Tyr402
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Home > Phosphorylation Site Page: > Tyr402  -  Pyk2 (rat)

Site Information
CSIESDIyAEIPDET   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447955

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 4 , 15 ) , mutation of modification site ( 1 , 9 , 11 , 15 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 10 , 11 , 12 , 13 , 15 )
Disease tissue studied:
bone cancer ( 11 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 4 , 7 , 8 , 10 , 16 ) , 293 (epithelial) ( 15 ) , granulosa ( 14 ) , L3.6p1 (pancreatic) ( 1 ) , myocyte-heart ( 9 ) , neuron-'brain, hippocampus' ( 3 ) , pancreas ( 13 ) , pancreatic acini ( 5 ) , ROS 17/2.8 (bone cell) ( 11 ) , small mesenteric arteries ( 6 )

Upstream Regulation
Regulatory protein:
Fyn (mouse) ( 8 ) , PSD-95 (human) ( 4 )
Kinases, in vitro:
Pyk2 (rat) ( 4 )
Putative upstream phosphatases:
PTPRA (rat) ( 15 )
Phosphatases, in vitro:
PTPRA (rat) ( 15 )
Treatments:
BAPTA-AM ( 11 ) , Ca(2+) ( 2 ) , calmidazolium ( 4 ) , CCK-JMV ( 13 ) , cholecystokinin ( 5 , 13 ) , cytochalasin_D ( 6 ) , depolarization ( 2 , 8 ) , EGTA ( 11 ) , electrical_stimulation ( 16 ) , endothelin ( 6 ) , GF109203X ( 5 , 12 , 13 ) , ionomycin ( 2 ) , ischemia ( 10 , 12 ) , ischemia/reperfusion ( 10 ) , lithium ( 10 ) , mechanical stress ( 11 ) , ML-9 ( 2 ) , nifedipine ( 2 ) , NMDA ( 3 , 4 ) , norepinephrine ( 6 ) , NVP-TAE226 ( 2 ) , PF-431396 ( 2 ) , PF-573228 ( 2 ) , phorbol_ester ( 5 ) , PP2 ( 8 ) , PP3 ( 8 ) , sc-203950 ( 2 ) , status epilepticus ( 7 ) , thapsigargin ( 5 , 11 , 13 ) , trifluoperazine ( 4 ) , W-7 ( 4 )

Downstream Regulation
Effects of modification on Pyk2:
molecular association, regulation ( 11 ) , phosphorylation ( 9 ) , protein conformation ( 4 )
Effects of modification on biological processes:
apoptosis, altered ( 9 ) , cell adhesion, altered ( 9 ) , cytoskeletal reorganization ( 9 ) , neural plasticity ( 3 )
Induce interaction with:
FAK (rat) ( 11 )

References 

1

Huang H, et al. (2016) Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1. J Biol Chem 291, 23208-23223
27605668   Curated Info

2

Mills RD, et al. (2015) A Role for the Tyrosine Kinase Pyk2 in Depolarization-induced Contraction of Vascular Smooth Muscle. J Biol Chem 290, 8677-92
25713079   Curated Info

3

Hsin H, Kim MJ, Wang CF, Sheng M (2010) Proline-rich tyrosine kinase 2 regulates hippocampal long-term depression. J Neurosci 30, 11983-93
20826662   Curated Info

4

Bartos JA, et al. (2010) Postsynaptic clustering and activation of Pyk2 by PSD-95. J Neurosci 30, 449-63
20071509   Curated Info

5

Berna MJ, et al. (2007) CCK causes PKD1 activation in pancreatic acini by signaling through PKC-delta and PKC-independent pathways. Biochim Biophys Acta 1773, 483-501
17306383   Curated Info

6

Ohanian V, Gatfield K, Ohanian J (2005) Role of the actin cytoskeleton in G-protein-coupled receptor activation of PYK2 and paxillin in vascular smooth muscle. Hypertension 46, 93-9
15911746   Curated Info

7

Niimura M, et al. (2005) Changes in phosphorylation of the NMDA receptor in the rat hippocampus induced by status epilepticus. J Neurochem 92, 1377-85
15748156   Curated Info

8

Corvol JC, et al. (2005) Depolarization activates ERK and proline-rich tyrosine kinase 2 (PYK2) independently in different cellular compartments in hippocampal slices. J Biol Chem 280, 660-8
15537634   Curated Info

9

Melendez J, et al. (2004) Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin. J Biol Chem 279, 53516-23
15322113   Curated Info

10

Ma J, et al. (2004) Lithium suppressed Tyr-402 phosphorylation of proline-rich tyrosine kinase (Pyk2) and interactions of Pyk2 and PSD-95 with NR2A in rat hippocampus following cerebral ischemia. Neurosci Res 49, 357-62
15236860   Curated Info

11

Boutahar N, Guignandon A, Vico L, Lafage-Proust MH (2004) Mechanical strain on osteoblasts activates autophosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 tyrosine sites involved in ERK activation. J Biol Chem 279, 30588-99
15096502   Curated Info

12

Cheung HH, Teves L, Wallace MC, Gurd JW (2003) Inhibition of protein kinase C reduces ischemia-induced tyrosine phosphorylation of the N-methyl-d-aspartate receptor. J Neurochem 86, 1441-9
12950452   Curated Info

13

Pace A, et al. (2003) Phosphospecific site tyrosine phosphorylation of p125FAK and proline-rich kinase 2 is differentially regulated by cholecystokinin receptor type A activation in pancreatic acini. J Biol Chem 278, 19008-16
12651850   Curated Info

14

Shiota M, et al. (2003) Protein tyrosine phosphatase PTP20 induces actin cytoskeleton reorganization by dephosphorylating p190 RhoGAP in rat ovarian granulosa cells stimulated with follicle-stimulating hormone. Mol Endocrinol 17, 534-49
12554790   Curated Info

15

Lyons PD, Dunty JM, Schaefer EM, Schaller MD (2001) Inhibition of the catalytic activity of cell adhesion kinase beta by protein-tyrosine phosphatase-PEST-mediated dephosphorylation. J Biol Chem 276, 24422-31
11337490   Curated Info

16

Jeon SH, et al. (2001) Electroconvulsive shock increases the phosphorylation of Pyk2 in the rat hippocampus. Biochem Biophys Res Commun 282, 1026-30
11352655   Curated Info

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