Ser309

Site Information
RMVtGNGsLKRKSSR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4274110

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 )
Disease tissue studied:	breast cancer ( 8 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 ) , gastric cancer ( 13 ) , gastric carcinoma ( 13 ) , leukemia ( 9 ) , acute myelogenous leukemia ( 9 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 )
Relevant cell line - cell type - tissue:	A498 (renal) ( 12 ) , breast ( 2 ) , BT-20 (breast cell) ( 8 ) , Calu 6 (pulmonary) ( 8 ) , H2077 (pulmonary) ( 8 ) , H322M (pulmonary) ( 8 ) , HeLa (cervical) ( 3 , 7 , 11 ) , HeLa S3 (cervical) ( 14 ) , HUES-9 ('stem, embryonic') ( 10 ) , Jurkat (T lymphocyte) ( 5 ) , K562 (erythroid) ( 7 ) , KG-1 (myeloid) ( 9 ) , MDA-MB-468 (breast cell) ( 8 ) , NCI-H157 (pulmonary) ( 8 ) , NCI-H1648 (pulmonary) ( 8 ) , NCI-H1666 (pulmonary) ( 8 ) , NCI-H520 (squamous) ( 8 ) , PC9 (pulmonary) ( 8 ) , stomach ( 13 )

Upstream Regulation
Treatments:	BI2536 ( 11 ) , MLN8054 ( 11 )

References
1	Amar-Schwartz A, et al. (2022) S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. Elife 11 36189922 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
6	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
7	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
8	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
9	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
10	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
11	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
12	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
13	Ren H (2008) CST Curation Set: 5625; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48 18691976 Curated Info