Ser454

Site Information
PCQNKtPsPPCPANK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4274794

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 )
Disease tissue studied:	leukemia ( 4 ) , acute myelogenous leukemia ( 4 )
Relevant cell line - cell type - tissue:	blood ( 4 ) , macrophage-peritoneum ( 3 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 2 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 2 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) ( 2 ) , spleen ( 6 ) , testis ( 6 )

Upstream Regulation
Regulatory protein:	Raptor (mouse) ( 2 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
3	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
4	Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27 22802335 Curated Info
5	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info