Thr183
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Home > Phosphorylation Site Page: > Thr183  -  JNK2 (mouse)

Site Information
ACTNFMMtPyVVtRY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447649

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 24 ) , immunoprecipitation ( 24 , 35 ) , mass spectrometry ( 1 , 4 , 7 , 8 , 9 , 10 , 11 , 16 , 17 , 18 , 19 , 21 , 22 , 25 , 29 , 30 , 32 ) , mutation of modification site ( 20 , 39 ) , phospho-antibody ( 2 , 6 , 12 , 15 , 20 , 23 , 24 , 27 , 28 , 31 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ) , western blotting ( 2 , 6 , 12 , 15 , 20 , 23 , 24 , 27 , 28 , 31 , 33 , 34 , 37 , 39 , 40 , 41 )
Disease tissue studied:
brain cancer ( 20 ) , glioma ( 20 ) , leukemia ( 17 , 18 , 19 ) , lung cancer ( 28 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 22 , 29 ) , 293 (epithelial) ( 24 ) , 32D (myeloid) [IRS1 (mouse)] ( 40 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 16 ) , 32Dcl3 (myeloid) ( 16 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 38 ) , 3T3 (fibroblast) ( 12 ) , aorta ( 31 ) , B lymphocyte-spleen ( 33 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , brain ( 6 , 8 , 9 , 21 , 25 , 30 ) , C6 (glial) ( 20 ) , embryo ( 9 ) , ES (stem) ( 24 ) , HEK293T (epithelial) ( 2 ) , liver ( 9 , 32 ) , lung ( 28 ) , macrophage-peritoneum ( 41 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 7 ) , mast-bone marrow ( 33 ) , MEF (fibroblast) ( 27 , 34 , 39 ) , MEF (fibroblast) [DOK1 (mouse)] ( 23 ) , MEF (fibroblast) [IGF1R (mouse)] ( 36 ) , MEF (fibroblast) [PKR (mouse), homozygous knockout] ( 15 ) , RAW 264 (macrophage) ( 37 ) , RAW 264.7 (macrophage) ( 4 , 17 , 18 , 19 ) , T lymphocyte-lymph node ( 33 ) , U2OS (bone cell) [GR (human)] ( 35 )

Upstream Regulation
Regulatory protein:
Bcl-10 (mouse) ( 33 ) , CARD11 (mouse) ( 33 ) , Cot (mouse) ( 36 ) , Fyn (mouse) ( 2 ) , MEK1 (mouse) ( 24 ) , PKR (mouse) ( 15 ) , ROCK1 (human) ( 26 ) , Src (mouse) ( 12 )
Putative in vivo kinases:
MKK4 (human) ( 24 , 35 ) , MKK7 (human) ( 35 )
Kinases, in vitro:
MKK4 (mouse) ( 24 )
Treatments:
activin ( 24 ) , adriamycin ( 15 ) , anisomycin ( 41 ) , Aplidin ( 39 ) , benzo(a)pyrene ( 28 ) , etoposide ( 20 ) , HA-1077 ( 26 ) , IL-1b ( 36 ) , IL-33 ( 4 ) , IL-6 ( 31 ) , insulin ( 40 ) , ionomycin ( 33 ) , JNK_inhibitor_I ( 31 ) , linoleic_acid ( 38 ) , LY294002 ( 40 ) , MK-591 ( 6 ) , nerve_damage ( 3 ) , NTCU ( 28 ) , okadaic_acid ( 41 ) , palmitate ( 12 ) , PD98059 ( 31 , 40 ) , PGE2 ( 37 ) , phorbol_ester ( 33 , 35 , 41 ) , pomegranate_wine ( 28 ) , RANKL ( 37 ) , rapamycin ( 2 ) , TGF-alpha ( 24 ) , TGF-beta ( 24 ) , thapsigargin ( 2 ) , TNF ( 27 , 35 , 36 ) , UV ( 34 , 35 ) , zymosan ( 41 )

Downstream Regulation
Effects of modification on JNK2:
enzymatic activity, induced ( 24 , 35 )
Effects of modification on biological processes:
apoptosis, induced ( 2 ) , cell motility, induced ( 24 ) , transcription, inhibited ( 24 )

References 

1

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

2

Wang Y, Yamada E, Zong H, Pessin JE (2015) Fyn Activation of mTORC1 Stimulates the IRE1α-JNK Pathway, Leading to Cell Death. J Biol Chem 290, 24772-83
26306048   Curated Info

3

Yang J, et al. (2015) Pathological axonal death through a MAPK cascade that triggers a local energy deficit. Cell 160, 161-76
25594179   Curated Info

4

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Chu J, Lauretti E, Di Meco A, Praticò D (2013) FLAP pharmacological blockade modulates metabolism of endogenous tau in vivo . Transl Psychiatry 3, e333
24301651   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

9

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

10

Ren H (2011) CST Curation Set: 12896; Year: 2011; Biosample/Treatment: /untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Ren H (2011) CST Curation Set: 12897; Year: 2011; Biosample/Treatment: /untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Holzer RG, et al. (2011) Saturated fatty acids induce c-Src clustering within membrane subdomains, leading to JNK activation. Cell 147, 173-84
21962514   Curated Info

13

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

14

Bard-Chapeau EA, et al. (2011) Ptpn11/Shp2 acts as a tumor suppressor in hepatocellular carcinogenesis. Cancer Cell 19, 629-39
21575863   Curated Info

15

Peidis P, et al. (2011) Doxorubicin bypasses the cytoprotective effects of eIF2α phosphorylation and promotes PKR-mediated cell death. Cell Death Differ 18, 145-54
20559319   Curated Info

16

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

17

Possemato A (2009) CST Curation Set: 7168; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/LPS; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

18

Possemato A (2009) CST Curation Set: 7169; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/MG132; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

19

Possemato A (2009) CST Curation Set: 7167; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/LPS; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

20

Lomonaco SL, et al. (2008) Phosphorylation of protein kinase Cdelta on distinct tyrosine residues induces sustained activation of Erk1/2 via down-regulation of MKP-1: role in the apoptotic effect of etoposide. J Biol Chem 283, 17731-9
18434324   Curated Info

21

Zhou J (2008) CST Curation Set: 4322; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY
Curated Info

22

Zhou J (2008) CST Curation Set: 4323; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY
Curated Info

23

Hosooka T, et al. (2008) Dok1 mediates high-fat diet-induced adipocyte hypertrophy and obesity through modulation of PPAR-gamma phosphorylation. Nat Med 14, 188-93
18204460   Curated Info

24

Takatori A, et al. (2008) Differential transmission of MEKK1 morphogenetic signals by JNK1 and JNK2. Development 135, 23-32
18032450   Curated Info

25

Ballif BA, Carey GR, Sunyaev SR, Gygi SP (2008) Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain. J Proteome Res 7, 311-8
18034455   Curated Info

26

Ongusaha PP, et al. (2008) Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage. Sci Signal 1, ra14
19036714   Curated Info

27

Schlaepfer DD, et al. (2007) Tumor necrosis factor-alpha stimulates focal adhesion kinase activity required for mitogen-activated kinase-associated interleukin 6 expression. J Biol Chem 282, 17450-9
17438336   Curated Info

28

Khan N, et al. (2007) Oral consumption of pomegranate fruit extract inhibits growth and progression of primary lung tumors in mice. Cancer Res 67, 3475-82
17389758   Curated Info

29

Guo A (2007) CST Curation Set: 2672; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

30

Guo A (2007) CST Curation Set: 2675; Year: 2007; Biosample/Treatment: tissue, brain/LY; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

31

Andreozzi F, et al. (2007) Interleukin-6 impairs the insulin signaling pathway, promoting production of nitric oxide in human umbilical vein endothelial cells. Mol Cell Biol 27, 2372-83
17242212   Curated Info

32

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

33

Blonska M, et al. (2007) The CARMA1-Bcl10 signaling complex selectively regulates JNK2 kinase in the T cell receptor-signaling pathway. Immunity 26, 55-66
17189706   Curated Info

34

Jaeschke A, et al. (2006) JNK2 is a positive regulator of the cJun transcription factor. Mol Cell 23, 899-911
16973441   Curated Info

35

López-Bergami P, et al. (2005) RACK1 mediates activation of JNK by protein kinase C [corrected] Mol Cell 19, 309-20
16061178   Curated Info

36

Das S, et al. (2005) Tpl2/cot signals activate ERK, JNK, and NF-kappaB in a cell-type and stimulus-specific manner. J Biol Chem 280, 23748-57
15833743   Curated Info

37

Kobayashi Y, et al. (2005) Prostaglandin E2 enhances osteoclastic differentiation of precursor cells through protein kinase A-dependent phosphorylation of TAK1. J Biol Chem 280, 11395-403
15647289   Curated Info

38

Gao Z, et al. (2004) Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol Endocrinol 18, 2024-34
15143153   Curated Info

39

Cuadrado A, et al. (2004) JNK activation is critical for Aplidin-induced apoptosis. Oncogene 23, 4673-80
15122339   Curated Info

40

Lee YH, Giraud J, Davis RJ, White MF (2003) c-Jun N-terminal kinase (JNK) mediates feedback inhibition of the insulin signaling cascade. J Biol Chem 278, 2896-902
12417588   Curated Info

41

Gijón MA, et al. (2000) Cytosolic phospholipase A2 is required for macrophage arachidonic acid release by agonists that Do and Do not mobilize calcium. Novel role of mitogen-activated protein kinase pathways in cytosolic phospholipase A2 regulation. J Biol Chem 275, 20146-56
10867029   Curated Info