Ser326
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Home > Phosphorylation Site Page: > Ser326  -  FOXO1A (mouse)

Site Information
stISGRLsPIMtEQD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448076

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 7 , 8 , 10 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 ) , phosphopeptide mapping ( 5 ) , western blotting ( 1 , 5 )
Disease tissue studied:
anthrax infection ( 8 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 ) , fibroblast-lung ( 10 ) , hepatocyte-liver ( 5 ) , kidney ( 1 ) , liver ( 2 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) ( 7 ) , spleen ( 8 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (mouse) ( 1 ) , ERK2 (mouse) ( 1 , 11 ) , P38A (mouse) ( 11 )
Treatments:
insulin ( 3 ) , LY294002 ( 3 ) , Trametinib ( 1 )

Downstream Regulation
Effects of modification on FOXO1A:
intracellular localization ( 5 )
Effects of modification on biological processes:
transcription, induced ( 1 )

References 

1

Collier JB, Whitaker RM, Eblen ST, Schnellmann RG (2016) Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor γ Coactivator-1α by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions. J Biol Chem 291, 26850-26859
27875304   Curated Info

2

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

5

Ozcan L, et al. (2012) Calcium signaling through CaMKII regulates hepatic glucose production in fasting and obesity. Cell Metab 15, 739-51
22503562   Curated Info

6

Jiao P, Feng B, Xu H (2012) Mapping MKP-3/FOXO1 Interaction and Evaluating the Effect on Gluconeogenesis. PLoS One 7, e41168
22848439   Curated Info

7

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

8

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

9

Kim S, Kim Y, Lee J, Chung J (2010) Regulation of FOXO1 by TAK1-Nemo-like kinase pathway. J Biol Chem 285, 8122-9
20061393   Curated Info

10

Guo A (2009) CST Curation Set: 6840; Year: 2009; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info

11

Asada S, et al. (2007) Mitogen-activated protein kinases, Erk and p38, phosphorylate and regulate Foxo1. Cell Signal 19, 519-27
17113751   Curated Info