Thr183
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Home > Phosphorylation Site Page: > Thr183  -  JNK2 (rat)

Site Information
ACTNFMMtPyVVTRY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447649

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , phospho-antibody ( 12 , 13 , 14 , 15 ) , western blotting ( 13 )
Disease tissue studied:
Alzheimer's disease ( 12 ) , adrenal cancer ( 9 , 10 ) , pheochromocytoma ( 9 , 10 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 11 ) , 'neuron, cerebellar granule'-brain ( 12 ) , 'neuron, cortical'-brain ( 14 ) , brain ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , liver ( 15 ) , PC-12 (chromaffin) ( 9 , 10 ) , RIN (epithelial) ( 13 )

Upstream Regulation
Treatments:
anoxia ( 12 ) , azaserine ( 13 ) , beta-amyloid_40 ( 14 ) , ethanol ( 15 ) , glucosamine ( 13 ) , glucose ( 13 ) , JNK_inhibitor_I ( 13 ) , retinoic_acid ( 15 )

References 

1

Hu Y (2010) CST Curation Set: 8874; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Hu Y (2010) CST Curation Set: 8921; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Hu Y (2010) CST Curation Set: 8931; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Hu Y (2010) CST Curation Set: 8923; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Hu Y (2010) CST Curation Set: 8875; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Hu Y (2010) CST Curation Set: 8920; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Hu Y (2010) CST Curation Set: 8881; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Hu Y (2010) CST Curation Set: 8880; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info

12

Liu R, et al. (2005) Acute anoxia induces tau dephosphorylation in rat brain slices and its possible underlying mechanisms. J Neurochem 94, 1225-34
15992372   Curated Info

13

Andreozzi F, et al. (2004) Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells. Endocrinology 145, 2845-57
15001544   Curated Info

14

Fogarty MP, Downer EJ, Campbell V (2003) A role for c-Jun N-terminal kinase 1 (JNK1), but not JNK2, in the beta-amyloid-mediated stabilization of protein p53 and induction of the apoptotic cascade in cultured cortical neurons. Biochem J 371, 789-98
12534344   Curated Info

15

Chung J, Chavez PR, Russell RM, Wang XD (2002) Retinoic acid inhibits hepatic Jun N-terminal kinase-dependent signaling pathway in ethanol-fed rats. Oncogene 21, 1539-47
11896582   Curated Info