CXCR4-dependent signaling and chemotaxis in T cells. Blood 110, 1730-8
17537990   Curated Info

5

Manuvakhova M, et al. (2006) Expression of the SNT-1/FRS2 phosphotyrosine binding domain inhibits activation of MAP kinase and PI3-kinase pathways and antiestrogen resistant growth induced by FGF-1 in human breast carcinoma cells. Oncogene 25, 6003-14
16682955   Curated Info

6

Yumoto N, Yu X, Hatakeyama M (2006) Expression of the ErbB4 receptor causes reversal regulation of PP2A in the Shc signal transduction pathway in human cancer cells. Mol Cell Biochem 285, 165-71
16477370   Curated Info

7

Vijayan KV, et al. (2005) The Pro33 isoform of integrin beta3 enhances outside-in signaling in human platelets by regulating the activation of serine/threonine phosphatases. J Biol Chem 280, 21756-62
15826939   Curated Info

8

Gu T (2005) CST Curation Set: 5404; Year: 2005; Biosample/Treatment: cell line, 293T/bFGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Gu T (2005) CST Curation Set: 5390; Year: 2005; Biosample/Treatment: cell line, 293T/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Roccato E, et al. (2002) Biological activity of the thyroid TRK-T3 oncogene requires signalling through Shc. Br J Cancer 87, 645-53
12237775   Curated Info

11

Velazquez L, et al. (2000) The shc adaptor protein forms interdependent phosphotyrosine-mediated protein complexes in mast cells stimulated with interleukin 3. Blood 96, 132-8
10891441   Curated Info

12

Ricketts WA, Brown JH, Olefsky JM (1999) Pertussis toxin-sensitive and -insensitive thrombin stimulation of Shc phosphorylation and mitogenesis are mediated through distinct pathways. Mol Endocrinol 13, 1988-2001
10598576   Curated Info

13

Ishihara H, et al. (1998) Relative involvement of Shc tyrosine 239/240 and tyrosine 317 on insulin induced mitogenic signaling in rat1 fibroblasts expressing insulin receptors. Biochem Biophys Res Commun 252, 139-44
9813159   Curated Info

14

Wary KK, Mariotti A, Zurzolo C, Giancotti FG (1998) A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growth. Cell 94, 625-34
9741627   Curated Info

15

Walk SF, March ME, Ravichandran KS (1998) Roles of Lck, Syk and ZAP-70 tyrosine kinases in TCR-mediated phosphorylation of the adapter protein Shc. Eur J Immunol 28, 2265-75
9710204   Curated Info

16

Schlaepfer DD, Jones KC, Hunter T (1998) Multiple Grb2-mediated integrin-stimulated signaling pathways to ERK2/mitogen-activated protein kinase: summation of both c-Src- and focal adhesion kinase-initiated tyrosine phosphorylation events. Mol Cell Biol 18, 2571-85
9566877   Curated Info

17

Gotoh N, Toyoda M, Shibuya M (1997) Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation. Mol Cell Biol 17, 1824-31
9121430   Curated Info